6nx4 - Revision history

OCA at 07:36, 1 May 2024

OCA — Wed, 01 May 2024 07:36:31 GMT

←Older revision		Revision as of 07:36, 1 May 2024
Line 1:		Line 1:

	==Structure of the C-terminal Helical Repeat Domain of Eukaryotic Elongation Factor 2 Kinase (eEF-2K)==		==Structure of the C-terminal Helical Repeat Domain of Eukaryotic Elongation Factor 2 Kinase (eEF-2K)==
-	<StructureSection load='6nx4' size='340' side='right'caption='[[6nx4~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='6nx4' size='340' side='right'caption='[[6nx4]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nx4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NX4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6NX4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nx4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NX4 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>EEF2K ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=9606 HUMAN])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Elongation_factor_2]_kinase [Elongation factor 2] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.20 2.7.11.20] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nx4 OCA], [https://pdbe.org/6nx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nx4 RCSB], [https://www.ebi.ac.uk/pdbsum/6nx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nx4 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6nx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nx4 OCA], [~~http~~://pdbe.org/6nx4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6nx4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6nx4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6nx4 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/EF2K_HUMAN EF2K_HUMAN]] Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.<ref>PMID:14709557</ref> <ref>PMID:9144159</ref>	+	[https://www.uniprot.org/uniprot/EF2K_HUMAN EF2K_HUMAN] Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.<ref>PMID:14709557</ref> <ref>PMID:9144159</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Eukaryotic elongation factor 2 kinase (eEF-2K), an atypical calmodulin-activated protein kinase, regulates translational elongation by phosphorylating its substrate, eukaryotic elongation factor 2 (eEF-2), thereby reducing its affinity for the ribosome. The activation and activity of eEF-2K are critical for survival under energy-deprived conditions and is implicated in a variety of essential physiological processes. Previous biochemical experiments have indicated that the binding site for the substrate eEF-2 is located in the C-terminal domain of eEF-2K, a region predicted to harbor several alpha-helical repeats. Here, using NMR methodology we have determined the solution structure of a C-terminal fragment of eEF-2K, eEF-2K562-725 that encodes two alpha-helical repeats. The structure of eEF-2K562-725 shows signatures characteristic of TPR domains and of their SEL1-like sub-family. Further, using the analyses of NMR spectral perturbations and ITC measurements, we have localized the eEF-2 binding site on eEF-2K562-725. We find that eEF-2K562-725 engages eEF-2 with an affinity comparable to that of the full-length enzyme. Further, eEF-2K562-725 is able to inhibit the phosphorylation of eEF-2 by full-length eEF-2K in trans. Our present studies establish that eEF-2K562-725 encodes the major elements necessary to enable the eEF-2K/eEF-2 interactions.	+
-		+
-	Solution Structure of the Carboxy-Terminal Tandem Repeat Domain of Eukaryotic Elongation Factor 2 Kinase and its Role in Substrate Recognition.,Piserchio A, Will N, Giles DH, Hajredini F, Dalby KN, Ghose R J Mol Biol. 2019 May 17. pii: S0022-2836(19)30290-6. doi:, 10.1016/j.jmb.2019.05.019. PMID:31108082<ref>PMID:31108082</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6nx4" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dalby~~, K N~~]]	+	[[Category: Dalby KN]]
-	[[Category: Ghose, R]]	+	[[Category: Ghose R]]
-	[[Category: Giles~~, D H~~]]	+	[[Category: Giles DH]]
-	[[Category: Hajredini, F]]	+	[[Category: Hajredini F]]
-	[[Category: Piserchio, A]]	+	[[Category: Piserchio A]]
-	[[Category: Will, N]]	+	[[Category: Will N]]
-	~~[[Category: Binding domain]]~~	+
-	~~[[Category: Eef2]]~~	+
-	~~[[Category: Eef2k]]~~	+
-	~~[[Category: Elongation]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Sel1]]~~	+
-	~~[[Category: Tpr]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Translation~~]]	+

OCA at 06:29, 10 July 2019

OCA — Wed, 10 Jul 2019 06:29:23 GMT

←Older revision		Revision as of 06:29, 10 July 2019
Line 3:		Line 3:
	<StructureSection load='6nx4' size='340' side='right'caption='[[6nx4]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='6nx4' size='340' side='right'caption='[[6nx4]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nx4]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NX4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nx4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NX4 FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Elongation_factor_2]_kinase [Elongation factor 2] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.20 2.7.11.20] </span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EEF2K ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Elongation_factor_2]_kinase [Elongation factor 2] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.20 2.7.11.20] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nx4 OCA], [http://pdbe.org/6nx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nx4 RCSB], [http://www.ebi.ac.uk/pdbsum/6nx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nx4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nx4 OCA], [http://pdbe.org/6nx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nx4 RCSB], [http://www.ebi.ac.uk/pdbsum/6nx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nx4 ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Dalby, K N]]		[[Category: Dalby, K N]]

