6mre - Revision history

OCA at 06:37, 11 October 2023

OCA — Wed, 11 Oct 2023 06:37:24 GMT

←Older revision		Revision as of 06:37, 11 October 2023
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	<StructureSection load='6mre' size='340' side='right'caption='[[6mre]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='6mre' size='340' side='right'caption='[[6mre]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mre]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MRE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MRE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mre]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MRE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MRE FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=PLP:PYRIDOXAL~~-5~~-PHOSPHATE'>PLP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat">[[6mr2\|6mr2]], [[6mr6\|6mr6]], [[6mrh\|6mrh]], [[6mri\|6mri]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mre OCA], [https://pdbe.org/6mre PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mre RCSB], [https://www.ebi.ac.uk/pdbsum/6mre PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mre ProSAT]</span></td></tr>
-	~~<tr id='gene~~'>~~<td class="sblockLbl"><b>[[Gene\|Gene:]]~~</b>~~</td><td class="sblockDat">sufS, csdB, ynhB, b1680, JW1670 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6mre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mre OCA], [~~http~~://pdbe.org/6mre PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6mre RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6mre PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6mre ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SUFS_ECOLI SUFS_ECOLI]] Cysteine desulfurases mobilize the sulfur from L-cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Acts as a potent selenocysteine lyase in vitro, that mobilizes selenium from L-selenocysteine. Selenocysteine lyase activity is however unsure in vivo.<ref>PMID:10829016</ref> <ref>PMID:12089140</ref> <ref>PMID:11997471</ref> <ref>PMID:12876288</ref> <ref>PMID:12941942</ref>	+	[https://www.uniprot.org/uniprot/SUFS_ECOLI SUFS_ECOLI] Cysteine desulfurases mobilize the sulfur from L-cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Acts as a potent selenocysteine lyase in vitro, that mobilizes selenium from L-selenocysteine. Selenocysteine lyase activity is however unsure in vivo.<ref>PMID:10829016</ref> <ref>PMID:12089140</ref> <ref>PMID:11997471</ref> <ref>PMID:12876288</ref> <ref>PMID:12941942</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	==See Also==		==See Also==
	*[[Cysteine desulfurase 3D structures\|Cysteine desulfurase 3D structures]]		*[[Cysteine desulfurase 3D structures\|Cysteine desulfurase 3D structures]]
		+	*[[Selenocysteine lyase\|Selenocysteine lyase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dunkle~~, J A~~]]	+	[[Category: Dunkle JA]]
-	[[Category: Frantom~~, P A]]~~	+	[[Category: Frantom PA]]
-	~~[[Category: Cysteine desulfurase]]~~	+
-	~~[[Category: Persulfide]]~~	+
-	~~[[Category: Suf]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 11:28, 1 January 2020

OCA — Wed, 01 Jan 2020 11:28:16 GMT

←Older revision		Revision as of 11:28, 1 January 2020
Line 1:		Line 1:

	==E. coli cysteine desulfurase SufS R92A with a cysteine persulfide intermediate==		==E. coli cysteine desulfurase SufS R92A with a cysteine persulfide intermediate==
-	<StructureSection load='6mre' size='340' side='right' caption='[[6mre]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='6mre' size='340' side='right'caption='[[6mre]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6mre]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MRE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MRE FirstGlance]. <br>		<table><tr><td colspan='2'>[[6mre]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MRE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MRE FirstGlance]. <br>
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 6mre" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6mre" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cysteine desulfurase 3D structures\|Cysteine desulfurase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Dunkle, J A]]		[[Category: Dunkle, J A]]
	[[Category: Frantom, P A]]		[[Category: Frantom, P A]]

OCA at 16:07, 27 February 2019

OCA — Wed, 27 Feb 2019 16:07:00 GMT

←Older revision		Revision as of 16:07, 27 February 2019
Line 3:		Line 3:
	<StructureSection load='6mre' size='340' side='right' caption='[[6mre]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='6mre' size='340' side='right' caption='[[6mre]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mre]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MRE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MRE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mre]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MRE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MRE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6mr2\|6mr2]], [[6mr6\|6mr6]], [[6mrh\|6mrh]], [[6mri\|6mri]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6mr2\|6mr2]], [[6mr6\|6mr6]], [[6mrh\|6mrh]], [[6mri\|6mri]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sufS, csdB, ynhB, b1680, JW1670 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mre OCA], [http://pdbe.org/6mre PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mre RCSB], [http://www.ebi.ac.uk/pdbsum/6mre PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mre ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mre OCA], [http://pdbe.org/6mre PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mre RCSB], [http://www.ebi.ac.uk/pdbsum/6mre PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mre ProSAT]</span></td></tr>
	</table>		</table>
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Dunkle, J A]]		[[Category: Dunkle, J A]]
	[[Category: Frantom, P A]]		[[Category: Frantom, P A]]

