6nj8 - Revision history

OCA at 09:21, 20 March 2024

OCA — Wed, 20 Mar 2024 09:21:19 GMT

←Older revision		Revision as of 09:21, 20 March 2024
Line 3:		Line 3:
	<SX load='6nj8' size='340' side='right' viewer='molstar' caption='[[6nj8]], [[Resolution\|resolution]] 3.85Å' scene=''>		<SX load='6nj8' size='340' side='right' viewer='molstar' caption='[[6nj8]], [[Resolution\|resolution]] 3.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nj8]] is a 7 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"quasibacillus_thermotolerans"_(yang_et_al._2013)_verma_et_al._2017 "quasibacillus~~ thermotolerans~~" (yang et al. 2013) verma et al. 2017~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJ8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6NJ8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nj8]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Quasibacillus_thermotolerans Quasibacillus thermotolerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NJ8 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[6n63\|6n63]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.85Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">QY95_01592 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=1221996 "Quasibacillus thermotolerans" (Yang et al. 2013) Verma et al. 2017])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nj8 OCA], [https://pdbe.org/6nj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nj8 RCSB], [https://www.ebi.ac.uk/pdbsum/6nj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nj8 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6nj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nj8 OCA], [~~http~~://pdbe.org/6nj8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6nj8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6nj8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6nj8 ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/ENCAP_QUATH ENCAP_QUATH]
-	Iron storage proteins are essential for cellular iron homeostasis and redox balance. Ferritin proteins are the major storage units for bioavailable forms of iron. Some organisms lack ferritins, and it is not known how they store iron. Encapsulins, a class of protein-based organelles, have recently been implicated in microbial iron and redox metabolism. Here, we report the structural and mechanistic characterization of a 42 nm two-component encapsulin-based iron storage compartment from Quasibacillus thermotolerans. Using cryo-electron microscopy and x-ray crystallography, we reveal the assembly principles of a thermostable T = 4 shell topology and its catalytic ferroxidase cargo and show interactions underlying cargo-shell co-assembly. This compartment has an exceptionally large iron storage capacity storing over 23,000 iron atoms. Our results reveal a new approach for survival in diverse habitats with limited or fluctuating iron availability via an iron storage system able to store 10 to 20 times more iron than ferritin.	+
-		+
-	Large protein organelles form a new iron sequestration system with high storage capacity.,Giessen TW, Orlando BJ, Verdegaal AA, Chambers MG, Gardener J, Bell DC, Birrane G, Liao M, Silver PA Elife. 2019 Jul 8;8. pii: ~~46070. doi: 10.7554~~/~~eLife.46070. PMID:31282860<ref>PMID:31282860<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6nj8" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chambers, M G~~]]	+	[[Category: Quasibacillus thermotolerans]]
-	[[Category: ~~Giessen, T W~~]]	+	[[Category: Chambers MG]]
-	[[Category: ~~Liao, M~~]]	+	[[Category: Giessen TW]]
-	[[Category: ~~Orlando, B J~~]]	+	[[Category: Liao M]]
-	[[Category: ~~Silver, P A~~]]	+	[[Category: Orlando BJ]]
-	[[Category: ~~Encapsulin]]~~	+	[[Category: Silver PA]]
-	~~[[Category: Icosahedral]]~~	+
-	~~[[Category: Imef]]~~	+
-	~~[[Category: Iron storage]]~~	+
-	~~[[Category: Metal transport~~]]	+

OCA at 03:24, 11 April 2020

OCA — Sat, 11 Apr 2020 03:24:06 GMT

←Older revision		Revision as of 03:24, 11 April 2020
Line 3:		Line 3:
	<SX load='6nj8' size='340' side='right' viewer='molstar' caption='[[6nj8]], [[Resolution\|resolution]] 3.85Å' scene=''>		<SX load='6nj8' size='340' side='right' viewer='molstar' caption='[[6nj8]], [[Resolution\|resolution]] 3.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nj8]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/"quasibacillus_thermotolerans"_(yang_et_al._2013)_verma_et_al._2017 "quasibacillus thermotolerans" (yang et al. 2013) verma et al. 2017]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6NJ8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nj8]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/"quasibacillus_thermotolerans"_(yang_et_al._2013)_verma_et_al._2017 "quasibacillus thermotolerans" (yang et al. 2013) verma et al. 2017]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NJ8 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6n63\|6n63]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6n63\|6n63]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QY95_01592 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1221996 "Quasibacillus thermotolerans" (Yang et al. 2013) Verma et al. 2017])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QY95_01592 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1221996 "Quasibacillus thermotolerans" (Yang et al. 2013) Verma et al. 2017])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6nj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nj8 OCA], [http://pdbe.org/6nj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nj8 RCSB], [http://www.ebi.ac.uk/pdbsum/6nj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nj8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nj8 OCA], [http://pdbe.org/6nj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nj8 RCSB], [http://www.ebi.ac.uk/pdbsum/6nj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nj8 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:51, 6 March 2020

OCA — Fri, 06 Mar 2020 22:51:50 GMT

←Older revision		Revision as of 22:51, 6 March 2020
Line 1:		Line 1:

