6mpt - Revision history

OCA at 06:36, 11 October 2023

OCA — Wed, 11 Oct 2023 06:36:27 GMT

←Older revision		Revision as of 06:36, 11 October 2023
Line 3:		Line 3:
	<StructureSection load='6mpt' size='340' side='right'caption='[[6mpt]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='6mpt' size='340' side='right'caption='[[6mpt]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mpt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"vibrio_subtilis"_ehrenberg_1835 "vibrio~~ subtilis~~" ehrenberg 1835~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MPT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MPT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mpt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MPT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C30:2-(acetylamino)-2-deoxy-1-O-[(S)-{[(R)-{[(2Z,6Z,10E,14E,18E)-3,7,11,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaen-1-yl]oxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]-alpha-D-glucopyranose'>C30</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.649Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6mps\|6mps]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C30:2-(acetylamino)-2-deoxy-1-O-[(S)-{[(R)-{[(2Z,6Z,10E,14E,18E)-3,7,11,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaen-1-yl]oxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]-alpha-D-glucopyranose'>C30</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tagT, ywtF, BSU35840 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mpt OCA], [https://pdbe.org/6mpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mpt RCSB], [https://www.ebi.ac.uk/pdbsum/6mpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mpt ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6mpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mpt OCA], [~~http~~://pdbe.org/6mpt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6mpt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6mpt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6mpt ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TAGT_BACSU TAGT_BACSU]] May catalyze the final step in cell wall teichoic acid biosynthesis, the transfer of the anionic cell wall polymers (APs) from their lipid-linked precursor to the cell wall peptidoglycan (PG).<ref>PMID:21964069</ref>	+	[https://www.uniprot.org/uniprot/TAGT_BACSU TAGT_BACSU] May catalyze the final step in cell wall teichoic acid biosynthesis, the transfer of the anionic cell wall polymers (APs) from their lipid-linked precursor to the cell wall peptidoglycan (PG).<ref>PMID:21964069</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio~~ subtilis ~~ehrenberg 1835~~]]	+	[[Category: Bacillus subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kahne, D]]	+	[[Category: Kahne D]]
-	[[Category: Owens~~, T W~~]]	+	[[Category: Owens TW]]
-	[[Category: Schaefer, K]]	+	[[Category: Schaefer K]]
-	[[Category: Walker, S]]	+	[[Category: Walker S]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Lcp]]~~	+
-	~~[[Category: Lytr-cps2a-psr]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 11:02, 18 December 2019

OCA — Wed, 18 Dec 2019 11:02:43 GMT

←Older revision		Revision as of 11:02, 18 December 2019
Line 1:		Line 1:

	==TagT bound to LI-WTA==		==TagT bound to LI-WTA==
-	<StructureSection load='6mpt' size='340' side='right' caption='[[6mpt]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='6mpt' size='340' side='right'caption='[[6mpt]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mpt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MPT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mpt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MPT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C30:2-(acetylamino)-2-deoxy-1-O-[(S)-{[(R)-{[(2Z,6Z,10E,14E,18E)-3,7,11,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaen-1-yl]oxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]-alpha-D-glucopyranose'>C30</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C30:2-(acetylamino)-2-deoxy-1-O-[(S)-{[(R)-{[(2Z,6Z,10E,14E,18E)-3,7,11,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaen-1-yl]oxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]-alpha-D-glucopyranose'>C30</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6mps\|6mps]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6mps\|6mps]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tagT, ywtF, BSU35840 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mpt OCA], [http://pdbe.org/6mpt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mpt RCSB], [http://www.ebi.ac.uk/pdbsum/6mpt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mpt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mpt OCA], [http://pdbe.org/6mpt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mpt RCSB], [http://www.ebi.ac.uk/pdbsum/6mpt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mpt ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Vibrio subtilis ehrenberg 1835]]
		+	[[Category: Large Structures]]
	[[Category: Kahne, D]]		[[Category: Kahne, D]]
	[[Category: Owens, T W]]		[[Category: Owens, T W]]

OCA: Protected "6mpt" [edit=sysop:move=sysop]

OCA — Wed, 17 Oct 2018 08:14:58 GMT

Protected "6mpt" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:14, 17 October 2018

OCA: New page: ==TagT bound to LI-WTA== == Structural highlights == <...
OCA — Wed, 17 Oct 2018 08:14:56 GMT
New page: ==TagT bound to LI-WTA== <StructureSection load='6mpt' size='340' side='right' caption='6mpt, resolution 1.65Å' scene=''> == Structural highlights == <table><tr><...
New page

==TagT bound to LI-WTA==
<StructureSection load='6mpt' size='340' side='right' caption='[[6mpt]], [[Resolution|resolution]] 1.65Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6mpt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MPT FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C30:2-(acetylamino)-2-deoxy-1-O-[(S)-{[(R)-{[(2Z,6Z,10E,14E,18E)-3,7,11,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaen-1-yl]oxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]-alpha-D-glucopyranose'>C30</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6mps|6mps]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mpt OCA], [http://pdbe.org/6mpt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mpt RCSB], [http://www.ebi.ac.uk/pdbsum/6mpt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mpt ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/TAGT_BACSU TAGT_BACSU]] May catalyze the final step in cell wall teichoic acid biosynthesis, the transfer of the anionic cell wall polymers (APs) from their lipid-linked precursor to the cell wall peptidoglycan (PG).<ref>PMID:21964069</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Gram-positive bacterial cell wall is a large supramolecular structure and its assembly requires coordination of complex biosynthetic pathways. In the step that merges the two major biosynthetic pathways in Staphylococcus aureus cell wall assembly, conserved protein ligases attach wall teichoic acids to peptidoglycan, but the order of biosynthetic events is a longstanding question. Here, we use a chemical approach to define which of the possible peptidoglycan intermediates are substrates for wall-teichoic acid ligases, thereby establishing the order of cell wall assembly. We have developed a strategy to make defined glycan chain-length polymers of either un-cross-linked or cross-linked peptidoglycan, and we find that wall teichoic acid ligases cannot transfer wall teichoic acid precursors to the cross-linked substrates. A 1.9 A crystal structure of a LytR-CpsA-Psr (LCP) family ligase in complex with a wall teichoic acid precursor defines the location of the peptidoglycan binding site as a long, narrow groove, and suggests that the basis for selectivity is steric exclusion of cross-linked peptidoglycan. Consistent with this hypothesis, we have found that chitin oligomers are good substrates for transfer, showing that LCPs do not discriminate cross-linked from un-cross-linked peptidoglycan substrates by recognizing features of the un-cross-linked stem peptide. We conclude that wall teichoic acids are coupled to un-cross-linked peptidoglycan chains at an early stage of peptidoglycan synthesis and may create marks that define the proper spacing of subsequent cross-links.

Substrate Preferences Establish the Order of Cell Wall Assembly in Staphylococcus aureus.,Schaefer K, Owens TW, Kahne D, Walker S J Am Chem Soc. 2018 Feb 21;140(7):2442-2445. doi: 10.1021/jacs.7b13551. Epub 2018, Feb 9. PMID:29402087<ref>PMID:29402087</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6mpt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Kahne, D]]
[[Category: Owens, T W]]
[[Category: Schaefer, K]]
[[Category: Walker, S]]
[[Category: Complex]]
[[Category: Lcp]]
[[Category: Lytr-cps2a-psr]]
[[Category: Transferase]]