OCA at 06:40, 9 January 2019

OCA — Wed, 09 Jan 2019 06:40:40 GMT

←Older revision		Revision as of 06:40, 9 January 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6mre is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==E. coli cysteine desulfurase SufS R92A with a cysteine persulfide intermediate==
		+	<StructureSection load='6mre' size='340' side='right' caption='[[6mre]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6mre]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MRE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MRE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6mr2\|6mr2]], [[6mr6\|6mr6]], [[6mrh\|6mrh]], [[6mri\|6mri]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mre OCA], [http://pdbe.org/6mre PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mre RCSB], [http://www.ebi.ac.uk/pdbsum/6mre PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mre ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SUFS_ECOLI SUFS_ECOLI]] Cysteine desulfurases mobilize the sulfur from L-cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Acts as a potent selenocysteine lyase in vitro, that mobilizes selenium from L-selenocysteine. Selenocysteine lyase activity is however unsure in vivo.<ref>PMID:10829016</ref> <ref>PMID:12089140</ref> <ref>PMID:11997471</ref> <ref>PMID:12876288</ref> <ref>PMID:12941942</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SufS is a type II cysteine desulfurase and acts as the initial step in the Suf Fe-S cluster assembly pathway. In Escherichia coli this pathway is utilized under conditions of oxidative stress and is resistant to reactive oxygen species. Mechanistically this means SufS must shift between protecting a covalent persulfide intermediate and making it available for transfer to the next protein partner in the pathway, SufE. Here, we report five x-ray crystal structures of SufS including a new structure of SufS containing an inward facing persulfide intermediate on C364. Additional structures of SufS variants with substitutions at the dimer interface show changes in dimer geometry and suggest a conserved beta-hairpin structure plays a role in mediating interactions with SufE. These new structures, along with previous HDX-MS and biochemical data identify an interaction network capable of communication between active-sites of the SufS dimer coordinating the shift between desulfurase and transpersulfurase activities.

-	~~Authors: Dunkle~~, ~~J.A~~., ~~Frantom~~, P.A.	+	Structural evidence for dimer-interface driven regulation of the type II cysteine desulfurase, SufS.,Dunkle JA, Bruno M, Outten FW, Frantom PA Biochemistry. 2018 Dec 20. doi: 10.1021/acs.biochem.8b01122. PMID:30571100<ref>PMID:30571100</ref>

-	~~Description: E. coli cysteine desulfurase SufS R92A with~~ a ~~cysteine persulfide intermediate~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Frantom, P.A]]	+	<div class="pdbe-citations 6mre" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Dunkle, J.A~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Dunkle, J A]]
		+	[[Category: Frantom, P A]]
		+	[[Category: Cysteine desulfurase]]
		+	[[Category: Persulfide]]
		+	[[Category: Suf]]
		+	[[Category: Transferase]]

OCA at 10:48, 7 November 2018

OCA — Wed, 07 Nov 2018 10:48:37 GMT

←Older revision		Revision as of 10:48, 7 November 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6mre is ON HOLD	+	The entry 6mre is ON HOLD until Paper Publication

	Authors: Dunkle, J.A., Frantom, P.A.		Authors: Dunkle, J.A., Frantom, P.A.

OCA: Protected "6mre" [edit=sysop:move=sysop]

OCA — Wed, 24 Oct 2018 04:30:54 GMT

Protected "6mre" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:30, 24 October 2018

OCA: New page: '''Unreleased structure''' The entry 6mre is ON HOLD Authors: Dunkle, J.A., Frantom, P.A. Description: E. coli cysteine desulfurase SufS R92A with a cysteine persulfide intermediate [[...

OCA — Wed, 24 Oct 2018 04:30:52 GMT

New page: '''Unreleased structure''' The entry 6mre is ON HOLD Authors: Dunkle, J.A., Frantom, P.A. Description: E. coli cysteine desulfurase SufS R92A with a cysteine persulfide intermediate [[...

New page

'''Unreleased structure'''

The entry 6mre is ON HOLD

Authors: Dunkle, J.A., Frantom, P.A.

Description: E. coli cysteine desulfurase SufS R92A with a cysteine persulfide intermediate
[[Category: Unreleased Structures]]
[[Category: Frantom, P.A]]
[[Category: Dunkle, J.A]]