6nhj - Revision history

OCA at 03:22, 11 April 2020

OCA — Sat, 11 Apr 2020 03:22:22 GMT

←Older revision		Revision as of 03:22, 11 April 2020
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	<SX load='6nhj' size='340' side='right' viewer='molstar' caption='[[6nhj]], [[Resolution\|resolution]] 5.00Å' scene=''>		<SX load='6nhj' size='340' side='right' viewer='molstar' caption='[[6nhj]], [[Resolution\|resolution]] 5.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nhj]] is a 55 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_cytomegalovirus_(strain_smith) Murine cytomegalovirus (strain smith)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHJ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6NHJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nhj]] is a 55 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_cytomegalovirus_(strain_smith) Murine cytomegalovirus (strain smith)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NHJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6nhj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhj OCA], [http://pdbe.org/6nhj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nhj RCSB], [http://www.ebi.ac.uk/pdbsum/6nhj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhj ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nhj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhj OCA], [http://pdbe.org/6nhj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nhj RCSB], [http://www.ebi.ac.uk/pdbsum/6nhj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 04:38, 5 March 2020

OCA — Thu, 05 Mar 2020 04:38:14 GMT

←Older revision		Revision as of 04:38, 5 March 2020
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	==Atomic structures and deletion mutant reveal different capsid-binding patterns and functional significance of tegument protein pp150 in murine and human cytomegaloviruses with implications for therapeutic development==		==Atomic structures and deletion mutant reveal different capsid-binding patterns and functional significance of tegument protein pp150 in murine and human cytomegaloviruses with implications for therapeutic development==
-	<~~StructureSection~~ load='6nhj' size='340' side='right'caption='[[6nhj]], [[Resolution\|resolution]] 5.00Å' scene=''>	+	<SX load='6nhj' size='340' side='right' viewer='molstar' caption='[[6nhj]], [[Resolution\|resolution]] 5.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6nhj]] is a 55 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_cytomegalovirus_(strain_smith) Murine cytomegalovirus (strain smith)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NHJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[6nhj]] is a 55 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_cytomegalovirus_(strain_smith) Murine cytomegalovirus (strain smith)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NHJ FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Balogun, R]]		[[Category: Balogun, R]]

OCA at 08:57, 27 November 2019

OCA — Wed, 27 Nov 2019 08:57:06 GMT

←Older revision		Revision as of 08:57, 27 November 2019
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	==Atomic structures and deletion mutant reveal different capsid-binding patterns and functional significance of tegument protein pp150 in murine and human cytomegaloviruses with implications for therapeutic development==		==Atomic structures and deletion mutant reveal different capsid-binding patterns and functional significance of tegument protein pp150 in murine and human cytomegaloviruses with implications for therapeutic development==
-	<StructureSection load='6nhj' size='340' side='right' caption='[[6nhj]], [[Resolution\|resolution]] 5.00Å' scene=''>	+	<StructureSection load='6nhj' size='340' side='right'caption='[[6nhj]], [[Resolution\|resolution]] 5.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6nhj]] is a 55 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_cytomegalovirus_(strain_smith) Murine cytomegalovirus (strain smith)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NHJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[6nhj]] is a 55 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_cytomegalovirus_(strain_smith) Murine cytomegalovirus (strain smith)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NHJ FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Balogun, R]]		[[Category: Balogun, R]]
	[[Category: Chan, K]]		[[Category: Chan, K]]

