6myj - Revision history

OCA at 06:41, 11 October 2023

OCA — Wed, 11 Oct 2023 06:41:49 GMT

←Older revision		Revision as of 06:41, 11 October 2023
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	<StructureSection load='6myj' size='340' side='right'caption='[[6myj]], [[Resolution\|resolution]] 1.33Å' scene=''>		<StructureSection load='6myj' size='340' side='right'caption='[[6myj]], [[Resolution\|resolution]] 1.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6myj]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Oyster_mushroom Oyster mushroom~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MYJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MYJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6myj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pleurotus_ostreatus Pleurotus ostreatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MYJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K6V:N-[(2S)-1-hydroxypropan-2-yl]butanamide'>K6V</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.33Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OlyA6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5322 Oyster mushroom])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K6V:N-[(2S)-1-hydroxypropan-2-yl]butanamide'>K6V</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6myj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6myj OCA], [~~http~~://pdbe.org/6myj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6myj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6myj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6myj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6myj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6myj OCA], [https://pdbe.org/6myj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6myj RCSB], [https://www.ebi.ac.uk/pdbsum/6myj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6myj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/OLYA6_PLEOS OLYA6_PLEOS]] Has hemolytic activity against bovine erythrocytes at nanomolar concentrations in vitro. Promotes active pleurotolysin B (PlyB)-dependent permeabilization of membranes rich in cholesterol and sphingomyelin. May play an important role in the initial phase of fungal fruiting.<ref>PMID:12020804</ref> <ref>PMID:23806422</ref>	+	[https://www.uniprot.org/uniprot/OLYA6_PLEOS OLYA6_PLEOS] Has hemolytic activity against bovine erythrocytes at nanomolar concentrations in vitro. Promotes active pleurotolysin B (PlyB)-dependent permeabilization of membranes rich in cholesterol and sphingomyelin. May play an important role in the initial phase of fungal fruiting.<ref>PMID:12020804</ref> <ref>PMID:23806422</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Oyster mushroom~~]]	+	[[Category: Pleurotus ostreatus]]
-	[[Category: Endapally, S]]	+	[[Category: Endapally S]]
-	[[Category: Radhakrishnan, A]]	+	[[Category: Radhakrishnan A]]
-	[[Category: Tomchick~~, D R]]~~	+	[[Category: Tomchick DR]]
-	~~[[Category: Beta-sandwich fold]]~~	+
-	~~[[Category: Membrane binding protein]]~~	+
-	~~[[Category: Membrane protein~~]]	+

OCA at 10:26, 4 December 2019

OCA — Wed, 04 Dec 2019 10:26:34 GMT

←Older revision		Revision as of 10:26, 4 December 2019
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	==Pleurotus ostreatus OstreolysinA plus sphingomyelin==		==Pleurotus ostreatus OstreolysinA plus sphingomyelin==
-	<StructureSection load='6myj' size='340' side='right' caption='[[6myj]], [[Resolution\|resolution]] 1.33Å' scene=''>	+	<StructureSection load='6myj' size='340' side='right'caption='[[6myj]], [[Resolution\|resolution]] 1.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6myj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Oyster_mushroom Oyster mushroom]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MYJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[6myj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Oyster_mushroom Oyster mushroom]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MYJ FirstGlance]. <br>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Oyster mushroom]]		[[Category: Oyster mushroom]]
	[[Category: Endapally, S]]		[[Category: Endapally, S]]

OCA at 08:31, 6 March 2019

OCA — Wed, 06 Mar 2019 08:31:01 GMT

←Older revision		Revision as of 08:31, 6 March 2019
Line 1:		Line 1:

	==Pleurotus ostreatus OstreolysinA plus sphingomyelin==		==Pleurotus ostreatus OstreolysinA plus sphingomyelin==
-	<StructureSection load='6myj' size='340' side='right' caption='[[6myj]], [[Resolution\|resolution]] 1.33Å' scene=''>	+	<StructureSection load='6myj' size='340' side='right' caption='[[6myj]], [[Resolution\|resolution]] 1.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6myj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MYJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6myj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Oyster_mushroom Oyster mushroom]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MYJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K6V:N-[(2S)-1-hydroxypropan-2-yl]butanamide'>K6V</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K6V:N-[(2S)-1-hydroxypropan-2-yl]butanamide'>K6V</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OlyA6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5322 Oyster mushroom])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6myj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6myj OCA], [http://pdbe.org/6myj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6myj RCSB], [http://www.ebi.ac.uk/pdbsum/6myj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6myj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6myj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6myj OCA], [http://pdbe.org/6myj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6myj RCSB], [http://www.ebi.ac.uk/pdbsum/6myj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6myj ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Oyster mushroom]]
	[[Category: Endapally, S]]		[[Category: Endapally, S]]
	[[Category: Radhakrishnan, A]]		[[Category: Radhakrishnan, A]]

