6nq0 - Revision history

OCA at 03:28, 11 April 2020

OCA — Sat, 11 Apr 2020 03:28:44 GMT

←Older revision		Revision as of 03:28, 11 April 2020
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	<SX load='6nq0' size='340' side='right' viewer='molstar' caption='[[6nq0]], [[Resolution\|resolution]] 3.70Å' scene=''>		<SX load='6nq0' size='340' side='right' viewer='molstar' caption='[[6nq0]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nq0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6NQ0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nq0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NQ0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EUJ:(2R)-3-{[(S)-hydroxy{[(1S,2R,3R,4S,5S,6R)-2,4,6-trihydroxy-3,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl+dioctanoate'>EUJ</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EUJ:(2R)-3-{[(S)-hydroxy{[(1S,2R,3R,4S,5S,6R)-2,4,6-trihydroxy-3,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl+dioctanoate'>EUJ</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPCN2, TPC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPCN2, TPC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6nq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nq0 OCA], [http://pdbe.org/6nq0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nq0 RCSB], [http://www.ebi.ac.uk/pdbsum/6nq0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nq0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nq0 OCA], [http://pdbe.org/6nq0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nq0 RCSB], [http://www.ebi.ac.uk/pdbsum/6nq0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nq0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 22:53, 6 March 2020

OCA — Fri, 06 Mar 2020 22:53:38 GMT

←Older revision		Revision as of 22:53, 6 March 2020
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	==Cryo-EM structure of human TPC2 channel in the ligand-bound open state==		==Cryo-EM structure of human TPC2 channel in the ligand-bound open state==
-	<~~StructureSection~~ load='6nq0' size='340' side='right'caption='[[6nq0]], [[Resolution\|resolution]] 3.70Å' scene=''>	+	<SX load='6nq0' size='340' side='right' viewer='molstar' caption='[[6nq0]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6nq0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NQ0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6nq0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NQ0 FirstGlance]. <br>
Line 25:		Line 25:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Human]]		[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 17:36, 20 November 2019

OCA — Wed, 20 Nov 2019 17:36:55 GMT

←Older revision		Revision as of 17:36, 20 November 2019
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	<StructureSection load='6nq0' size='340' side='right'caption='[[6nq0]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='6nq0' size='340' side='right'caption='[[6nq0]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nq0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NQ0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nq0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NQ0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EUJ:(2R)-3-{[(S)-hydroxy{[(1S,2R,3R,4S,5S,6R)-2,4,6-trihydroxy-3,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl+dioctanoate'>EUJ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EUJ:(2R)-3-{[(S)-hydroxy{[(1S,2R,3R,4S,5S,6R)-2,4,6-trihydroxy-3,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl+dioctanoate'>EUJ</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPCN2, TPC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nq0 OCA], [http://pdbe.org/6nq0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nq0 RCSB], [http://www.ebi.ac.uk/pdbsum/6nq0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nq0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nq0 OCA], [http://pdbe.org/6nq0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nq0 RCSB], [http://www.ebi.ac.uk/pdbsum/6nq0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nq0 ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 6nq0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6nq0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bai, X]]		[[Category: Bai, X]]

