6nla - Revision history

OCA at 09:15, 25 October 2023

OCA — Wed, 25 Oct 2023 09:15:41 GMT

←Older revision		Revision as of 09:15, 25 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6nla]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus Streptococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NLA FirstGlance]. <br>		<table><tr><td colspan='2'>[[6nla]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus Streptococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NLA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.34Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nla OCA], [https://pdbe.org/6nla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nla RCSB], [https://www.ebi.ac.uk/pdbsum/6nla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nla ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nla OCA], [https://pdbe.org/6nla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nla RCSB], [https://www.ebi.ac.uk/pdbsum/6nla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nla ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:36, 3 May 2023

OCA — Wed, 03 May 2023 07:36:35 GMT

←Older revision		Revision as of 07:36, 3 May 2023
Line 3:		Line 3:
	<StructureSection load='6nla' size='340' side='right'caption='[[6nla]], [[Resolution\|resolution]] 1.34Å' scene=''>		<StructureSection load='6nla' size='340' side='right'caption='[[6nla]], [[Resolution\|resolution]] 1.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nla]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~9stre 9stre~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NLA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6NLA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nla]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus Streptococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NLA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1301 9STRE])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nla OCA], [https://pdbe.org/6nla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nla RCSB], [https://www.ebi.ac.uk/pdbsum/6nla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nla ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6nla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nla OCA], [~~http~~://pdbe.org/6nla PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6nla RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6nla PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6nla ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPG2_STRSG SPG2_STRSG]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 6nla" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6nla" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Protein G\|Protein G]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Boguslaw, S~~]]	+	[[Category: Streptococcus]]
-	[[Category: Huxford, T]]	+	[[Category: Huxford T]]
-	[[Category: Maniaci, B]]	+	[[Category: Maniaci B]]
-	[[Category: ~~B1 domain of streptococcal protein g]]~~	+	[[Category: Stec B]]
-	~~[[Category: De novo protein]]~~	+
-	~~[[Category: Immunoglobulin binding protein]]~~	+
-	~~[[Category: Metal-mediated dimer~~]]	+

OCA at 08:21, 21 May 2019

OCA — Tue, 21 May 2019 08:21:43 GMT

←Older revision		Revision as of 08:21, 21 May 2019
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Boguslaw, S]]
	[[Category: Huxford, T]]		[[Category: Huxford, T]]
	[[Category: Maniaci, B]]		[[Category: Maniaci, B]]

OCA at 13:42, 10 May 2019

OCA — Fri, 10 May 2019 13:42:39 GMT

←Older revision		Revision as of 13:42, 10 May 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6nla is ~~ON HOLD~~	+	==Crystal structure of de novo designed metal-controlled dimer of B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, E15V, T16L, T18I, V29H, Y33H, N37L)-zinc==
		+	<StructureSection load='6nla' size='340' side='right'caption='[[6nla]], [[Resolution\|resolution]] 1.34Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6nla]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/9stre 9stre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NLA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NLA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1301 9STRE])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nla OCA], [http://pdbe.org/6nla PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nla RCSB], [http://www.ebi.ac.uk/pdbsum/6nla PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nla ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ability to precisely control protein complex formation has high utility in the expanding field of biomaterials. Driving protein-protein binding through metal-ligand bridging interactions is a promising method of achieving this goal. Furthermore, the capacity to precisely regulate both complex formation and dissociation enables additional control not available with constitutive protein complexes. Here we describe the design of three metal-controlled protein dimers that are completely monomeric in the absence of metal yet form high-affinity symmetric homodimers in the presence of zinc sulfate. The scaffold used for the designed dimers is the beta1 domain of streptococcal protein G. In addition to forming high-affinity dimers in the presence of metal, the complexes also dissociate upon addition of EDTA. Biophysical characterization revealed that the proteins maintain relatively high thermal stability, bind with high affinity, and are completely monodisperse in the monomeric and dimeric states. High-resolution crystal structures revealed that the dimers adopt the target structure and that the designed metal-binding histidine residues successfully bind zinc and function to drive dimer formation.

-	~~Authors: Huxford, T~~., Maniaci, B.	+	Design of High-Affinity Metal-Controlled Protein Dimers.,Maniaci B, Lipper CH, Anipindi DL, Erlandsen H, Cole JL, Stec B, Huxford T, Love JJ Biochemistry. 2019 Apr 30;58(17):2199-2207. doi: 10.1021/acs.biochem.9b00055., Epub 2019 Apr 12. PMID:30938154<ref>PMID:30938154</ref>

-	~~Description: Crystal structure~~ of ~~de novo designed metal-controlled dimer~~ of ~~B1 immunoglobulin~~-~~binding domain of Streptococcal Protein G (L12H, E15V, T16L, T18I, V29H, Y33H, N37L)~~-~~zinc~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6nla" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Huxford, T]]		[[Category: Huxford, T]]
	[[Category: Maniaci, B]]		[[Category: Maniaci, B]]
		+	[[Category: B1 domain of streptococcal protein g]]
		+	[[Category: De novo protein]]
		+	[[Category: Immunoglobulin binding protein]]
		+	[[Category: Metal-mediated dimer]]

OCA: Protected "6nla" [edit=sysop:move=sysop]

OCA — Wed, 16 Jan 2019 10:43:45 GMT

Protected "6nla" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:43, 16 January 2019

OCA: New page: '''Unreleased structure''' The entry 6nla is ON HOLD Authors: Huxford, T., Maniaci, B. Description: Crystal structure of de novo designed metal-controlled dimer of B1 immunoglobulin-bi...

OCA — Wed, 16 Jan 2019 10:43:43 GMT

New page: '''Unreleased structure''' The entry 6nla is ON HOLD Authors: Huxford, T., Maniaci, B. Description: Crystal structure of de novo designed metal-controlled dimer of B1 immunoglobulin-bi...

New page

'''Unreleased structure'''

The entry 6nla is ON HOLD

Authors: Huxford, T., Maniaci, B.

Description: Crystal structure of de novo designed metal-controlled dimer of B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, E15V, T16L, T18I, V29H, Y33H, N37L)-zinc
[[Category: Unreleased Structures]]
[[Category: Huxford, T]]
[[Category: Maniaci, B]]