6n7w - Revision history

OCA at 09:20, 20 March 2024

OCA — Wed, 20 Mar 2024 09:20:48 GMT

←Older revision		Revision as of 09:20, 20 March 2024
Line 3:		Line 3:
	<SX load='6n7w' size='340' side='right' viewer='molstar' caption='[[6n7w]], [[Resolution\|resolution]] 4.50Å' scene=''>		<SX load='6n7w' size='340' side='right' viewer='molstar' caption='[[6n7w]], [[Resolution\|resolution]] 4.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n7w]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~] and [~~http~~://en.wikipedia.org/wiki/~~Bpt7 Bpt7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6N7W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n7w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N7W FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~, ~~<scene name='pdbligand=TTP:THYMIDINE-~~5~~-TRIPHOSPHATE'>TTP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.5Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=2DA:2,3-DIDEOXYADENOSINE-5-MONOPHOSPHATE'>2DA</scene><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2DA:2,3-DIDEOXYADENOSINE-5-MONOPHOSPHATE'>2DA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">trxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n7w OCA], [https://pdbe.org/6n7w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n7w RCSB], [https://www.ebi.ac.uk/pdbsum/6n7w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n7w ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6n7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n7w OCA], [~~http~~://pdbe.org/6n7w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6n7w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6n7w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6n7w ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/DPOL_BPT7 DPOL_BPT7]] Replicates viral genomic DNA. Non-processive DNA polymerase that achieves processivity by binding to host thioredoxin (TrxA). This interaction increases the rate of dNTP incorporation to yield a processivity of approximately 800 nucleotides (nt) per binding event. Interacts with DNA helicase gp4 to coordinate nucleotide polymerization with unwinding of the DNA. The leading strand is synthesized continuously while synthesis of the lagging strand requires the synthesis of oligoribonucleotides by the primase domain of gp4.<ref>PMID:9218486</ref> <ref>PMID:21606333</ref>	+	[https://www.uniprot.org/uniprot/DPOL_BPT7 DPOL_BPT7] Replicates viral genomic DNA. Non-processive DNA polymerase that achieves processivity by binding to host thioredoxin (TrxA). This interaction increases the rate of dNTP incorporation to yield a processivity of approximately 800 nucleotides (nt) per binding event. Interacts with DNA helicase gp4 to coordinate nucleotide polymerization with unwinding of the DNA. The leading strand is synthesized continuously while synthesis of the lagging strand requires the synthesis of oligoribonucleotides by the primase domain of gp4.<ref>PMID:9218486</ref> <ref>PMID:21606333</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Visualization in atomic detail of the replisome that performs concerted leading- and lagging-DNA strand synthesis at a replication fork has not been reported. Using bacteriophage T7 as a model system, we determined cryo-electron microscopy structures up to 3.2-angstroms resolution of helicase translocating along DNA and of helicase-polymerase-primase complexes engaging in synthesis of both DNA strands. Each domain of the spiral-shaped hexameric helicase translocates sequentially hand-over-hand along a single-stranded DNA coil, akin to the way AAA+ ATPases (adenosine triphosphatases) unfold peptides. Two lagging-strand polymerases are attached to the primase, ready for Okazaki fragment synthesis in tandem. A beta hairpin from the leading-strand polymerase separates two parental DNA strands into a T-shaped fork, thus enabling the closely coupled helicase to advance perpendicular to the downstream DNA duplex. These structures reveal the molecular organization and operating principles of a replisome.	+
-		+
-	Structures and operating principles of the replisome.,Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W Science. 2019 Feb 22;363(6429). pii: science.aav7003. doi:, 10.1126/science.aav7003. Epub 2019 Jan 24. PMID:30679383<ref>PMID:30679383</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6n7w" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
		+	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
	*[[Thioredoxin 3D structures\|Thioredoxin 3D structures]]		*[[Thioredoxin 3D structures\|Thioredoxin 3D structures]]
	== References ==		== References ==
Line 27:		Line 18:
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
-	[[Category: ~~Bpt7~~]]	+	[[Category: Escherichia phage T7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fox, T]]	+	[[Category: Fox T]]
-	[[Category: Gao, Y]]	+	[[Category: Gao Y]]
-	[[Category: Val, N]]	+	[[Category: Val N]]
-	[[Category: Yang, W]]	+	[[Category: Yang W]]
-	~~[[Category: Dna polymerase]]~~	+
-	~~[[Category: Dna replication]]~~	+
-	~~[[Category: Helicase]]~~	+
-	~~[[Category: Replisome]]~~	+
-	~~[[Category: Transferase-dna complex~~]]	+

