6n86 - Revision history

OCA at 14:46, 13 March 2024

OCA — Wed, 13 Mar 2024 14:46:53 GMT

←Older revision		Revision as of 14:46, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6n86' size='340' side='right'caption='[[6n86]], [[Resolution\|resolution]] 3.90Å' scene=''>		<StructureSection load='6n86' size='340' side='right'caption='[[6n86]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n86]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N86 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6N86 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n86]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N86 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N86 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~RIC8A (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9913 BOVIN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.9Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6n86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n86 OCA], [~~http~~://pdbe.org/6n86 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6n86 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6n86 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6n86 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n86 OCA], [https://pdbe.org/6n86 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n86 RCSB], [https://www.ebi.ac.uk/pdbsum/6n86 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n86 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RIC8A_BOVIN RIC8A_BOVIN]] Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation (By similarity).	+	[https://www.uniprot.org/uniprot/RIC8A_BOVIN RIC8A_BOVIN] Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation (By similarity).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Resistance to inhibitors of cholinesterase 8A (Ric8A) is an essential regulator of G protein alpha-subunits (Galpha), acting as a guanine nucleotide exchange factor and a chaperone. We report two crystal structures of Ric8A, one in the apo form and the other in complex with a tagged C-terminal fragment of Galpha. These structures reveal two principal domains of Ric8A: an armadillo-fold core and a flexible C-terminal tail. Additionally, they show that the Galpha C-terminus binds to a highly-conserved patch on the concave surface of the Ric8A armadillo-domain, with selectivity determinants residing in the Galpha sequence. Biochemical analysis shows that the Ric8A C-terminal tail is critical for its stability and function. A model of the Ric8A/Galpha complex derived from crosslinking mass spectrometry and molecular dynamics simulations suggests that the Ric8A C-terminal tail helps organize the GTP-binding site of Galpha. This study lays the groundwork for understanding Ric8A function at the molecular level.	+
-		+
-	Structural underpinnings of Ric8A function as a G-protein alpha-subunit chaperone and guanine-nucleotide exchange factor.,Srivastava D, Gakhar L, Artemyev NO Nat Commun. 2019 Jul 12;10(1):3084. doi: 10.1038/s41467-019-11088-x. PMID:31300652<ref>PMID:31300652</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6n86" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bovin~~]]	+	[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Artemyev~~, N O~~]]	+	[[Category: Artemyev NO]]
-	[[Category: Gakhar, L]]	+	[[Category: Gakhar L]]
-	[[Category: Srivastava, D]]	+	[[Category: Srivastava D]]
-	~~[[Category: Armadillo repeat]]~~	+
-	~~[[Category: Chaperone]]~~	+
-	~~[[Category: G alpha]]~~	+
-	~~[[Category: Ric8a~~]]	+

OCA at 08:39, 24 July 2019

OCA — Wed, 24 Jul 2019 08:39:21 GMT

←Older revision		Revision as of 08:39, 24 July 2019
Line 3:		Line 3:
	<StructureSection load='6n86' size='340' side='right'caption='[[6n86]], [[Resolution\|resolution]] 3.90Å' scene=''>		<StructureSection load='6n86' size='340' side='right'caption='[[6n86]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n86]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N86 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N86 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n86]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N86 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N86 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n86 OCA], [http://pdbe.org/6n86 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n86 RCSB], [http://www.ebi.ac.uk/pdbsum/6n86 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n86 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RIC8A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n86 OCA], [http://pdbe.org/6n86 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n86 RCSB], [http://www.ebi.ac.uk/pdbsum/6n86 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n86 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/RIC8A_BOVIN RIC8A_BOVIN]] Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation (By similarity).		[[http://www.uniprot.org/uniprot/RIC8A_BOVIN RIC8A_BOVIN]] Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Resistance to inhibitors of cholinesterase 8A (Ric8A) is an essential regulator of G protein alpha-subunits (Galpha), acting as a guanine nucleotide exchange factor and a chaperone. We report two crystal structures of Ric8A, one in the apo form and the other in complex with a tagged C-terminal fragment of Galpha. These structures reveal two principal domains of Ric8A: an armadillo-fold core and a flexible C-terminal tail. Additionally, they show that the Galpha C-terminus binds to a highly-conserved patch on the concave surface of the Ric8A armadillo-domain, with selectivity determinants residing in the Galpha sequence. Biochemical analysis shows that the Ric8A C-terminal tail is critical for its stability and function. A model of the Ric8A/Galpha complex derived from crosslinking mass spectrometry and molecular dynamics simulations suggests that the Ric8A C-terminal tail helps organize the GTP-binding site of Galpha. This study lays the groundwork for understanding Ric8A function at the molecular level.
		+
		+	Structural underpinnings of Ric8A function as a G-protein alpha-subunit chaperone and guanine-nucleotide exchange factor.,Srivastava D, Gakhar L, Artemyev NO Nat Commun. 2019 Jul 12;10(1):3084. doi: 10.1038/s41467-019-11088-x. PMID:31300652<ref>PMID:31300652</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6n86" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bovin]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Artemyev, N O]]		[[Category: Artemyev, N O]]

