6o0z - Revision history

OCA at 09:22, 20 March 2024

OCA — Wed, 20 Mar 2024 09:22:21 GMT

←Older revision		Revision as of 09:22, 20 March 2024
Line 3:		Line 3:
	<SX load='6o0z' size='340' side='right' viewer='molstar' caption='[[6o0z]], [[Resolution\|resolution]] 3.30Å' scene=''>		<SX load='6o0z' size='340' side='right' viewer='molstar' caption='[[6o0z]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6o0z]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O0Z OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6O0Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6o0z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O0Z FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~cas9~~, ~~csn1, SPy_1046 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=1314 "Micrococcus scarlatinae" Klein 1884])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.3Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6o0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o0z OCA], [~~http~~://pdbe.org/6o0z PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6o0z RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6o0z PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6o0z ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o0z OCA], [https://pdbe.org/6o0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o0z RCSB], [https://www.ebi.ac.uk/pdbsum/6o0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o0z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref>	+	[https://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The RNA-guided Cas9 endonuclease from Streptococcus pyogenes is a single-turnover enzyme that displays a stable product state after double-stranded-DNA cleavage. Here, we present cryo-EM structures of precatalytic, postcatalytic and product states of the active Cas9-sgRNA-DNA complex in the presence of Mg(2+). In the precatalytic state, Cas9 adopts the 'checkpoint' conformation with the HNH nuclease domain positioned far away from the DNA. Transition to the postcatalytic state involves a dramatic ~34-A swing of the HNH domain and disorder of the REC2 recognition domain. The postcatalytic state captures the cleaved substrate bound to the catalytically competent HNH active site. In the product state, the HNH domain is disordered, REC2 returns to the precatalytic conformation, and additional interactions of REC3 and RuvC with nucleic acids are formed. The coupled domain motions and interactions between the enzyme and the RNA-DNA hybrid provide new insights into the mechanism of genome editing by Cas9.	+

-	Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9.,Zhu X, Clarke R, Puppala AK, Chittori S, Merk A, Merrill BJ, Simonovic M, Subramaniam S Nat Struct Mol Biol. 2019 Jul 8. pii: 10.1038/s41594-019-0258-2. doi:, 10.1038/s41594-019-0258-2. PMID:31285607<ref>PMID:31285607</ref>	+	==See Also==
-		+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 6o0z" style~~=~~"background-color:#fffaf0;"></div>~~	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: Micrococcus scarlatinae klein 1884]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chittori, S~~]]	+	[[Category: Streptococcus pyogenes]]
-	[[Category: ~~Clarke, R~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Merk, A~~]]	+	[[Category: Chittori S]]
-	[[Category: ~~Merrill, B J~~]]	+	[[Category: Clarke R]]
-	[[Category: ~~Puppala,~~ A K]]	+	[[Category: Merk A]]
-	[[Category: ~~Simonovic, M~~]]	+	[[Category: Merrill BJ]]
-	[[Category: ~~Subramaniam, S~~]]	+	[[Category: Puppala AK]]
-	[[Category: ~~Zhu, X~~]]	+	[[Category: Simonovic M]]
-	[[Category: ~~Cas9~~]]	+	[[Category: Subramaniam S]]
-	[[Category: ~~Crispr]]~~	+	[[Category: Zhu X]]
-	~~[[Category: Genome editing]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-rna-dna complex]]~~	+
-	~~[[Category: Nuclease~~]]	+

OCA at 03:40, 11 April 2020

OCA — Sat, 11 Apr 2020 03:40:52 GMT

←Older revision		Revision as of 03:40, 11 April 2020
Line 3:		Line 3:
	<SX load='6o0z' size='340' side='right' viewer='molstar' caption='[[6o0z]], [[Resolution\|resolution]] 3.30Å' scene=''>		<SX load='6o0z' size='340' side='right' viewer='molstar' caption='[[6o0z]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6o0z]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O0Z OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6O0Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6o0z]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O0Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6O0Z FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cas9, csn1, SPy_1046 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1314 "Micrococcus scarlatinae" Klein 1884])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cas9, csn1, SPy_1046 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1314 "Micrococcus scarlatinae" Klein 1884])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6o0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o0z OCA], [http://pdbe.org/6o0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o0z RCSB], [http://www.ebi.ac.uk/pdbsum/6o0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o0z ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6o0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o0z OCA], [http://pdbe.org/6o0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o0z RCSB], [http://www.ebi.ac.uk/pdbsum/6o0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o0z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 22:58, 6 March 2020

