6n3w - Revision history

OCA at 06:44, 11 October 2023

OCA — Wed, 11 Oct 2023 06:44:36 GMT

←Older revision		Revision as of 06:44, 11 October 2023
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	<StructureSection load='6n3w' size='340' side='right'caption='[[6n3w]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='6n3w' size='340' side='right'caption='[[6n3w]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n3w]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N3W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6N3W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n3w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N3W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N3W FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=KBJ:5-O~~-[~~(2-phenylethyl)carbamoyl~~]~~guanosine'>KBJ</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~HINT1, HINT, PKCI1, PRKCNH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id=9606 HUMAN]~~)</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KBJ:5-O-[(2-phenylethyl)carbamoyl]guanosine'>KBJ</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6n3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n3w OCA], [~~http~~://pdbe.org/6n3w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6n3w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6n3w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6n3w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n3w OCA], [https://pdbe.org/6n3w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n3w RCSB], [https://www.ebi.ac.uk/pdbsum/6n3w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n3w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HINT1_HUMAN HINT1_HUMAN]] Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2 (By similarity).	+	[https://www.uniprot.org/uniprot/HINT1_HUMAN HINT1_HUMAN] Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2 (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6n3w" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6n3w" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Histidine triad nucleotide-binding protein 3D structures\|Histidine triad nucleotide-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Finzel~~, B C~~]]	+	[[Category: Finzel BC]]
-	[[Category: Strom~~, A M]]~~	+	[[Category: Strom AM]]
-	~~[[Category: Hint]]~~	+
-	~~[[Category: Histidine triad]]~~	+
-	~~[[Category: Hit]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 09:35, 30 October 2019

OCA — Wed, 30 Oct 2019 09:35:42 GMT

←Older revision		Revision as of 09:35, 30 October 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6n3w is ~~ON HOLD until Paper~~ Publication	+	==Human Histidine Triad Nucleotide Binding Protein 1 (Hint1) with Bound 5'-O-[3-Phenyl-1-Ethyl]Carbamoyl Guanosine==
		+	<StructureSection load='6n3w' size='340' side='right'caption='[[6n3w]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6n3w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N3W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N3W FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=KBJ:5-O-[(2-phenylethyl)carbamoyl]guanosine'>KBJ</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HINT1, HINT, PKCI1, PRKCNH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n3w OCA], [http://pdbe.org/6n3w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n3w RCSB], [http://www.ebi.ac.uk/pdbsum/6n3w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n3w ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HINT1_HUMAN HINT1_HUMAN]] Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2 (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The interactions between the mu-opioid (MOR) and N-methyl-d-aspartate receptor (NMDAR) constitute an area of intense investigation because of their contributions to maladaptive neuroplasticity. Recent evidence suggests that their association requires the involvement of histidine triad nucleotide-binding protein (HINT1) with the enzyme's active site being critical in its regulatory role. Since it is known that spinal blockade of NMDA receptors prevents the development of opioid analgesic tolerance, we hypothesized that spinal inhibition of the HINT1 enzyme may similarly inhibit opioid tolerance. To address these questions, we evaluated novel HINT1 active-site inhibitors in two models of NMDAR and MOR interaction, namely, MOR inhibition of spinal NMDA activation and acute endomorphin-2 tolerance. These studies revealed that while the tryptamine carbamate of guanosine inhibitor, TrpGc, blocked both the development of opioid tolerance and the inhibitory effect of opioids on NMDA activation of the NMDA receptor, acyl-sulfamate analogues could only block the latter. Thermodynamic binding and X-ray crystallographic studies suggested that there are key differences between the bound HINT1-inhibitor surfaces that may be responsible for their differential ability to probe the ability of HINT1 to regulate cross talk between the mu-opioid receptor and NMDA receptor in the spinal cord.

-	~~Authors~~:	+	Inhibition of HINT1 Modulates Spinal Nociception and NMDA Evoked Behavior in Mice.,Shah RM, Peterson C, Strom A, Dillenburg M, Finzel B, Kitto KF, Fairbanks C, Wilcox G, Wagner CR ACS Chem Neurosci. 2019 Oct 16;10(10):4385-4393. doi:, 10.1021/acschemneuro.9b00432. Epub 2019 Sep 25. PMID:31503445<ref>PMID:31503445</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6n3w" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
		+	[[Category: Finzel, B C]]
		+	[[Category: Strom, A M]]
		+	[[Category: Hint]]
		+	[[Category: Histidine triad]]
		+	[[Category: Hit]]
		+	[[Category: Hydrolase]]

OCA at 05:19, 12 December 2018

OCA — Wed, 12 Dec 2018 05:19:29 GMT

←Older revision		Revision as of 05:19, 12 December 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6n3w is ON HOLD	+	The entry 6n3w is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6n3w" [edit=sysop:move=sysop]

OCA — Sun, 02 Dec 2018 19:14:00 GMT

Protected "6n3w" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:14, 2 December 2018

OCA: New page: '''Unreleased structure''' The entry 6n3w is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Sun, 02 Dec 2018 19:13:59 GMT

New page: '''Unreleased structure''' The entry 6n3w is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6n3w is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]