6n7o - Revision history

OCA at 14:46, 13 March 2024

OCA — Wed, 13 Mar 2024 14:46:50 GMT

←Older revision		Revision as of 14:46, 13 March 2024
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	<StructureSection load='6n7o' size='340' side='right'caption='[[6n7o]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='6n7o' size='340' side='right'caption='[[6n7o]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n7o]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacillus_phage_pgil01~~ Bacillus phage ~~pgil01~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7O OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6N7O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n7o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_phage_pGIL01 Bacillus phage pGIL01]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N7O FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n7o OCA], [~~http~~://pdbe.org/6n7o PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6n7o RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6n7o PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6n7o ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n7o OCA], [https://pdbe.org/6n7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n7o RCSB], [https://www.ebi.ac.uk/pdbsum/6n7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n7o ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q7WSG2_9VIRU Q7WSG2_9VIRU]
-	Bacteria identify and respond to DNA damage using the SOS response. LexA, a central repressor in the response, has been implicated in the regulation of lysogeny in various temperate bacteriophages. During infection of Bacillus thuringiensis with GIL01 bacteriophage, LexA represses the SOS response and the phage lytic cycle by binding DNA, an interaction further stabilized upon binding of a viral protein, gp7. Here we report the crystallographic structure of phage-borne gp7 at 1.7-A resolution, and characterize the 4:2 stoichiometry and potential interaction with LexA using surface plasmon resonance, static light scattering, and small-angle X-ray scattering. These data suggest that gp7 stabilizes LexA binding to operator DNA via coordination of the N- and C-terminal domains of LexA. Furthermore, we have found that gp7 can interact with LexA from Staphylococcus aureus, a significant human pathogen. Our results provide structural evidence as to how phage factors can directly associate with LexA to modulate the SOS response.	+
-		+
-	Structural Insights into Bacteriophage GIL01 gp7 Inhibition of Host LexA Repressor.,Caveney NA, Pavlin A, Caballero G, Bahun M, Hodnik V, de Castro L, Fornelos N, Butala M, Strynadka NCJ Structure. 2019 Apr 10. pii: S0969-2126(19)30115-7. doi:, 10.1016/~~j.str.2019.03.019. PMID:31056420<ref>PMID:31056420<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6n7o" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus phage ~~pgil01~~]]	+	[[Category: Bacillus phage pGIL01]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Caveney~~, N A~~]]	+	[[Category: Caveney NA]]
-	[[Category: Strynadka~~, N C.J]]~~	+	[[Category: Strynadka NCJ]]
-	~~[[Category: Viral protein~~]]	+

OCA at 07:33, 21 May 2019

OCA — Tue, 21 May 2019 07:33:07 GMT

←Older revision		Revision as of 07:33, 21 May 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6n7o is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of GIL01 gp7==
		+	<StructureSection load='6n7o' size='340' side='right'caption='[[6n7o]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6n7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_pgil01 Bacillus phage pgil01]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N7O FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n7o OCA], [http://pdbe.org/6n7o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n7o RCSB], [http://www.ebi.ac.uk/pdbsum/6n7o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n7o ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteria identify and respond to DNA damage using the SOS response. LexA, a central repressor in the response, has been implicated in the regulation of lysogeny in various temperate bacteriophages. During infection of Bacillus thuringiensis with GIL01 bacteriophage, LexA represses the SOS response and the phage lytic cycle by binding DNA, an interaction further stabilized upon binding of a viral protein, gp7. Here we report the crystallographic structure of phage-borne gp7 at 1.7-A resolution, and characterize the 4:2 stoichiometry and potential interaction with LexA using surface plasmon resonance, static light scattering, and small-angle X-ray scattering. These data suggest that gp7 stabilizes LexA binding to operator DNA via coordination of the N- and C-terminal domains of LexA. Furthermore, we have found that gp7 can interact with LexA from Staphylococcus aureus, a significant human pathogen. Our results provide structural evidence as to how phage factors can directly associate with LexA to modulate the SOS response.

-	~~Authors~~:	+	Structural Insights into Bacteriophage GIL01 gp7 Inhibition of Host LexA Repressor.,Caveney NA, Pavlin A, Caballero G, Bahun M, Hodnik V, de Castro L, Fornelos N, Butala M, Strynadka NCJ Structure. 2019 Apr 10. pii: S0969-2126(19)30115-7. doi:, 10.1016/j.str.2019.03.019. PMID:31056420<ref>PMID:31056420</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6n7o" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bacillus phage pgil01]]
		+	[[Category: Large Structures]]
		+	[[Category: Caveney, N A]]
		+	[[Category: Strynadka, N C.J]]
		+	[[Category: Viral protein]]

OCA at 06:27, 30 January 2019

OCA — Wed, 30 Jan 2019 06:27:09 GMT

←Older revision		Revision as of 06:27, 30 January 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6n7o is ON HOLD	+	The entry 6n7o is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6n7o" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2018 05:19:34 GMT

Protected "6n7o" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:19, 5 December 2018

OCA: New page: '''Unreleased structure''' The entry 6n7o is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 05 Dec 2018 05:19:33 GMT

New page: '''Unreleased structure''' The entry 6n7o is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6n7o is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]