OCA at 06:45, 27 March 2019

OCA — Wed, 27 Mar 2019 06:45:05 GMT

←Older revision		Revision as of 06:45, 27 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6nq0 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Cryo-EM structure of human TPC2 channel in the ligand-bound open state==
		+	<StructureSection load='6nq0' size='340' side='right'caption='[[6nq0]], [[Resolution\|resolution]] 3.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6nq0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NQ0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EUJ:(2R)-3-{[(S)-hydroxy{[(1S,2R,3R,4S,5S,6R)-2,4,6-trihydroxy-3,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl+dioctanoate'>EUJ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nq0 OCA], [http://pdbe.org/6nq0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nq0 RCSB], [http://www.ebi.ac.uk/pdbsum/6nq0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nq0 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TPC2_HUMAN TPC2_HUMAN]] Nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal membrane. May be involved in smooth muscle contraction.<ref>PMID:19387438</ref> <ref>PMID:19620632</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mammalian two-pore channels (TPCs) regulate the physiological functions of the endolysosome. Here we present cryo-EM structures of human TPC2 (HsTPC2), a phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2)-activated, Na(+) selective channel, in the ligand-bound and apo states. The apo structure captures the closed conformation, while the ligand-bound form features the channel in both open and closed conformations. Combined with functional analysis, these structures provide insights into the mechanism of PI(3,5)P2-regulated gating of TPC2, which is distinct from that of TPC1. Specifically, the endolysosome-specific PI(3,5)P2 binds at the first 6-TM and activates the channel - independently of the membrane potential - by inducing a structural change at the pore-lining inner helix (IS6), which forms a continuous helix in the open state but breaks into two segments at Gly317 in the closed state. Additionally, structural comparison to the voltage-dependent TPC1 structure allowed us to identify Ile551 as being responsible for the loss of voltage dependence in TPC2.

-	~~Authors~~:	+	Structural mechanisms of phospholipid activation of the human TPC2 channel.,She J, Zeng W, Guo J, Chen Q, Bai XC, Jiang Y Elife. 2019 Mar 12;8. pii: 45222. doi: 10.7554/eLife.45222. PMID:30860481<ref>PMID:30860481</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6nq0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bai, X]]
		+	[[Category: Chen, Q]]
		+	[[Category: Guo, J]]
		+	[[Category: Jiang, Y]]
		+	[[Category: She, J]]
		+	[[Category: Zeng, W]]
		+	[[Category: Channel]]
		+	[[Category: Lysosome]]
		+	[[Category: Transport protein]]

OCA at 05:36, 6 March 2019

OCA — Wed, 06 Mar 2019 05:36:21 GMT

←Older revision		Revision as of 05:36, 6 March 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6nq0 is ON HOLD	+	The entry 6nq0 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 05:06, 21 February 2019

OCA — Thu, 21 Feb 2019 05:06:36 GMT

←Older revision		Revision as of 05:06, 21 February 2019
Line 3:		Line 3:
	The entry 6nq0 is ON HOLD		The entry 6nq0 is ON HOLD

-	Authors: ~~She, J., Zeng, W.Z., Guo, J.T., Chen, Q.F., Bai, X.C., Jiang, Y.X.~~	+	Authors:

-	Description: ~~Cryo-EM structure of human TPC2 channel in the ligand-bound open state~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: She, J]]
-	[[Category: Jiang, Y.X]]
-	[[Category: Bai, X.C]]
-	[[Category: Chen, Q.F]]
-	[[Category: Zeng, W.Z]]
-	[[Category: Guo, J.T]]

OCA: Protected "6nq0" [edit=sysop:move=sysop]

OCA — Wed, 30 Jan 2019 06:44:38 GMT

Protected "6nq0" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:44, 30 January 2019

OCA: New page: '''Unreleased structure''' The entry 6nq0 is ON HOLD Authors: She, J., Zeng, W.Z., Guo, J.T., Chen, Q.F., Bai, X.C., Jiang, Y.X. Description: Cryo-EM structure of human TPC2 channel in...

OCA — Wed, 30 Jan 2019 06:44:36 GMT

New page: '''Unreleased structure''' The entry 6nq0 is ON HOLD Authors: She, J., Zeng, W.Z., Guo, J.T., Chen, Q.F., Bai, X.C., Jiang, Y.X. Description: Cryo-EM structure of human TPC2 channel in...

New page

'''Unreleased structure'''

The entry 6nq0 is ON HOLD

Authors: She, J., Zeng, W.Z., Guo, J.T., Chen, Q.F., Bai, X.C., Jiang, Y.X.

Description: Cryo-EM structure of human TPC2 channel in the ligand-bound open state
[[Category: Unreleased Structures]]
[[Category: She, J]]
[[Category: Jiang, Y.X]]
[[Category: Bai, X.C]]
[[Category: Chen, Q.F]]
[[Category: Zeng, W.Z]]
[[Category: Guo, J.T]]