6nhr - Revision history

OCA at 06:54, 11 October 2023

OCA — Wed, 11 Oct 2023 06:54:03 GMT

←Older revision		Revision as of 06:54, 11 October 2023
Line 3:		Line 3:
	<StructureSection load='6nhr' size='340' side='right'caption='[[6nhr]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6nhr' size='340' side='right'caption='[[6nhr]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nhr]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~I68a4 I68a4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6NHR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nhr]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Hong_Kong/1/1968(H3N2)) Influenza A virus (A/Hong Kong/1/1968(H3N2))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NHR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BMA:BETA~~-~~D-MANNOSE'>BMA</scene>~~, ~~<scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~UNK~~:~~UNKNOWN~~'>~~UNK~~</scene><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">HA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=506350 I68A4])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhr OCA], [https://pdbe.org/6nhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nhr RCSB], [https://www.ebi.ac.uk/pdbsum/6nhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhr ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6nhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhr OCA], [~~http~~://pdbe.org/6nhr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6nhr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6nhr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhr ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/H9XC94_I68A4 H9XC94_I68A4]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[HAMAP-Rule:MF_04072][SAAS:SAAS01039073] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324] ~~[[http~~:~~//www~~.~~uniprot~~.~~org/uniprot/HEMA_I68A4 HEMA_I68A4]] Binds to sialic acid-containing receptors~~ on ~~the cell surface~~, ~~bringing about~~ the ~~attachment of~~ the ~~virus particle~~ to the ~~cell~~. ~~This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis~~ and ~~about one third through a clathrin-~~ and ~~caveolin-independent pathway~~. ~~Plays~~ a ~~major role in the determination~~ of ~~host range restriction and virulence~~. ~~Class I viral fusion protein. Responsible~~ for ~~penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane~~. ~~Low pH in endosomes induces an irreversible conformational change in HA2~~, ~~releasing the fusion hydrophobic peptide~~. ~~Several trimers are required to form a competent fusion pore~~ (~~By similarity~~).	+	[https://www.uniprot.org/uniprot/H9XC94_I68A4 H9XC94_I68A4] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[HAMAP-Rule:MF_04072][SAAS:SAAS01039073] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The discovery and characterization of broadly neutralizing human antibodies (bnAbs) to the highly conserved stem region of influenza hemagglutinin (HA) have contributed to considerations of a universal influenza vaccine. However, the potential for resistance to stem bnAbs also needs to be more thoroughly evaluated. Using deep mutational scanning, with a focus on epitope residues, we found that the genetic barrier to resistance to stem bnAbs is low for the H3 subtype but substantially higher for the H1 subtype owing to structural differences in the HA stem. Several strong resistance mutations in H3 can be observed in naturally circulating strains and do not reduce in vitro viral fitness and in vivo pathogenicity. This study highlights a potential challenge for development of a truly universal influenza vaccine.
		+
		+	Different genetic barriers for resistance to HA stem antibodies in influenza H3 and H1 viruses.,Wu NC, Thompson AJ, Lee JM, Su W, Arlian BM, Xie J, Lerner RA, Yen HL, Bloom JD, Wilson IA Science. 2020 Jun 19;368(6497):1335-1340. doi: 10.1126/science.aaz5143. PMID:32554590<ref>PMID:32554590</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6nhr" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Hemagglutinin 3D structures\|Hemagglutinin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: I68a4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Wilson~~, I A~~]]	+	[[Category: Wilson IA]]
-	[[Category: Wu~~, N C]]~~	+	[[Category: Wu NC]]
-	~~[[Category: Viral protein~~]]	+

OCA at 10:35, 17 June 2020

OCA — Wed, 17 Jun 2020 10:35:17 GMT

←Older revision		Revision as of 10:35, 17 June 2020
Line 1:		Line 1:

	==Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin HA2 I45F mutant==		==Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin HA2 I45F mutant==
-	<StructureSection load='6nhr' size='340' side='right'caption='[[6nhr]]' scene=''>	+	<StructureSection load='6nhr' size='340' side='right'caption='[[6nhr]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NHR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nhr]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/I68a4 I68a4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NHR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhr OCA], [http://pdbe.org/6nhr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nhr RCSB], [http://www.ebi.ac.uk/pdbsum/6nhr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhr ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=506350 I68A4])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhr OCA], [http://pdbe.org/6nhr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nhr RCSB], [http://www.ebi.ac.uk/pdbsum/6nhr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/H9XC94_I68A4 H9XC94_I68A4]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[HAMAP-Rule:MF_04072][SAAS:SAAS01039073] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324] [[http://www.uniprot.org/uniprot/HEMA_I68A4 HEMA_I68A4]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: I68a4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Wilson IA]]	+	[[Category: Wilson, I A]]
-	[[Category: Wu NC]]	+	[[Category: Wu, N C]]
		+	[[Category: Viral protein]]

OCA at 06:54, 15 April 2020

OCA — Wed, 15 Apr 2020 06:54:14 GMT

←Older revision		Revision as of 06:54, 15 April 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6nhr is ON HOLD until Paper Publication~~	+	==Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin HA2 I45F mutant==
-		+	<StructureSection load='6nhr' size='340' side='right'caption='[[6nhr]]' scene=''>
-	~~Authors: Wu, N.C., Wilson, I.A.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NHR FirstGlance]. <br>
-	~~Description:~~ Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin HA2 I45F mutant	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhr OCA], [http://pdbe.org/6nhr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nhr RCSB], [http://www.ebi.ac.uk/pdbsum/6nhr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhr ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: Wu, N.C]]	+	__TOC__
-	[[Category: Wilson~~, I.A~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Wilson IA]]
		+	[[Category: Wu NC]]

OCA at 05:04, 21 February 2019

OCA — Thu, 21 Feb 2019 05:04:26 GMT

←Older revision		Revision as of 05:04, 21 February 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6nhr is ON HOLD	+	The entry 6nhr is ON HOLD until Paper Publication

	Authors: Wu, N.C., Wilson, I.A.		Authors: Wu, N.C., Wilson, I.A.

OCA: Protected "6nhr" [edit=sysop:move=sysop]

OCA — Wed, 02 Jan 2019 04:58:24 GMT

Protected "6nhr" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:58, 2 January 2019

OCA: New page: '''Unreleased structure''' The entry 6nhr is ON HOLD Authors: Wu, N.C., Wilson, I.A. Description: Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin HA2 I...

OCA — Wed, 02 Jan 2019 04:58:23 GMT

New page: '''Unreleased structure''' The entry 6nhr is ON HOLD Authors: Wu, N.C., Wilson, I.A. Description: Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin HA2 I...

New page

'''Unreleased structure'''

The entry 6nhr is ON HOLD

Authors: Wu, N.C., Wilson, I.A.

Description: Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin HA2 I45F mutant
[[Category: Unreleased Structures]]
[[Category: Wu, N.C]]
[[Category: Wilson, I.A]]