6n1i - Revision history

OCA at 14:46, 13 March 2024

OCA — Wed, 13 Mar 2024 14:46:11 GMT

←Older revision		Revision as of 14:46, 13 March 2024
Line 3:		Line 3:
	<SX load='6n1i' size='340' side='right' viewer='molstar' caption='[[6n1i]], [[Resolution\|resolution]] 3.58Å' scene=''>		<SX load='6n1i' size='340' side='right' viewer='molstar' caption='[[6n1i]], [[Resolution\|resolution]] 3.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n1i]] is a 16 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6drp 6drp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6N1I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n1i]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6drp 6drp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N1I FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6n1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n1i OCA], [~~http~~://pdbe.org/6n1i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6n1i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6n1i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6n1i ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.58Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n1i OCA], [https://pdbe.org/6n1i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n1i RCSB], [https://www.ebi.ac.uk/pdbsum/6n1i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n1i ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN]] The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN] The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN]] Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).[UniProtKB:Q3UP24]<ref>PMID:15107016</ref>	+	[https://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN] Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).[UniProtKB:Q3UP24]<ref>PMID:15107016</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Canonical inflammasomes are cytosolic supramolecular complexes that activate caspase-1 upon sensing extrinsic microbial invasions and intrinsic sterile stress signals. During inflammasome assembly, adaptor proteins ASC and NLRC4 recruit caspase-1 through homotypic caspase recruitment domain (CARD) interactions, leading to caspase-1 dimerization and activation. Activated caspase-1 processes proinflammatory cytokines and Gasdermin D to induce cytokine maturation and pyroptotic cell death. Here, we present cryo-electron microscopy (cryo-EM) structures of NLRC4 CARD and ASC CARD filaments mediated by conserved three types of asymmetric interactions (types I, II, and III). We find that the CARDs of these two adaptor proteins share a similar assembly pattern, which matches that of the caspase-1 CARD filament whose structure we defined previously. These data indicate a unified mechanism for downstream caspase-1 recruitment through CARD-CARD interactions by both adaptors. Using structure modeling, we further show that full-length NLRC4 assembles via two separate symmetries at its CARD and its nucleotide-binding domain (NBD), respectively.	+
-		+
-	Cryo-EM structures of ASC and NLRC4 CARD filaments reveal a unified mechanism of nucleation and activation of caspase-1.,Li Y, Fu TM, Lu A, Witt K, Ruan J, Shen C, Wu H Proc Natl Acad Sci U S A. 2018 Oct 2. pii: 1810524115. doi:, 10.1073/pnas.1810524115. PMID:30279182<ref>PMID:30279182</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6n1i" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</SX>		</SX>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fu, T]]	+	[[Category: Fu T]]
-	[[Category: Li, Y]]	+	[[Category: Li Y]]
-	[[Category: Wu, H]]	+	[[Category: Wu H]]
-	~~[[Category: Filament]]~~	+
-	~~[[Category: Immunity]]~~	+
-	~~[[Category: Inflammasome]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 03:03, 11 April 2020

OCA — Sat, 11 Apr 2020 03:03:58 GMT

←Older revision		Revision as of 03:03, 11 April 2020
Line 3:		Line 3:
	<SX load='6n1i' size='340' side='right' viewer='molstar' caption='[[6n1i]], [[Resolution\|resolution]] 3.58Å' scene=''>		<SX load='6n1i' size='340' side='right' viewer='molstar' caption='[[6n1i]], [[Resolution\|resolution]] 3.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n1i]] is a 16 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6drp 6drp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1I OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6N1I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n1i]] is a 16 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6drp 6drp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1I OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6N1I FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6n1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n1i OCA], [http://pdbe.org/6n1i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n1i RCSB], [http://www.ebi.ac.uk/pdbsum/6n1i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n1i ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6n1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n1i OCA], [http://pdbe.org/6n1i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n1i RCSB], [http://www.ebi.ac.uk/pdbsum/6n1i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n1i ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==

OCA at 22:40, 6 March 2020

OCA — Fri, 06 Mar 2020 22:40:57 GMT

←Older revision		Revision as of 22:40, 6 March 2020
Line 1:		Line 1:

	==Cryo-EM structure of NLRC4-CARD filament==		==Cryo-EM structure of NLRC4-CARD filament==
-	<~~StructureSection~~ load='6n1i' size='340' side='right' caption='[[6n1i]], [[Resolution\|resolution]] 3.58Å' scene=''>	+	<SX load='6n1i' size='340' side='right' viewer='molstar' caption='[[6n1i]], [[Resolution\|resolution]] 3.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6n1i]] is a 16 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6drp 6drp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N1I FirstGlance]. <br>		<table><tr><td colspan='2'>[[6n1i]] is a 16 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6drp 6drp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N1I FirstGlance]. <br>
Line 22:		Line 22:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
		+	[[Category: Large Structures]]
	[[Category: Fu, T]]		[[Category: Fu, T]]
	[[Category: Li, Y]]		[[Category: Li, Y]]

OCA at 06:24, 5 December 2018

OCA — Wed, 05 Dec 2018 06:24:27 GMT

←Older revision		Revision as of 06:24, 5 December 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The~~ entry 6n1i is ~~ON HOLD~~	+	==Cryo-EM structure of NLRC4-CARD filament==
		+	<StructureSection load='6n1i' size='340' side='right' caption='[[6n1i]], [[Resolution\|resolution]] 3.58Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6n1i]] is a 16 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6drp 6drp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N1I FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n1i OCA], [http://pdbe.org/6n1i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n1i RCSB], [http://www.ebi.ac.uk/pdbsum/6n1i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n1i ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN]] The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN]] Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).[UniProtKB:Q3UP24]<ref>PMID:15107016</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Canonical inflammasomes are cytosolic supramolecular complexes that activate caspase-1 upon sensing extrinsic microbial invasions and intrinsic sterile stress signals. During inflammasome assembly, adaptor proteins ASC and NLRC4 recruit caspase-1 through homotypic caspase recruitment domain (CARD) interactions, leading to caspase-1 dimerization and activation. Activated caspase-1 processes proinflammatory cytokines and Gasdermin D to induce cytokine maturation and pyroptotic cell death. Here, we present cryo-electron microscopy (cryo-EM) structures of NLRC4 CARD and ASC CARD filaments mediated by conserved three types of asymmetric interactions (types I, II, and III). We find that the CARDs of these two adaptor proteins share a similar assembly pattern, which matches that of the caspase-1 CARD filament whose structure we defined previously. These data indicate a unified mechanism for downstream caspase-1 recruitment through CARD-CARD interactions by both adaptors. Using structure modeling, we further show that full-length NLRC4 assembles via two separate symmetries at its CARD and its nucleotide-binding domain (NBD), respectively.

-	~~Authors: Li~~, Y., Fu, T., Wu, H.	+	Cryo-EM structures of ASC and NLRC4 CARD filaments reveal a unified mechanism of nucleation and activation of caspase-1.,Li Y, Fu TM, Lu A, Witt K, Ruan J, Shen C, Wu H Proc Natl Acad Sci U S A. 2018 Oct 2. pii: 1810524115. doi:, 10.1073/pnas.1810524115. PMID:30279182<ref>PMID:30279182</ref>

-	~~Description: Cryo-EM structure~~ of ~~NLRC4-CARD filament~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 6n1i" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Fu, T]]		[[Category: Fu, T]]
	[[Category: Li, Y]]		[[Category: Li, Y]]
	[[Category: Wu, H]]		[[Category: Wu, H]]
		+	[[Category: Filament]]
		+	[[Category: Immunity]]
		+	[[Category: Inflammasome]]
		+	[[Category: Signaling protein]]

OCA: Protected "6n1i" [edit=sysop:move=sysop]

OCA — Wed, 14 Nov 2018 06:49:20 GMT

Protected "6n1i" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:49, 14 November 2018

OCA: New page: '''Unreleased structure''' The entry 6n1i is ON HOLD Authors: Li, Y., Fu, T., Wu, H. Description: Cryo-EM structure of NLRC4-CARD filament Category: Unreleased Structures [[Categor...

OCA — Wed, 14 Nov 2018 06:49:19 GMT

New page: '''Unreleased structure''' The entry 6n1i is ON HOLD Authors: Li, Y., Fu, T., Wu, H. Description: Cryo-EM structure of NLRC4-CARD filament Category: Unreleased Structures [[Categor...

New page

'''Unreleased structure'''

The entry 6n1i is ON HOLD

Authors: Li, Y., Fu, T., Wu, H.

Description: Cryo-EM structure of NLRC4-CARD filament
[[Category: Unreleased Structures]]
[[Category: Fu, T]]
[[Category: Li, Y]]
[[Category: Wu, H]]