OCA at 07:35, 6 March 2019

OCA — Wed, 06 Mar 2019 07:35:45 GMT

←Older revision		Revision as of 07:35, 6 March 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6nhj is ~~ON HOLD~~	+	==Atomic structures and deletion mutant reveal different capsid-binding patterns and functional significance of tegument protein pp150 in murine and human cytomegaloviruses with implications for therapeutic development==
		+	<StructureSection load='6nhj' size='340' side='right' caption='[[6nhj]], [[Resolution\|resolution]] 5.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6nhj]] is a 55 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_cytomegalovirus_(strain_smith) Murine cytomegalovirus (strain smith)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NHJ FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nhj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhj OCA], [http://pdbe.org/6nhj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nhj RCSB], [http://www.ebi.ac.uk/pdbsum/6nhj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhj ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A0A1S5YGR4_MUHV1 A0A1S5YGR4_MUHV1]] Self-assembles to form an icosahedral capsid with a T=16 symmetry, about 200 nm in diameter, and consisting of 150 hexons and 12 pentons (total of 162 capsomers). Hexons form the edges and faces of the capsid and are each composed of six MCP molecules. In contrast, one penton is found at each of the 12 vertices. Eleven of the pentons are MCP pentamers, while the last vertex is occupied by the portal complex. The capsid is surrounded by a layer of proteinaceous material designated the tegument which, in turn, is enclosed in an envelope of host cell-derived lipids containing virus-encoded glycoproteins.[HAMAP-Rule:MF_04016] [[http://www.uniprot.org/uniprot/D3XDN6_MUHVS D3XDN6_MUHVS]] Participates in the assembly of the infectious particles by decorating the outer surface of the capsid shell and thus forming a layer between the capsid and the tegument. Complexes composed of the major capsid protein and small capsomere-interacting protein/SCP assemble together in the host cytoplasm and are translocated to the nucleus, where they accumulate and participate in capsid assembly.[HAMAP-Rule:MF_04021] [[http://www.uniprot.org/uniprot/D3XDR2_MUHVS D3XDR2_MUHVS]] Structural component of the T=16 icosahedral capsid. The capsid is composed of pentamers and hexamers of major capsid protein/MCP, which are linked together by heterotrimers called triplexes. These triplexes are formed by a single molecule of triplex protein 1/TRX1 and two copies of triplex protein 2/TRX2. Additionally, TRX1 is required for efficient transport of TRX2 to the nucleus, which is the site of capsid assembly.[HAMAP-Rule:MF_04019]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytomegalovirus (CMV) infection causes birth defects and life-threatening complications in immunosuppressed patients. Lack of vaccine and need for more effective drugs have driven widespread ongoing therapeutic development efforts against human CMV (HCMV), mostly using murine CMV (MCMV) as the model system for preclinical animal tests. The recent publication (Yu et al., 2017, DOI: 10.1126/science.aam6892) of an atomic model for HCMV capsid with associated tegument protein pp150 has infused impetus for rational design of novel vaccines and drugs, but the absence of high-resolution structural data on MCMV remains a significant knowledge gap in such development efforts. Here, by cryoEM with sub-particle reconstruction method, we have obtained the first atomic structure of MCMV capsid with associated pp150. Surprisingly, the capsid-binding patterns of pp150 differ between HCMV and MCMV despite their highly similar capsid structures. In MCMV, pp150 is absent on triplex Tc and exists as a "Lambda"-shaped dimer on other triplexes, leading to only 260 groups of two pp150 subunits per capsid in contrast to 320 groups of three pp150 subunits each in a "Delta"-shaped fortifying configuration. Many more amino acids contribute to pp150-pp150 interactions in MCMV than in HCMV, making MCMV pp150 dimer inflexible thus incompatible to instigate triplex Tc-binding as observed in HCMV. While pp150 is essential in HCMV, our pp150-deletion mutant of MCMV remained viable though with attenuated infectivity and exhibiting defects in retaining viral genome. These results thus invalidate targeting pp150, but lend support to targeting capsid proteins, when using MCMV as a model for HCMV pathogenesis and therapeutic studies.

-	~~Authors~~:	+	Atomic structures and deletion mutant reveal different capsid-binding patterns and functional significance of tegument protein pp150 in murine and human cytomegaloviruses with implications for therapeutic development.,Liu W, Dai X, Jih J, Chan K, Trang P, Yu X, Balogun R, Mei Y, Liu F, Zhou ZH PLoS Pathog. 2019 Feb 19;15(2):e1007615. doi: 10.1371/journal.ppat.1007615., eCollection 2019 Feb. PMID:30779794<ref>PMID:30779794</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6nhj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Balogun, R]]
		+	[[Category: Chan, K]]
		+	[[Category: Dai, X H]]
		+	[[Category: Jih, J]]
		+	[[Category: Liu, F Y]]
		+	[[Category: Liu, W]]
		+	[[Category: Mei, Y]]
		+	[[Category: Trang, P]]
		+	[[Category: Yu, X K]]
		+	[[Category: Zhou, Z H]]
		+	[[Category: Bacterial artificial chromosome-based mutagenesis]]
		+	[[Category: Beta-herpesvirus]]
		+	[[Category: Cryoem]]
		+	[[Category: Human cytomegalovirus]]
		+	[[Category: Murine cytomegalovirus]]
		+	[[Category: Pm32]]
		+	[[Category: Pp150]]
		+	[[Category: Pul32]]
		+	[[Category: Virus]]

OCA: Protected "6nhj" [edit=sysop:move=sysop]

OCA — Wed, 02 Jan 2019 04:57:27 GMT

Protected "6nhj" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:57, 2 January 2019

OCA: New page: '''Unreleased structure''' The entry 6nhj is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Jan 2019 04:57:26 GMT

New page: '''Unreleased structure''' The entry 6nhj is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6nhj is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]