OCA at 09:36, 13 February 2019

OCA — Wed, 13 Feb 2019 09:36:01 GMT

←Older revision		Revision as of 09:36, 13 February 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6myj is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Pleurotus ostreatus OstreolysinA plus sphingomyelin==
		+	<StructureSection load='6myj' size='340' side='right' caption='[[6myj]], [[Resolution\|resolution]] 1.33Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6myj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MYJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K6V:N-[(2S)-1-hydroxypropan-2-yl]butanamide'>K6V</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6myj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6myj OCA], [http://pdbe.org/6myj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6myj RCSB], [http://www.ebi.ac.uk/pdbsum/6myj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6myj ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/OLYA6_PLEOS OLYA6_PLEOS]] Has hemolytic activity against bovine erythrocytes at nanomolar concentrations in vitro. Promotes active pleurotolysin B (PlyB)-dependent permeabilization of membranes rich in cholesterol and sphingomyelin. May play an important role in the initial phase of fungal fruiting.<ref>PMID:12020804</ref> <ref>PMID:23806422</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sphingomyelin and cholesterol are essential lipids that are enriched in plasma membranes of animal cells, where they interact to regulate membrane properties and many intracellular signaling processes. Despite intense study, the interaction between these lipids in membranes is not well understood. Here, structural and biochemical analyses of ostreolysin A (OlyA), a protein that binds to membranes only when they contain both sphingomyelin and cholesterol, reveal that sphingomyelin adopts two distinct conformations in membranes when cholesterol is present. One conformation, bound by OlyA, is induced by stoichiometric, exothermic interactions with cholesterol, properties that are consistent with sphingomyelin/cholesterol complexes. In its second conformation, sphingomyelin is free from cholesterol and does not bind OlyA. A point mutation abolishes OlyA's ability to discriminate between these two conformations. In cells, levels of sphingomyelin/cholesterol complexes are held constant over a wide range of plasma membrane cholesterol concentrations, enabling precise regulation of the chemical activity of cholesterol.

-	~~Authors: Tomchick~~, D~~.R.~~, ~~Radhakrishnan~~, A., ~~Endapally~~, S.	+	Molecular Discrimination between Two Conformations of Sphingomyelin in Plasma Membranes.,Endapally S, Frias D, Grzemska M, Gay A, Tomchick DR, Radhakrishnan A Cell. 2019 Jan 25. pii: S0092-8674(18)31656-8. doi: 10.1016/j.cell.2018.12.042. PMID:30712872<ref>PMID:30712872</ref>

-	~~Description: Pleurotus ostreatus OstreolysinA plus sphingomyelin~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category: Tomchick~~, D.~~R]]~~	+	<div class="pdbe-citations 6myj" style="background-color:#fffaf0;"></div>
-	~~[[Category~~: ~~Radhakrishnan, A]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Endapally, S]]		[[Category: Endapally, S]]
		+	[[Category: Radhakrishnan, A]]
		+	[[Category: Tomchick, D R]]
		+	[[Category: Beta-sandwich fold]]
		+	[[Category: Membrane binding protein]]
		+	[[Category: Membrane protein]]

OCA at 06:39, 14 November 2018

OCA — Wed, 14 Nov 2018 06:39:53 GMT

←Older revision		Revision as of 06:39, 14 November 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6myj is ON HOLD	+	The entry 6myj is ON HOLD until Paper Publication

	Authors: Tomchick, D.R., Radhakrishnan, A., Endapally, S.		Authors: Tomchick, D.R., Radhakrishnan, A., Endapally, S.

-	Description: Pleurotus ostreatus OstreolysinA	+	Description: Pleurotus ostreatus OstreolysinA plus sphingomyelin
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Tomchick, D.R]]		[[Category: Tomchick, D.R]]
	[[Category: Radhakrishnan, A]]		[[Category: Radhakrishnan, A]]
	[[Category: Endapally, S]]		[[Category: Endapally, S]]

OCA: Protected "6myj" [edit=sysop:move=sysop]

OCA — Wed, 07 Nov 2018 10:59:33 GMT

Protected "6myj" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:59, 7 November 2018

OCA: New page: '''Unreleased structure''' The entry 6myj is ON HOLD Authors: Tomchick, D.R., Radhakrishnan, A., Endapally, S. Description: Pleurotus ostreatus OstreolysinA [[Category: Unreleased Stru...

OCA — Wed, 07 Nov 2018 10:59:32 GMT

New page: '''Unreleased structure''' The entry 6myj is ON HOLD Authors: Tomchick, D.R., Radhakrishnan, A., Endapally, S. Description: Pleurotus ostreatus OstreolysinA [[Category: Unreleased Stru...

New page

'''Unreleased structure'''

The entry 6myj is ON HOLD

Authors: Tomchick, D.R., Radhakrishnan, A., Endapally, S.

Description: Pleurotus ostreatus OstreolysinA
[[Category: Unreleased Structures]]
[[Category: Tomchick, D.R]]
[[Category: Radhakrishnan, A]]
[[Category: Endapally, S]]