6n72 - Revision history

OCA at 06:47, 11 October 2023

OCA — Wed, 11 Oct 2023 06:47:18 GMT

←Older revision		Revision as of 06:47, 11 October 2023
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	<StructureSection load='6n72' size='340' side='right'caption='[[6n72]], [[Resolution\|resolution]] 2.73Å' scene=''>		<StructureSection load='6n72' size='340' side='right'caption='[[6n72]], [[Resolution\|resolution]] 2.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n72]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N72 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6N72 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n72]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N72 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>spaL, mxiB, spa47, CP0149 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~623~~ "~~Shigella paradysenteriae~~" ~~Weldin 1927])</td~~>~~</tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.735Å</td></tr>
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)~~-~~transporting_two-sector_ATPase H(+)-transporting two-sector ATPase]~~, ~~with EC number~~ [~~http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.1.~~2.~~2 7.1.2.2] </span>~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n72 OCA], [https://pdbe.org/6n72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n72 RCSB], [https://www.ebi.ac.uk/pdbsum/6n72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n72 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6n72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n72 OCA], [~~http~~://pdbe.org/6n72 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6n72 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6n72 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6n72 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~SPAL_SHIFL SPAL_SHIFL~~]~~] Required for surface presentation~~ of ~~invasion plasmid antigens~~. ~~Probable catalytic subunit of~~ a ~~protein translocase~~. Required for invasion and ~~for secretion~~ of the ~~three IPA proteins~~.	+	[https://www.uniprot.org/uniprot/SCTN_SHIFL SCTN_SHIFL] ATPase component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:26947936, PubMed:27770024, PubMed:29595954). Acts as a molecular motor to provide the energy that is required for the export of proteins (Probable). Required for type III secretion apparatus (T3SA) formation, proper protein secretion, host cell invasion and virulence (PubMed:26947936, PubMed:27770024, PubMed:31162724). May play a critical role in T3SS substrate recognition, disassembly of the effector/chaperone complex and unfolding of the effector in an ATP-dependent manner prior to secretion (By similarity).[UniProtKB:P0A1B9]<ref>PMID:26947936</ref> <ref>PMID:27770024</ref> <ref>PMID:29595954</ref> <ref>PMID:31162724</ref> <ref>PMID:26947936</ref> <ref>PMID:27770024</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6n72" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6n72" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[ATPase 3D structures\|ATPase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Shigella paradysenteriae weldin 1927]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Demler, H J~~]]	+	[[Category: Shigella flexneri]]
-	[[Category: ~~Dickenson, N E~~]]	+	[[Category: Demler HJ]]
-	[[Category: ~~Johnson, S J~~]]	+	[[Category: Dickenson NE]]
-	[[Category: ~~Morales, Y~~]]	+	[[Category: Johnson SJ]]
-	[[Category: ~~Atpase]]~~	+	[[Category: Morales Y]]
-	~~[[Category: Biosynthetic protein~~]]	+

OCA at 07:59, 23 October 2019

OCA — Wed, 23 Oct 2019 07:59:08 GMT

←Older revision		Revision as of 07:59, 23 October 2019
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	<StructureSection load='6n72' size='340' side='right'caption='[[6n72]], [[Resolution\|resolution]] 2.73Å' scene=''>		<StructureSection load='6n72' size='340' side='right'caption='[[6n72]], [[Resolution\|resolution]] 2.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n72]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N72 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n72]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N72 FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.1.2.2 7.1.2.2] </span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spaL, mxiB, spa47, CP0149 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.1.2.2 7.1.2.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n72 OCA], [http://pdbe.org/6n72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n72 RCSB], [http://www.ebi.ac.uk/pdbsum/6n72 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n72 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n72 OCA], [http://pdbe.org/6n72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n72 RCSB], [http://www.ebi.ac.uk/pdbsum/6n72 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n72 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Shigella paradysenteriae weldin 1927]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Demler, H J]]		[[Category: Demler, H J]]

