6nts - Revision history

OCA at 10:33, 15 November 2023

OCA — Wed, 15 Nov 2023 10:33:33 GMT

←Older revision		Revision as of 10:33, 15 November 2023
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	<StructureSection load='6nts' size='340' side='right'caption='[[6nts]], [[Resolution\|resolution]] 3.63Å' scene=''>		<StructureSection load='6nts' size='340' side='right'caption='[[6nts]], [[Resolution\|resolution]] 3.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nts]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NTS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6NTS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nts]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NTS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NTS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=L2J:N-[(1R,2R,3S)-2-hydroxy-3-(10H-phenoxazin-10-yl)cyclohexyl]-4-(trifluoromethoxy)benzene-1-sulfonamide'>L2J</scene>, ~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.63Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=MLL:METHYL+L-LEUCINATE'>MLL</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=L2J:N-[(1R,2R,3S)-2-hydroxy-3-(10H-phenoxazin-10-yl)cyclohexyl]-4-(trifluoromethoxy)benzene-1-sulfonamide'>L2J</scene>, <scene name='pdbligand=MLL:METHYL+L-LEUCINATE'>MLL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">PPP2R1A~~ (~~[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]~~), PPP2R5A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PPP2CA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nts OCA], [https://pdbe.org/6nts PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nts RCSB], [https://www.ebi.ac.uk/pdbsum/6nts PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nts ProSAT]</span></td></tr>
-	~~<tr id='activity~~'>~~<td class="sblockLbl"><b>Activity:~~</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6nts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nts OCA], [~~http~~://pdbe.org/6nts PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6nts RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6nts PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6nts ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/2AAA_HUMAN 2AAA_HUMAN]] The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.<ref>PMID:16580887</ref> [[http://www.uniprot.org/uniprot/PP2AA_HUMAN PP2AA_HUMAN]] PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.<ref>PMID:9920888</ref> <ref>PMID:10801873</ref> <ref>PMID:22613722</ref> [[http://www.uniprot.org/uniprot/2A5A_HUMAN 2A5A_HUMAN]] The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.	+	[https://www.uniprot.org/uniprot/2AAA_HUMAN 2AAA_HUMAN] The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.<ref>PMID:16580887</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6nts" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6nts" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Protein phosphatase 3D structures\|Protein phosphatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Phosphoprotein phosphatase]]~~	+	[[Category: Huang W]]
-	[[Category: Huang, W]]	+	[[Category: Taylor D]]
-	[[Category: Taylor, D]]	+
-	~~[[Category: Activator]]~~	+
-	~~[[Category: Holoenzyme complex]]~~	+
-	~~[[Category: Hydrolase-activator complex]]~~	+
-	~~[[Category: Phosphatase~~]]	+

OCA at 05:47, 13 May 2020

OCA — Wed, 13 May 2020 05:47:52 GMT

←Older revision		Revision as of 05:47, 13 May 2020
Line 1:		Line 1:

	==Protein Phosphatase 2A (Aalpha-B56alpha-Calpha) holoenzyme in complex with a Small Molecule Activator of PP2A (SMAP)==		==Protein Phosphatase 2A (Aalpha-B56alpha-Calpha) holoenzyme in complex with a Small Molecule Activator of PP2A (SMAP)==
-	<StructureSection load='6nts' size='340' side='right'caption='[[6nts]]' scene=''>	+	<StructureSection load='6nts' size='340' side='right'caption='[[6nts]], [[Resolution\|resolution]] 3.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NTS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NTS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nts]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NTS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NTS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nts OCA], [http://pdbe.org/6nts PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nts RCSB], [http://www.ebi.ac.uk/pdbsum/6nts PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nts ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=L2J:N-[(1R,2R,3S)-2-hydroxy-3-(10H-phenoxazin-10-yl)cyclohexyl]-4-(trifluoromethoxy)benzene-1-sulfonamide'>L2J</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLL:METHYL+L-LEUCINATE'>MLL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PPP2R1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PPP2R5A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PPP2CA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nts OCA], [http://pdbe.org/6nts PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nts RCSB], [http://www.ebi.ac.uk/pdbsum/6nts PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nts ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/2AAA_HUMAN 2AAA_HUMAN]] The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.<ref>PMID:16580887</ref> [[http://www.uniprot.org/uniprot/PP2AA_HUMAN PP2AA_HUMAN]] PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.<ref>PMID:9920888</ref> <ref>PMID:10801873</ref> <ref>PMID:22613722</ref> [[http://www.uniprot.org/uniprot/2A5A_HUMAN 2A5A_HUMAN]] The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Impairment of protein phosphatases, including the family of serine/threonine phosphatases designated PP2A, is essential for the pathogenesis of many diseases, including cancer. The ability of PP2A to dephosphorylate hundreds of proteins is regulated by over 40 specificity-determining regulatory "B" subunits that compete for assembly and activation of heterogeneous PP2A heterotrimers. Here, we reveal how a small molecule, DT-061, specifically stabilizes the B56alpha-PP2A holoenzyme in a fully assembled, active state to dephosphorylate selective substrates, such as its well-known oncogenic target, c-Myc. Our 3.6 A structure identifies molecular interactions between DT-061 and all three PP2A subunits that prevent dissociation of the active enzyme and highlight inherent mechanisms of PP2A complex assembly. Thus, our findings provide fundamental insights into PP2A complex assembly and regulation, identify a unique interfacial stabilizing mode of action for therapeutic targeting, and aid in the development of phosphatase-based therapeutics tailored against disease specific phospho-protein targets.
		+
		+	Selective PP2A Enhancement through Biased Heterotrimer Stabilization.,Leonard D, Huang W, Izadmehr S, O'Connor CM, Wiredja DD, Wang Z, Zaware N, Chen Y, Schlatzer DM, Kiselar J, Vasireddi N, Schuchner S, Perl AL, Galsky MD, Xu W, Brautigan DL, Ogris E, Taylor DJ, Narla G Cell. 2020 Apr 30;181(3):688-701.e16. doi: 10.1016/j.cell.2020.03.038. Epub 2020 , Apr 20. PMID:32315618<ref>PMID:32315618</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6nts" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Huang W]]	+	[[Category: Phosphoprotein phosphatase]]
-	[[Category: Taylor D]]	+	[[Category: Huang, W]]
		+	[[Category: Taylor, D]]
		+	[[Category: Activator]]
		+	[[Category: Holoenzyme complex]]
		+	[[Category: Hydrolase-activator complex]]
		+	[[Category: Phosphatase]]

OCA at 06:00, 6 May 2020

OCA — Wed, 06 May 2020 06:00:18 GMT

←Older revision		Revision as of 06:00, 6 May 2020
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-	'''Unreleased structure'''

-	~~The entry 6nts is ON HOLD until Paper Publication~~	+	==Protein Phosphatase 2A (Aalpha-B56alpha-Calpha) holoenzyme in complex with a Small Molecule Activator of PP2A (SMAP)==
-		+	<StructureSection load='6nts' size='340' side='right'caption='[[6nts]]' scene=''>
-	~~Authors: Huang, W., Taylor, D.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NTS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NTS FirstGlance]. <br>
-	~~Description:~~ Protein Phosphatase 2A (Aalpha-B56alpha-Calpha) holoenzyme in complex with a Small Molecule Activator of PP2A (SMAP)	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nts OCA], [http://pdbe.org/6nts PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nts RCSB], [http://www.ebi.ac.uk/pdbsum/6nts PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nts ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Huang, W]]	+	__TOC__
-	[[Category: Taylor, D]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Huang W]]
		+	[[Category: Taylor D]]

OCA at 05:08, 21 February 2019

OCA — Thu, 21 Feb 2019 05:08:27 GMT

←Older revision		Revision as of 05:08, 21 February 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6nts is ON HOLD	+	The entry 6nts is ON HOLD until Paper Publication

	Authors: Huang, W., Taylor, D.		Authors: Huang, W., Taylor, D.

OCA: Protected "6nts" [edit=sysop:move=sysop]

OCA — Wed, 13 Feb 2019 07:43:52 GMT

Protected "6nts" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:43, 13 February 2019

OCA: New page: '''Unreleased structure''' The entry 6nts is ON HOLD Authors: Huang, W., Taylor, D. Description: Protein Phosphatase 2A (Aalpha-B56alpha-Calpha) holoenzyme in complex with a Small Mole...

OCA — Wed, 13 Feb 2019 07:43:50 GMT

New page: '''Unreleased structure''' The entry 6nts is ON HOLD Authors: Huang, W., Taylor, D. Description: Protein Phosphatase 2A (Aalpha-B56alpha-Calpha) holoenzyme in complex with a Small Mole...

New page

'''Unreleased structure'''

The entry 6nts is ON HOLD

Authors: Huang, W., Taylor, D.

Description: Protein Phosphatase 2A (Aalpha-B56alpha-Calpha) holoenzyme in complex with a Small Molecule Activator of PP2A (SMAP)
[[Category: Unreleased Structures]]
[[Category: Huang, W]]
[[Category: Taylor, D]]