6o2w - Revision history

OCA at 07:03, 11 October 2023

OCA — Wed, 11 Oct 2023 07:03:24 GMT

←Older revision		Revision as of 07:03, 11 October 2023
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	<StructureSection load='6o2w' size='340' side='right'caption='[[6o2w]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6o2w' size='340' side='right'caption='[[6o2w]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6o2w]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O2W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6O2W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6o2w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O2W FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.101Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6o2u\|6o2u]]~~, [[~~6o2v~~\|~~6o2v~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~SARAF, TMEM66, XTP3, HSPC035, NPD003, PSEC0019, UNQ1967/PRO4499 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o2w OCA], [https://pdbe.org/6o2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o2w RCSB], [https://www.ebi.ac.uk/pdbsum/6o2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o2w ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6o2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o2w OCA], [~~http~~://pdbe.org/6o2w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6o2w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6o2w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6o2w ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SARAF_HUMAN SARAF_HUMAN]] Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.<ref>PMID:22464749</ref>	+	[https://www.uniprot.org/uniprot/SARAF_HUMAN SARAF_HUMAN] Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.<ref>PMID:22464749</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kimberlin~~, C R~~]]	+	[[Category: Kimberlin CR]]
-	[[Category: Minor~~, D L]]~~	+	[[Category: Minor DL]]
-	~~[[Category: Calcium signaling]]~~	+
-	~~[[Category: Domain swap]]~~	+
-	~~[[Category: Endoplasmic reticulum]]~~	+
-	~~[[Category: Er]]~~	+
-	~~[[Category: Saraf]]~~	+
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Soce]]~~	+
-	~~[[Category: Store operated calcium entry~~]]	+

OCA at 06:36, 19 June 2019

OCA — Wed, 19 Jun 2019 06:36:11 GMT

←Older revision		Revision as of 06:36, 19 June 2019
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	<StructureSection load='6o2w' size='340' side='right'caption='[[6o2w]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6o2w' size='340' side='right'caption='[[6o2w]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6o2w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O2W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6o2w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O2W FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6o2u\|6o2u]], [[6o2v\|6o2v]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6o2u\|6o2u]], [[6o2v\|6o2v]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SARAF, TMEM66, XTP3, HSPC035, NPD003, PSEC0019, UNQ1967/PRO4499 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o2w OCA], [http://pdbe.org/6o2w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o2w RCSB], [http://www.ebi.ac.uk/pdbsum/6o2w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o2w ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o2w OCA], [http://pdbe.org/6o2w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o2w RCSB], [http://www.ebi.ac.uk/pdbsum/6o2w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o2w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SARAF_HUMAN SARAF_HUMAN]] Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.<ref>PMID:22464749</ref>		[[http://www.uniprot.org/uniprot/SARAF_HUMAN SARAF_HUMAN]] Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.<ref>PMID:22464749</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Store-Operated Calcium Entry (SOCE) plays key roles in cell proliferation, muscle contraction, immune responses, and memory formation. The coordinated interactions of a number of proteins from the plasma and endoplasmic reticulum membranes control SOCE to replenish internal Ca(2+) stores and generate intracellular Ca(2+) signals. SARAF, an endoplasmic reticulum resident component of the SOCE pathway having no homology to any characterized protein, serves as an important brake on SOCE. Here, we describe the X-ray crystal structure of the SARAF luminal domain, SARAFL. This domain forms a novel 10-stranded beta-sandwich fold that includes a set of three conserved disulfide bonds, denoted the "SARAF-fold." The structure reveals a domain-swapped dimer in which the last two beta-strands (beta9 and beta10) are exchanged forming a region denoted the "SARAF luminal switch" that is essential for dimerization. Sequence comparisons reveal that the SARAF-fold is highly conserved in vertebrates and in a variety of pathologic fungi. Forster resonance energy transfer experiments using full-length SARAF validate the formation of the domain-swapped dimer in cells and demonstrate that dimerization is reversible. A designed variant lacking the SARAF luminal switch shows that the domain swapping is essential to function and indicates that the SARAF dimer accelerates SOCE inactivation.
		+
		+	SARAF Luminal Domain Structure Reveals a Novel Domain-Swapped beta-Sandwich Fold Important for SOCE Modulation.,Kimberlin CR, Meshcheriakova A, Palty R, Raveh A, Karbat I, Reuveny E, Minor DL Jr J Mol Biol. 2019 May 11. pii: S0022-2836(19)30263-3. doi:, 10.1016/j.jmb.2019.05.008. PMID:31082439<ref>PMID:31082439</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6o2w" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Kimberlin, C R]]		[[Category: Kimberlin, C R]]

OCA at 06:15, 29 May 2019

OCA — Wed, 29 May 2019 06:15:41 GMT

←Older revision		Revision as of 06:15, 29 May 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6o2w is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the SARAF luminal domain Cys-lock mutant dimer==
-		+	<StructureSection load='6o2w' size='340' side='right'caption='[[6o2w]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6o2w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O2W FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6o2u\|6o2u]], [[6o2v\|6o2v]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o2w OCA], [http://pdbe.org/6o2w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o2w RCSB], [http://www.ebi.ac.uk/pdbsum/6o2w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o2w ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SARAF_HUMAN SARAF_HUMAN]] Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.<ref>PMID:22464749</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kimberlin, C R]]
		+	[[Category: Minor, D L]]
		+	[[Category: Calcium signaling]]
		+	[[Category: Domain swap]]
		+	[[Category: Endoplasmic reticulum]]
		+	[[Category: Er]]
		+	[[Category: Saraf]]
		+	[[Category: Signaling protein]]
		+	[[Category: Soce]]
		+	[[Category: Store operated calcium entry]]

OCA at 04:58, 27 March 2019

OCA — Wed, 27 Mar 2019 04:58:21 GMT

←Older revision		Revision as of 04:58, 27 March 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6o2w is ON HOLD	+	The entry 6o2w is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6o2w" [edit=sysop:move=sysop]

OCA — Wed, 06 Mar 2019 05:42:14 GMT

Protected "6o2w" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:42, 6 March 2019

OCA: New page: '''Unreleased structure''' The entry 6o2w is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 06 Mar 2019 05:42:12 GMT

New page: '''Unreleased structure''' The entry 6o2w is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6o2w is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]