6n9y - Revision history

OCA at 09:20, 20 March 2024

OCA — Wed, 20 Mar 2024 09:20:54 GMT

←Older revision		Revision as of 09:20, 20 March 2024
Line 3:		Line 3:
	<SX load='6n9y' size='340' side='right' viewer='molstar' caption='[[6n9y]], [[Resolution\|resolution]] 4.00Å' scene=''>		<SX load='6n9y' size='340' side='right' viewer='molstar' caption='[[6n9y]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n9y]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bluetongue_virus_23 Bluetongue virus 23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N9Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6N9Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n9y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bluetongue_virus_23 Bluetongue virus 23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N9Y FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6n9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n9y OCA], [~~http~~://pdbe.org/6n9y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6n9y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6n9y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6n9y ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n9y OCA], [https://pdbe.org/6n9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n9y RCSB], [https://www.ebi.ac.uk/pdbsum/6n9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n9y ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/L7WU69_9VIRU L7WU69_9VIRU]
-	Bluetongue virus (BTV) non-structural protein 1 (NS1) regulates viral protein synthesis and exists as tubular and non-tubular forms in infected cells, but how tubules assemble and how protein synthesis is regulated are unknown. Here, we report near-atomic resolution structures of two NS1 tubular forms determined by cryo-electron microscopy. The two tubular forms are different helical assemblies of the same NS1 monomer, consisting of an amino-terminal foot, a head and body domains connected to an extended carboxy-terminal arm, which wraps atop the head domain of another NS1 subunit through hydrophobic interactions. Deletion of the C terminus prevents tubule formation but not viral replication, suggesting an active non-tubular form. Two zinc-finger-like motifs are present in each NS1 monomer, and tubules are disrupted by divalent cation chelation and restored by cation addition, including Zn(2+), suggesting a regulatory role of divalent cations in tubule formation. In vitro luciferase assays show that the NS1 non-tubular form upregulates BTV mRNA translation, whereas zinc-finger disruption decreases viral mRNA translation, tubule formation and virus replication, confirming a functional role for the zinc-fingers. Thus, the non-tubular form of NS1 is sufficient for viral protein synthesis and infectious virus replication, and the regulatory mechanism involved operates through divalent cation-dependent conversion between the non-tubular and tubular forms.	+

-	Atomic structure of the translation regulatory protein NS1 of bluetongue virus.,Kerviel A, Ge P, Lai M, Jih J, Boyce M, Zhang X, Zhou ZH, Roy P Nat Microbiol. 2019 Feb 18. pii: 10.1038/s41564-019-0369-x. doi:, 10.1038/s41564-019-0369-x. PMID:30778144<ref>PMID:30778144</ref>	+	==See Also==
-		+	*[[Nonstructural protein 3D structures\|Nonstructural protein 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 6n9y" style~~=~~"background-color:#fffaf0;"></div>~~	+
-	~~== References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: Bluetongue virus 23]]		[[Category: Bluetongue virus 23]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boyce, M]]	+	[[Category: Boyce M]]
-	[[Category: Ge, P]]	+	[[Category: Ge P]]
-	[[Category: Jih, J]]	+	[[Category: Jih J]]
-	[[Category: Kerviel, A]]	+	[[Category: Kerviel A]]
-	[[Category: Lai, M]]	+	[[Category: Lai M]]
-	[[Category: Roy, P]]	+	[[Category: Roy P]]
-	[[Category: Zhang, X]]	+	[[Category: Zhang X]]
-	[[Category: Zhou~~, Z H]]~~	+	[[Category: Zhou ZH]]
-	~~[[Category: Bluetongue virus non-structural protein 1]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 03:15, 11 April 2020

OCA — Sat, 11 Apr 2020 03:15:03 GMT

←Older revision		Revision as of 03:15, 11 April 2020
Line 3:		Line 3:
	<SX load='6n9y' size='340' side='right' viewer='molstar' caption='[[6n9y]], [[Resolution\|resolution]] 4.00Å' scene=''>		<SX load='6n9y' size='340' side='right' viewer='molstar' caption='[[6n9y]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6n9y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bluetongue_virus_23 Bluetongue virus 23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N9Y OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6N9Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6n9y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bluetongue_virus_23 Bluetongue virus 23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N9Y OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6N9Y FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6n9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n9y OCA], [http://pdbe.org/6n9y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n9y RCSB], [http://www.ebi.ac.uk/pdbsum/6n9y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n9y ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6n9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n9y OCA], [http://pdbe.org/6n9y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n9y RCSB], [http://www.ebi.ac.uk/pdbsum/6n9y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n9y ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:48, 6 March 2020