OCA at 07:32, 26 June 2019

OCA — Wed, 26 Jun 2019 07:32:47 GMT

←Older revision		Revision as of 07:32, 26 June 2019
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SPAL_SHIFL SPAL_SHIFL]] Required for surface presentation of invasion plasmid antigens. Probable catalytic subunit of a protein translocase. Required for invasion and for secretion of the three IPA proteins.		[[http://www.uniprot.org/uniprot/SPAL_SHIFL SPAL_SHIFL]] Required for surface presentation of invasion plasmid antigens. Probable catalytic subunit of a protein translocase. Required for invasion and for secretion of the three IPA proteins.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Like many Gram-negative pathogens, Shigella rely on a type three secretion system (T3SS) for injection of effector proteins directly into eukaryotic host cells to initiate and sustain infection. Protein secretion through the needle-like type three secretion apparatus (T3SA) requires ATP hydrolysis by the T3SS ATPase Spa47, making it a likely target for in vivo regulation of T3SS activity and an attractive target for small molecule therapeutics against shigellosis. Here, we developed a model of an activated Spa47 homo-hexamer, identifying two distinct regions at each protomer interface that we hypothesized to provide intermolecular interactions supporting Spa47 oligomerization and enzymatic activation. Mutational analysis and a series of high-resolution crystal structures confirm the importance of these residues, as many of the engineered mutants are unable to form oligomers and efficiently hydrolyze ATP in vitro. Furthermore, in vivo evaluation of Shigella virulence phenotype uncovered a strong correlation between T3SS effector protein secretion, host cell membrane disruption, and cellular invasion by the tested mutant strains, suggesting that perturbation of the identified interfacial residues/interactions influences Spa47 activity through preventing oligomer formation, which in turn regulates Shigella virulence. The most impactful mutations are observed within the conserved Site 2 interface where the native residues support oligomerization and likely contribute to a complex hydrogen bonding network that organizes the active site and supports catalysis. The critical reliance on these conserved residues suggests that aspects of T3SS regulation may also be conserved, providing promise for the development of a cross-species therapeutic that broadly targets T3SS ATPase oligomerization and activation.
		+
		+	Interfacial amino acids support Spa47 oligomerization and shigella type three secretion system activation.,Demler HJ, Case HB, Morales Y, Bernard AR, Johnson SJ, Dickenson NE Proteins. 2019 Jun 4. doi: 10.1002/prot.25754. PMID:31162724<ref>PMID:31162724</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6n72" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:01, 19 June 2019

OCA — Wed, 19 Jun 2019 06:01:08 GMT

←Older revision		Revision as of 06:01, 19 June 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6n72 is ~~ON HOLD until Paper Publication~~	+	==Crystal Structure of ATPase delta1-79 Spa47 E267R==
-		+	<StructureSection load='6n72' size='340' side='right'caption='[[6n72]], [[Resolution\|resolution]] 2.73Å' scene=''>
-	~~Authors~~: ~~Morales~~, Y., ~~Johnson~~, S.J., ~~Demler, H~~.J., ~~Dickenson, N~~.E.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6n72]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N72 FirstGlance]. <br>
-	~~Description~~: ~~Crystal Structure~~ of ~~ATPase delta1-79 Spa47 E267R~~	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.1.2.2 7.1.2.2] </span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n72 OCA], [http://pdbe.org/6n72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n72 RCSB], [http://www.ebi.ac.uk/pdbsum/6n72 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n72 ProSAT]</span></td></tr>
-	[[Category: Dickenson, N.E]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPAL_SHIFL SPAL_SHIFL]] Required for surface presentation of invasion plasmid antigens. Probable catalytic subunit of a protein translocase. Required for invasion and for secretion of the three IPA proteins.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Demler, H J]]
		+	[[Category: Dickenson, N E]]
		+	[[Category: Johnson, S J]]
	[[Category: Morales, Y]]		[[Category: Morales, Y]]
-	[[Category: ~~Demler, H.J~~]]	+	[[Category: Atpase]]
-	[[Category: ~~Johnson, S.J~~]]	+	[[Category: Biosynthetic protein]]

OCA at 06:26, 30 January 2019

OCA — Wed, 30 Jan 2019 06:26:30 GMT

←Older revision		Revision as of 06:26, 30 January 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6n72 is ON HOLD	+	The entry 6n72 is ON HOLD until Paper Publication

	Authors: Morales, Y., Johnson, S.J., Demler, H.J., Dickenson, N.E.		Authors: Morales, Y., Johnson, S.J., Demler, H.J., Dickenson, N.E.
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	[[Category: Dickenson, N.E]]		[[Category: Dickenson, N.E]]
	[[Category: Morales, Y]]		[[Category: Morales, Y]]
-	[[Category: Johnson, S.J]]
	[[Category: Demler, H.J]]		[[Category: Demler, H.J]]
		+	[[Category: Johnson, S.J]]

OCA: Protected "6n72" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2018 05:18:17 GMT

Protected "6n72" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:18, 5 December 2018

OCA: New page: '''Unreleased structure''' The entry 6n72 is ON HOLD Authors: Morales, Y., Johnson, S.J., Demler, H.J., Dickenson, N.E. Description: Crystal Structure of ATPase delta1-79 Spa47 E267R [...

OCA — Wed, 05 Dec 2018 05:18:16 GMT

New page: '''Unreleased structure''' The entry 6n72 is ON HOLD Authors: Morales, Y., Johnson, S.J., Demler, H.J., Dickenson, N.E. Description: Crystal Structure of ATPase delta1-79 Spa47 E267R [...

New page

'''Unreleased structure'''

The entry 6n72 is ON HOLD

Authors: Morales, Y., Johnson, S.J., Demler, H.J., Dickenson, N.E.

Description: Crystal Structure of ATPase delta1-79 Spa47 E267R
[[Category: Unreleased Structures]]
[[Category: Dickenson, N.E]]
[[Category: Morales, Y]]
[[Category: Johnson, S.J]]
[[Category: Demler, H.J]]