6nhv - Revision history

OCA at 03:22, 11 April 2020

OCA — Sat, 11 Apr 2020 03:22:49 GMT

←Older revision		Revision as of 03:22, 11 April 2020
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	<SX load='6nhv' size='340' side='right' viewer='molstar' caption='[[6nhv]], [[Resolution\|resolution]] 3.50Å' scene=''>		<SX load='6nhv' size='340' side='right' viewer='molstar' caption='[[6nhv]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nhv]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi], [http://en.wikipedia.org/wiki/Pseae Pseae] and [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHV OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6NHV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nhv]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi], [http://en.wikipedia.org/wiki/Pseae Pseae] and [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NHV FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA1966 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA1966 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6nhv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhv OCA], [http://pdbe.org/6nhv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nhv RCSB], [http://www.ebi.ac.uk/pdbsum/6nhv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nhv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhv OCA], [http://pdbe.org/6nhv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nhv RCSB], [http://www.ebi.ac.uk/pdbsum/6nhv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:51, 6 March 2020

OCA — Fri, 06 Mar 2020 22:51:21 GMT

←Older revision		Revision as of 22:51, 6 March 2020
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	==Single particle reconstruction of DARPin and its bound GFP on a symmetric scaffold==		==Single particle reconstruction of DARPin and its bound GFP on a symmetric scaffold==
-	<~~StructureSection~~ load='6nhv' size='340' side='right'caption='[[6nhv]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<SX load='6nhv' size='340' side='right' viewer='molstar' caption='[[6nhv]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6nhv]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi], [http://en.wikipedia.org/wiki/Pseae Pseae] and [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NHV FirstGlance]. <br>		<table><tr><td colspan='2'>[[6nhv]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi], [http://en.wikipedia.org/wiki/Pseae Pseae] and [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NHV FirstGlance]. <br>
Line 20:		Line 20:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Aeqvi]]		[[Category: Aeqvi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 08:57, 27 November 2019

OCA — Wed, 27 Nov 2019 08:57:46 GMT

←Older revision		Revision as of 08:57, 27 November 2019
Line 3:		Line 3:
	<StructureSection load='6nhv' size='340' side='right'caption='[[6nhv]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='6nhv' size='340' side='right'caption='[[6nhv]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nhv]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NHV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nhv]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi], [http://en.wikipedia.org/wiki/Pseae Pseae] and [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NHV FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA1966 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nhv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhv OCA], [http://pdbe.org/6nhv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nhv RCSB], [http://www.ebi.ac.uk/pdbsum/6nhv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nhv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhv OCA], [http://pdbe.org/6nhv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nhv RCSB], [http://www.ebi.ac.uk/pdbsum/6nhv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhv ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aeqvi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Pseae]]
		+	[[Category: Pyrococcus horikoshii]]
	[[Category: Huynh, D]]		[[Category: Huynh, D]]
	[[Category: Liu, Y]]		[[Category: Liu, Y]]

OCA at 11:21, 10 May 2019

OCA — Fri, 10 May 2019 11:21:22 GMT

←Older revision		Revision as of 11:21, 10 May 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6nhv is ~~ON HOLD until Paper~~ Publication	+	==Single particle reconstruction of DARPin and its bound GFP on a symmetric scaffold==
		+	<StructureSection load='6nhv' size='340' side='right'caption='[[6nhv]], [[Resolution\|resolution]] 3.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6nhv]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NHV FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nhv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhv OCA], [http://pdbe.org/6nhv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nhv RCSB], [http://www.ebi.ac.uk/pdbsum/6nhv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhv ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proteins smaller than about 50 kDa are currently too small to be imaged at high resolution by cryo-electron microscopy (cryo-EM), leaving most protein molecules in the cell beyond the reach of this powerful structural technique. Here we use a designed protein scaffold to bind and symmetrically display 12 copies of a small 26 kDa protein, green fluorescent protein (GFP). We show that the bound cargo protein is held rigidly enough to visualize it at a resolution of 3.8 A by cryo-EM, where specific structural features of the protein are visible. The designed scaffold is modular and can be modified through modest changes in its amino acid sequence to bind and display diverse proteins for imaging, thus providing a general method to break through the lower size limitation in cryo-EM.

-	~~Authors~~:	+	A 3.8 A resolution cryo-EM structure of a small protein bound to an imaging scaffold.,Liu Y, Huynh DT, Yeates TO Nat Commun. 2019 Apr 23;10(1):1864. doi: 10.1038/s41467-019-09836-0. PMID:31015551<ref>PMID:31015551</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6nhv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Huynh, D]]
		+	[[Category: Liu, Y]]
		+	[[Category: Yeates, T O]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Display platform]]
		+	[[Category: Protein engineering]]
		+	[[Category: Small protein cryo-em]]
		+	[[Category: Symmetric scaffold]]

OCA at 06:31, 30 January 2019

OCA — Wed, 30 Jan 2019 06:31:21 GMT

←Older revision		Revision as of 06:31, 30 January 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6nhv is ON HOLD	+	The entry 6nhv is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6nhv" [edit=sysop:move=sysop]

OCA — Wed, 02 Jan 2019 04:58:40 GMT

Protected "6nhv" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:58, 2 January 2019

OCA: New page: '''Unreleased structure''' The entry 6nhv is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Jan 2019 04:58:38 GMT

New page: '''Unreleased structure''' The entry 6nhv is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6nhv is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]