OCA — Fri, 06 Mar 2020 22:48:15 GMT

←Older revision		Revision as of 22:48, 6 March 2020
Line 1:		Line 1:

	==Atomic structure of Non-Structural protein 1 of bluetongue virus==		==Atomic structure of Non-Structural protein 1 of bluetongue virus==
-	<~~StructureSection~~ load='6n9y' size='340' side='right'caption='[[6n9y]], [[Resolution\|resolution]] 4.00Å' scene=''>	+	<SX load='6n9y' size='340' side='right' viewer='molstar' caption='[[6n9y]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6n9y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bluetongue_virus_23 Bluetongue virus 23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N9Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N9Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[6n9y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bluetongue_virus_23 Bluetongue virus 23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N9Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N9Y FirstGlance]. <br>
Line 18:		Line 18:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Bluetongue virus 23]]		[[Category: Bluetongue virus 23]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 12:16, 13 March 2019

OCA — Wed, 13 Mar 2019 12:16:01 GMT

←Older revision		Revision as of 12:16, 13 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6n9y is ~~ON HOLD until Paper~~ Publication	+	==Atomic structure of Non-Structural protein 1 of bluetongue virus==
		+	<StructureSection load='6n9y' size='340' side='right'caption='[[6n9y]], [[Resolution\|resolution]] 4.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6n9y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bluetongue_virus_23 Bluetongue virus 23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N9Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N9Y FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n9y OCA], [http://pdbe.org/6n9y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n9y RCSB], [http://www.ebi.ac.uk/pdbsum/6n9y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n9y ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bluetongue virus (BTV) non-structural protein 1 (NS1) regulates viral protein synthesis and exists as tubular and non-tubular forms in infected cells, but how tubules assemble and how protein synthesis is regulated are unknown. Here, we report near-atomic resolution structures of two NS1 tubular forms determined by cryo-electron microscopy. The two tubular forms are different helical assemblies of the same NS1 monomer, consisting of an amino-terminal foot, a head and body domains connected to an extended carboxy-terminal arm, which wraps atop the head domain of another NS1 subunit through hydrophobic interactions. Deletion of the C terminus prevents tubule formation but not viral replication, suggesting an active non-tubular form. Two zinc-finger-like motifs are present in each NS1 monomer, and tubules are disrupted by divalent cation chelation and restored by cation addition, including Zn(2+), suggesting a regulatory role of divalent cations in tubule formation. In vitro luciferase assays show that the NS1 non-tubular form upregulates BTV mRNA translation, whereas zinc-finger disruption decreases viral mRNA translation, tubule formation and virus replication, confirming a functional role for the zinc-fingers. Thus, the non-tubular form of NS1 is sufficient for viral protein synthesis and infectious virus replication, and the regulatory mechanism involved operates through divalent cation-dependent conversion between the non-tubular and tubular forms.

-	~~Authors~~:	+	Atomic structure of the translation regulatory protein NS1 of bluetongue virus.,Kerviel A, Ge P, Lai M, Jih J, Boyce M, Zhang X, Zhou ZH, Roy P Nat Microbiol. 2019 Feb 18. pii: 10.1038/s41564-019-0369-x. doi:, 10.1038/s41564-019-0369-x. PMID:30778144<ref>PMID:30778144</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6n9y" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bluetongue virus 23]]
		+	[[Category: Large Structures]]
		+	[[Category: Boyce, M]]
		+	[[Category: Ge, P]]
		+	[[Category: Jih, J]]
		+	[[Category: Kerviel, A]]
		+	[[Category: Lai, M]]
		+	[[Category: Roy, P]]
		+	[[Category: Zhang, X]]
		+	[[Category: Zhou, Z H]]
		+	[[Category: Bluetongue virus non-structural protein 1]]
		+	[[Category: Viral protein]]

OCA at 10:34, 16 January 2019

OCA — Wed, 16 Jan 2019 10:34:09 GMT

←Older revision		Revision as of 10:34, 16 January 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6n9y is ON HOLD	+	The entry 6n9y is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6n9y" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2018 05:23:55 GMT

Protected "6n9y" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:23, 12 December 2018

OCA: New page: '''Unreleased structure''' The entry 6n9y is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 12 Dec 2018 05:23:54 GMT

New page: '''Unreleased structure''' The entry 6n9y is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6n9y is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]