OCA at 06:14, 29 May 2019

OCA — Wed, 29 May 2019 06:14:57 GMT

←Older revision		Revision as of 06:14, 29 May 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6nx4 is ~~ON HOLD~~	+	==Structure of the C-terminal Helical Repeat Domain of Eukaryotic Elongation Factor 2 Kinase (eEF-2K)==
		+	<StructureSection load='6nx4' size='340' side='right'caption='[[6nx4]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6nx4]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NX4 FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Elongation_factor_2]_kinase [Elongation factor 2] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.20 2.7.11.20] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nx4 OCA], [http://pdbe.org/6nx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nx4 RCSB], [http://www.ebi.ac.uk/pdbsum/6nx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nx4 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/EF2K_HUMAN EF2K_HUMAN]] Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.<ref>PMID:14709557</ref> <ref>PMID:9144159</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Eukaryotic elongation factor 2 kinase (eEF-2K), an atypical calmodulin-activated protein kinase, regulates translational elongation by phosphorylating its substrate, eukaryotic elongation factor 2 (eEF-2), thereby reducing its affinity for the ribosome. The activation and activity of eEF-2K are critical for survival under energy-deprived conditions and is implicated in a variety of essential physiological processes. Previous biochemical experiments have indicated that the binding site for the substrate eEF-2 is located in the C-terminal domain of eEF-2K, a region predicted to harbor several alpha-helical repeats. Here, using NMR methodology we have determined the solution structure of a C-terminal fragment of eEF-2K, eEF-2K562-725 that encodes two alpha-helical repeats. The structure of eEF-2K562-725 shows signatures characteristic of TPR domains and of their SEL1-like sub-family. Further, using the analyses of NMR spectral perturbations and ITC measurements, we have localized the eEF-2 binding site on eEF-2K562-725. We find that eEF-2K562-725 engages eEF-2 with an affinity comparable to that of the full-length enzyme. Further, eEF-2K562-725 is able to inhibit the phosphorylation of eEF-2 by full-length eEF-2K in trans. Our present studies establish that eEF-2K562-725 encodes the major elements necessary to enable the eEF-2K/eEF-2 interactions.

-	~~Authors: Piserchio~~, A., Will, N., Giles~~, D.H.~~, Hajredini, F., Dalby, K.N., ~~Ghose, R~~.	+	Solution Structure of the Carboxy-Terminal Tandem Repeat Domain of Eukaryotic Elongation Factor 2 Kinase and its Role in Substrate Recognition.,Piserchio A, Will N, Giles DH, Hajredini F, Dalby KN, Ghose R J Mol Biol. 2019 May 17. pii: S0022-2836(19)30290-6. doi:, 10.1016/j.jmb.2019.05.019. PMID:31108082<ref>PMID:31108082</ref>

-	~~Description: Structure~~ of the ~~C-terminal Helical Repeat Domain~~ of ~~Eukaryotic Elongation Factor 2 Kinase (eEF~~-~~2K)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Piserchio~~, A]]	+	<div class="pdbe-citations 6nx4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Dalby, K N]]
	[[Category: Ghose, R]]		[[Category: Ghose, R]]
-	[[Category: Giles, D.H]]	+	[[Category: Giles, D H]]
-	~~[[Category: Will, N]]~~	+
-	~~[[Category: Dalby, K.N~~]]	+
	[[Category: Hajredini, F]]		[[Category: Hajredini, F]]
		+	[[Category: Piserchio, A]]
		+	[[Category: Will, N]]
		+	[[Category: Binding domain]]
		+	[[Category: Eef2]]
		+	[[Category: Eef2k]]
		+	[[Category: Elongation]]
		+	[[Category: Kinase]]
		+	[[Category: Sel1]]
		+	[[Category: Tpr]]
		+	[[Category: Transferase]]
		+	[[Category: Translation]]

OCA: Protected "6nx4" [edit=sysop:move=sysop]

OCA — Wed, 13 Feb 2019 07:54:45 GMT

Protected "6nx4" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:54, 13 February 2019

OCA: New page: '''Unreleased structure''' The entry 6nx4 is ON HOLD Authors: Piserchio, A., Will, N., Giles, D.H., Hajredini, F., Dalby, K.N., Ghose, R. Description: Structure of the C-terminal Helic...

OCA — Wed, 13 Feb 2019 07:54:43 GMT

New page: '''Unreleased structure''' The entry 6nx4 is ON HOLD Authors: Piserchio, A., Will, N., Giles, D.H., Hajredini, F., Dalby, K.N., Ghose, R. Description: Structure of the C-terminal Helic...

New page

'''Unreleased structure'''

The entry 6nx4 is ON HOLD

Authors: Piserchio, A., Will, N., Giles, D.H., Hajredini, F., Dalby, K.N., Ghose, R.

Description: Structure of the C-terminal Helical Repeat Domain of Eukaryotic Elongation Factor 2 Kinase (eEF-2K)
[[Category: Unreleased Structures]]
[[Category: Piserchio, A]]
[[Category: Ghose, R]]
[[Category: Giles, D.H]]
[[Category: Will, N]]
[[Category: Dalby, K.N]]
[[Category: Hajredini, F]]