OCA — Fri, 06 Mar 2020 22:58:35 GMT

←Older revision		Revision as of 22:58, 6 March 2020
Line 1:		Line 1:

	==Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium==		==Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium==
-	<~~StructureSection~~ load='6o0z' size='340' side='right'caption='[[6o0z]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<SX load='6o0z' size='340' side='right' viewer='molstar' caption='[[6o0z]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6o0z]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O0Z FirstGlance]. <br>		<table><tr><td colspan='2'>[[6o0z]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O0Z FirstGlance]. <br>
Line 21:		Line 21:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Micrococcus scarlatinae klein 1884]]		[[Category: Micrococcus scarlatinae klein 1884]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 08:40, 24 July 2019

OCA — Wed, 24 Jul 2019 08:40:20 GMT

←Older revision		Revision as of 08:40, 24 July 2019
Line 3:		Line 3:
	<StructureSection load='6o0z' size='340' side='right'caption='[[6o0z]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='6o0z' size='340' side='right'caption='[[6o0z]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6o0z]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O0Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6o0z]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O0Z FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o0z OCA], [http://pdbe.org/6o0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o0z RCSB], [http://www.ebi.ac.uk/pdbsum/6o0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o0z ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cas9, csn1, SPy_1046 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1314 "Micrococcus scarlatinae" Klein 1884])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o0z OCA], [http://pdbe.org/6o0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o0z RCSB], [http://www.ebi.ac.uk/pdbsum/6o0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o0z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref>		[[http://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The RNA-guided Cas9 endonuclease from Streptococcus pyogenes is a single-turnover enzyme that displays a stable product state after double-stranded-DNA cleavage. Here, we present cryo-EM structures of precatalytic, postcatalytic and product states of the active Cas9-sgRNA-DNA complex in the presence of Mg(2+). In the precatalytic state, Cas9 adopts the 'checkpoint' conformation with the HNH nuclease domain positioned far away from the DNA. Transition to the postcatalytic state involves a dramatic ~34-A swing of the HNH domain and disorder of the REC2 recognition domain. The postcatalytic state captures the cleaved substrate bound to the catalytically competent HNH active site. In the product state, the HNH domain is disordered, REC2 returns to the precatalytic conformation, and additional interactions of REC3 and RuvC with nucleic acids are formed. The coupled domain motions and interactions between the enzyme and the RNA-DNA hybrid provide new insights into the mechanism of genome editing by Cas9.
		+
		+	Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9.,Zhu X, Clarke R, Puppala AK, Chittori S, Merk A, Merrill BJ, Simonovic M, Subramaniam S Nat Struct Mol Biol. 2019 Jul 8. pii: 10.1038/s41594-019-0258-2. doi:, 10.1038/s41594-019-0258-2. PMID:31285607<ref>PMID:31285607</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6o0z" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Micrococcus scarlatinae klein 1884]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chittori, S]]		[[Category: Chittori, S]]

OCA at 05:48, 10 July 2019

OCA — Wed, 10 Jul 2019 05:48:43 GMT

←Older revision		Revision as of 05:48, 10 July 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6o0z is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium==
-		+	<StructureSection load='6o0z' size='340' side='right'caption='[[6o0z]], [[Resolution\|resolution]] 3.30Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6o0z]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O0Z FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o0z OCA], [http://pdbe.org/6o0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o0z RCSB], [http://www.ebi.ac.uk/pdbsum/6o0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o0z ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chittori, S]]
		+	[[Category: Clarke, R]]
		+	[[Category: Merk, A]]
		+	[[Category: Merrill, B J]]
		+	[[Category: Puppala, A K]]
		+	[[Category: Simonovic, M]]
		+	[[Category: Subramaniam, S]]
		+	[[Category: Zhu, X]]
		+	[[Category: Cas9]]
		+	[[Category: Crispr]]
		+	[[Category: Genome editing]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase-rna-dna complex]]
		+	[[Category: Nuclease]]

OCA at 03:58, 22 May 2019

OCA — Wed, 22 May 2019 03:58:09 GMT

←Older revision		Revision as of 03:58, 22 May 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6o0z is ON HOLD	+	The entry 6o0z is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6o0z" [edit=sysop:move=sysop]

OCA — Wed, 27 Feb 2019 13:48:37 GMT

Protected "6o0z" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:48, 27 February 2019

OCA: New page: '''Unreleased structure''' The entry 6o0z is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Feb 2019 13:48:36 GMT

New page: '''Unreleased structure''' The entry 6o0z is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6o0z is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]