OCA at 05:46, 10 July 2019

OCA — Wed, 10 Jul 2019 05:46:56 GMT

←Older revision		Revision as of 05:46, 10 July 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6n86 ~~is ON HOLD until Paper Publication~~	+	==Resistance to inhibitors of cholinesterase 8A (Ric8A) protein==
-		+	<StructureSection load='6n86' size='340' side='right'caption='[[6n86]], [[Resolution\|resolution]] 3.90Å' scene=''>
-	~~Authors~~: ~~Srivastava~~, D., ~~Gakhar~~, L., ~~Artemyev~~, N.O.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6n86]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N86 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N86 FirstGlance]. <br>
-	~~Description~~: ~~Resistance~~ to ~~inhibitors~~ of ~~cholinesterase 8A~~ (~~Ric8A~~) protein	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n86 OCA], [http://pdbe.org/6n86 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n86 RCSB], [http://www.ebi.ac.uk/pdbsum/6n86 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n86 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Artemyev, N.O]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RIC8A_BOVIN RIC8A_BOVIN]] Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation (By similarity).
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Artemyev, N O]]
	[[Category: Gakhar, L]]		[[Category: Gakhar, L]]
	[[Category: Srivastava, D]]		[[Category: Srivastava, D]]
		+	[[Category: Armadillo repeat]]
		+	[[Category: Chaperone]]
		+	[[Category: G alpha]]
		+	[[Category: Ric8a]]

OCA at 05:43, 21 May 2019

OCA — Tue, 21 May 2019 05:43:02 GMT

←Older revision		Revision as of 05:43, 21 May 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6n86 is ON HOLD	+	The entry 6n86 is ON HOLD until Paper Publication

	Authors: Srivastava, D., Gakhar, L., Artemyev, N.O.		Authors: Srivastava, D., Gakhar, L., Artemyev, N.O.

OCA at 04:51, 24 April 2019

OCA — Wed, 24 Apr 2019 04:51:03 GMT

←Older revision		Revision as of 04:51, 24 April 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6n86 is ON HOLD ~~until Paper Publication~~	+	The entry 6n86 is ON HOLD

	Authors: Srivastava, D., Gakhar, L., Artemyev, N.O.		Authors: Srivastava, D., Gakhar, L., Artemyev, N.O.

OCA at 04:53, 2 January 2019

OCA — Wed, 02 Jan 2019 04:53:43 GMT

←Older revision		Revision as of 04:53, 2 January 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6n86 is ON HOLD	+	The entry 6n86 is ON HOLD until Paper Publication

	Authors: Srivastava, D., Gakhar, L., Artemyev, N.O.		Authors: Srivastava, D., Gakhar, L., Artemyev, N.O.

-	Description: ~~Apo structure~~ of ~~Ric8a~~	+	Description: Resistance to inhibitors of cholinesterase 8A (Ric8A) protein
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Artemyev, N.O]]		[[Category: Artemyev, N.O]]
	[[Category: Gakhar, L]]		[[Category: Gakhar, L]]
	[[Category: Srivastava, D]]		[[Category: Srivastava, D]]

OCA at 05:20, 12 December 2018

OCA — Wed, 12 Dec 2018 05:20:58 GMT

←Older revision		Revision as of 05:20, 12 December 2018
Line 5:		Line 5:
	Authors: Srivastava, D., Gakhar, L., Artemyev, N.O.		Authors: Srivastava, D., Gakhar, L., Artemyev, N.O.

-	Description: structure of Ric8a ~~in complex with MBP-G alpha t peptide~~	+	Description: Apo structure of Ric8a
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Artemyev, N.O]]		[[Category: Artemyev, N.O]]
	[[Category: Gakhar, L]]		[[Category: Gakhar, L]]
	[[Category: Srivastava, D]]		[[Category: Srivastava, D]]

OCA: Protected "6n86" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2018 05:20:59 GMT

Protected "6n86" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:20, 5 December 2018

OCA: New page: '''Unreleased structure''' The entry 6n86 is ON HOLD Authors: Srivastava, D., Gakhar, L., Artemyev, N.O. Description: structure of Ric8a in complex with MBP-G alpha t peptide [[Categor...

OCA — Wed, 05 Dec 2018 05:20:58 GMT

New page: '''Unreleased structure''' The entry 6n86 is ON HOLD Authors: Srivastava, D., Gakhar, L., Artemyev, N.O. Description: structure of Ric8a in complex with MBP-G alpha t peptide [[Categor...

New page

'''Unreleased structure'''

The entry 6n86 is ON HOLD

Authors: Srivastava, D., Gakhar, L., Artemyev, N.O.

Description: structure of Ric8a in complex with MBP-G alpha t peptide
[[Category: Unreleased Structures]]
[[Category: Artemyev, N.O]]
[[Category: Gakhar, L]]
[[Category: Srivastava, D]]