OCA at 03:11, 11 April 2020

OCA — Sat, 11 Apr 2020 03:11:33 GMT

←Older revision		Revision as of 03:11, 11 April 2020
Line 3:		Line 3:
	<SX load='6n7w' size='340' side='right' viewer='molstar' caption='[[6n7w]], [[Resolution\|resolution]] 4.50Å' scene=''>		<SX load='6n7w' size='340' side='right' viewer='molstar' caption='[[6n7w]], [[Resolution\|resolution]] 4.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n7w]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7W OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6N7W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n7w]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6N7W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=2DA:2,3-DIDEOXYADENOSINE-5-MONOPHOSPHATE'>2DA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=2DA:2,3-DIDEOXYADENOSINE-5-MONOPHOSPHATE'>2DA</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">trxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">trxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6n7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n7w OCA], [http://pdbe.org/6n7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n7w RCSB], [http://www.ebi.ac.uk/pdbsum/6n7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n7w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6n7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n7w OCA], [http://pdbe.org/6n7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n7w RCSB], [http://www.ebi.ac.uk/pdbsum/6n7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n7w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 22:47, 6 March 2020

OCA — Fri, 06 Mar 2020 22:47:05 GMT

←Older revision		Revision as of 22:47, 6 March 2020
Line 1:		Line 1:

	==Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx in complex with a DNA fork and incoming dTTP (from multiple lead complexes)==		==Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx in complex with a DNA fork and incoming dTTP (from multiple lead complexes)==
-	<~~StructureSection~~ load='6n7w' size='340' side='right'caption='[[6n7w]], [[Resolution\|resolution]] 4.50Å' scene=''>	+	<SX load='6n7w' size='340' side='right' viewer='molstar' caption='[[6n7w]], [[Resolution\|resolution]] 4.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6n7w]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N7W FirstGlance]. <br>		<table><tr><td colspan='2'>[[6n7w]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N7W FirstGlance]. <br>
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 6n7w" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6n7w" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Thioredoxin 3D structures\|Thioredoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Bpt7]]		[[Category: Bpt7]]

OCA at 11:21, 18 December 2019

OCA — Wed, 18 Dec 2019 11:21:45 GMT

←Older revision		Revision as of 11:21, 18 December 2019
Line 1:		Line 1:

	==Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx in complex with a DNA fork and incoming dTTP (from multiple lead complexes)==		==Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx in complex with a DNA fork and incoming dTTP (from multiple lead complexes)==
-	<StructureSection load='6n7w' size='340' side='right' caption='[[6n7w]], [[Resolution\|resolution]] 4.50Å' scene=''>	+	<StructureSection load='6n7w' size='340' side='right'caption='[[6n7w]], [[Resolution\|resolution]] 4.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n7w]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N7W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n7w]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N7W FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=2DA:2,3-DIDEOXYADENOSINE-5-MONOPHOSPHATE'>2DA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=2DA:2,3-DIDEOXYADENOSINE-5-MONOPHOSPHATE'>2DA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">trxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n7w OCA], [http://pdbe.org/6n7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n7w RCSB], [http://www.ebi.ac.uk/pdbsum/6n7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n7w ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n7w OCA], [http://pdbe.org/6n7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n7w RCSB], [http://www.ebi.ac.uk/pdbsum/6n7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n7w ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
		+	[[Category: Bpt7]]
		+	[[Category: Large Structures]]
	[[Category: Fox, T]]		[[Category: Fox, T]]
	[[Category: Gao, Y]]		[[Category: Gao, Y]]