	==Encapsulin iron storage compartment from Quasibacillus thermotolerans==		==Encapsulin iron storage compartment from Quasibacillus thermotolerans==
-	<~~StructureSection~~ load='6nj8' size='340' side='right'caption='[[6nj8]], [[Resolution\|resolution]] 3.85Å' scene=''>	+	<SX load='6nj8' size='340' side='right' viewer='molstar' caption='[[6nj8]], [[Resolution\|resolution]] 3.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6nj8]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/"quasibacillus_thermotolerans"_(yang_et_al._2013)_verma_et_al._2017 "quasibacillus thermotolerans" (yang et al. 2013) verma et al. 2017]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NJ8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6nj8]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/"quasibacillus_thermotolerans"_(yang_et_al._2013)_verma_et_al._2017 "quasibacillus thermotolerans" (yang et al. 2013) verma et al. 2017]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NJ8 FirstGlance]. <br>
Line 20:		Line 20:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chambers, M G]]		[[Category: Chambers, M G]]

OCA at 10:35, 4 December 2019

OCA — Wed, 04 Dec 2019 10:35:28 GMT

←Older revision		Revision as of 10:35, 4 December 2019
Line 3:		Line 3:
	<StructureSection load='6nj8' size='340' side='right'caption='[[6nj8]], [[Resolution\|resolution]] 3.85Å' scene=''>		<StructureSection load='6nj8' size='340' side='right'caption='[[6nj8]], [[Resolution\|resolution]] 3.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nj8]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NJ8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nj8]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/"quasibacillus_thermotolerans"_(yang_et_al._2013)_verma_et_al._2017 "quasibacillus thermotolerans" (yang et al. 2013) verma et al. 2017]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NJ8 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6n63\|6n63]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6n63\|6n63]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QY95_01592 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1221996 "Quasibacillus thermotolerans" (Yang et al. 2013) Verma et al. 2017])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nj8 OCA], [http://pdbe.org/6nj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nj8 RCSB], [http://www.ebi.ac.uk/pdbsum/6nj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nj8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nj8 OCA], [http://pdbe.org/6nj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nj8 RCSB], [http://www.ebi.ac.uk/pdbsum/6nj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nj8 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Iron storage proteins are essential for cellular iron homeostasis and redox balance. Ferritin proteins are the major storage units for bioavailable forms of iron. Some organisms lack ferritins, and it is not known how they store iron. Encapsulins, a class of protein-based organelles, have recently been implicated in microbial iron and redox metabolism. Here, we report the structural and mechanistic characterization of a 42 nm two-component encapsulin-based iron storage compartment from Quasibacillus thermotolerans. Using cryo-electron microscopy and x-ray crystallography, we reveal the assembly principles of a thermostable T = 4 shell topology and its catalytic ferroxidase cargo and show interactions underlying cargo-shell co-assembly. This compartment has an exceptionally large iron storage capacity storing over 23,000 iron atoms. Our results reveal a new approach for survival in diverse habitats with limited or fluctuating iron availability via an iron storage system able to store 10 to 20 times more iron than ferritin.
		+
		+	Large protein organelles form a new iron sequestration system with high storage capacity.,Giessen TW, Orlando BJ, Verdegaal AA, Chambers MG, Gardener J, Bell DC, Birrane G, Liao M, Silver PA Elife. 2019 Jul 8;8. pii: 46070. doi: 10.7554/eLife.46070. PMID:31282860<ref>PMID:31282860</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6nj8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 05:55, 7 August 2019

OCA — Wed, 07 Aug 2019 05:55:43 GMT

←Older revision		Revision as of 05:55, 7 August 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6nj8 ~~is ON HOLD until Paper Publication~~	+	==Encapsulin iron storage compartment from Quasibacillus thermotolerans==
-		+	<StructureSection load='6nj8' size='340' side='right'caption='[[6nj8]], [[Resolution\|resolution]] 3.85Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6nj8]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NJ8 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6n63\|6n63]]</td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nj8 OCA], [http://pdbe.org/6nj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nj8 RCSB], [http://www.ebi.ac.uk/pdbsum/6nj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nj8 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chambers, M G]]
		+	[[Category: Giessen, T W]]
		+	[[Category: Liao, M]]
		+	[[Category: Orlando, B J]]
		+	[[Category: Silver, P A]]
		+	[[Category: Encapsulin]]
		+	[[Category: Icosahedral]]
		+	[[Category: Imef]]
		+	[[Category: Iron storage]]
		+	[[Category: Metal transport]]

OCA at 06:32, 30 January 2019

OCA — Wed, 30 Jan 2019 06:32:12 GMT

←Older revision		Revision as of 06:32, 30 January 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6nj8 is ON HOLD	+	The entry 6nj8 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6nj8" [edit=sysop:move=sysop]

OCA — Wed, 09 Jan 2019 05:06:51 GMT

Protected "6nj8" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:06, 9 January 2019

OCA: New page: '''Unreleased structure''' The entry 6nj8 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 09 Jan 2019 05:06:50 GMT

New page: '''Unreleased structure''' The entry 6nj8 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6nj8 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]