OCA at 07:34, 6 March 2019

OCA — Wed, 06 Mar 2019 07:34:41 GMT

←Older revision		Revision as of 07:34, 6 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6n7w is ~~ON HOLD~~	+	==Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx in complex with a DNA fork and incoming dTTP (from multiple lead complexes)==
		+	<StructureSection load='6n7w' size='340' side='right' caption='[[6n7w]], [[Resolution\|resolution]] 4.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6n7w]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N7W FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=2DA:2,3-DIDEOXYADENOSINE-5-MONOPHOSPHATE'>2DA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n7w OCA], [http://pdbe.org/6n7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n7w RCSB], [http://www.ebi.ac.uk/pdbsum/6n7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n7w ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DPOL_BPT7 DPOL_BPT7]] Replicates viral genomic DNA. Non-processive DNA polymerase that achieves processivity by binding to host thioredoxin (TrxA). This interaction increases the rate of dNTP incorporation to yield a processivity of approximately 800 nucleotides (nt) per binding event. Interacts with DNA helicase gp4 to coordinate nucleotide polymerization with unwinding of the DNA. The leading strand is synthesized continuously while synthesis of the lagging strand requires the synthesis of oligoribonucleotides by the primase domain of gp4.<ref>PMID:9218486</ref> <ref>PMID:21606333</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Visualization in atomic detail of the replisome that performs concerted leading- and lagging-DNA strand synthesis at a replication fork has not been reported. Using bacteriophage T7 as a model system, we determined cryo-electron microscopy structures up to 3.2-angstroms resolution of helicase translocating along DNA and of helicase-polymerase-primase complexes engaging in synthesis of both DNA strands. Each domain of the spiral-shaped hexameric helicase translocates sequentially hand-over-hand along a single-stranded DNA coil, akin to the way AAA+ ATPases (adenosine triphosphatases) unfold peptides. Two lagging-strand polymerases are attached to the primase, ready for Okazaki fragment synthesis in tandem. A beta hairpin from the leading-strand polymerase separates two parental DNA strands into a T-shaped fork, thus enabling the closely coupled helicase to advance perpendicular to the downstream DNA duplex. These structures reveal the molecular organization and operating principles of a replisome.

-	~~Authors:~~ Gao, Y., Fox, T., ~~Val~~, N., ~~Yang~~, W.	+	Structures and operating principles of the replisome.,Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W Science. 2019 Feb 22;363(6429). pii: science.aav7003. doi:, 10.1126/science.aav7003. Epub 2019 Jan 24. PMID:30679383<ref>PMID:30679383</ref>

-	~~Description: Structure of bacteriophage T7 leading-strand DNA polymerase (D5A~~/~~E7A)/Trx and gp4 in complex with~~ a ~~DNA fork and incoming dTTP (from gp4~~-~~gp5 leading~~-~~strand complex)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6n7w" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Fox, T]]
	[[Category: Gao, Y]]		[[Category: Gao, Y]]
	[[Category: Val, N]]		[[Category: Val, N]]
-	[[Category: Fox, T]]
	[[Category: Yang, W]]		[[Category: Yang, W]]
		+	[[Category: Dna polymerase]]
		+	[[Category: Dna replication]]
		+	[[Category: Helicase]]
		+	[[Category: Replisome]]
		+	[[Category: Transferase-dna complex]]

OCA at 13:41, 27 February 2019

OCA — Wed, 27 Feb 2019 13:41:19 GMT

←Older revision		Revision as of 13:41, 27 February 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6n7w is ON HOLD ~~until Paper Publication~~	+	The entry 6n7w is ON HOLD

	Authors: Gao, Y., Fox, T., Val, N., Yang, W.		Authors: Gao, Y., Fox, T., Val, N., Yang, W.

-	Description: ~~D5AE7A mutant gp5~~ DNA polymerase ~~complexed~~ with ~~trx, fork~~ DNA, and incoming dTTP (from gp4-gp5 leading-strand ~~complexes~~)	+	Description: Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx and gp4 in complex with a DNA fork and incoming dTTP (from gp4-gp5 leading-strand complex)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Gao, Y]]		[[Category: Gao, Y]]

OCA at 05:02, 21 February 2019

OCA — Thu, 21 Feb 2019 05:02:44 GMT

←Older revision		Revision as of 05:02, 21 February 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6n7w is ON HOLD	+	The entry 6n7w is ON HOLD until Paper Publication

	Authors: Gao, Y., Fox, T., Val, N., Yang, W.		Authors: Gao, Y., Fox, T., Val, N., Yang, W.

OCA: Protected "6n7w" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2018 05:20:08 GMT

Protected "6n7w" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:20, 5 December 2018

OCA: New page: '''Unreleased structure''' The entry 6n7w is ON HOLD Authors: Gao, Y., Fox, T., Val, N., Yang, W. Description: D5AE7A mutant gp5 DNA polymerase complexed with trx, fork DNA, and incomi...

OCA — Wed, 05 Dec 2018 05:20:07 GMT

New page: '''Unreleased structure''' The entry 6n7w is ON HOLD Authors: Gao, Y., Fox, T., Val, N., Yang, W. Description: D5AE7A mutant gp5 DNA polymerase complexed with trx, fork DNA, and incomi...

New page

'''Unreleased structure'''

The entry 6n7w is ON HOLD

Authors: Gao, Y., Fox, T., Val, N., Yang, W.

Description: D5AE7A mutant gp5 DNA polymerase complexed with trx, fork DNA, and incoming dTTP (from gp4-gp5 leading-strand complexes)
[[Category: Unreleased Structures]]
[[Category: Gao, Y]]
[[Category: Val, N]]
[[Category: Fox, T]]
[[Category: Yang, W]]