6o9w - Revision history

OCA at 07:06, 11 October 2023

OCA — Wed, 11 Oct 2023 07:06:37 GMT

←Older revision		Revision as of 07:06, 11 October 2023
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	<StructureSection load='6o9w' size='340' side='right'caption='[[6o9w]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='6o9w' size='340' side='right'caption='[[6o9w]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6o9w]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O9W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6O9W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6o9w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O9W FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NXL:(2S~~,~~5R)-1-FORMYL-5-~~[~~(SULFOOXY)AMINO~~]~~PIPERIDINE-2-CARBOXAMIDE'>NXL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>blaR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1280~~ "~~Micrococcus aureus~~" (~~Rosenbach 1884~~) ~~Zopf 1885]~~)</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NXL:(2S,5R)-1-FORMYL-5-[(SULFOOXY)AMINO]PIPERIDINE-2-CARBOXAMIDE'>NXL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6o9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o9w OCA], [~~http~~://pdbe.org/6o9w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6o9w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6o9w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6o9w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o9w OCA], [https://pdbe.org/6o9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o9w RCSB], [https://www.ebi.ac.uk/pdbsum/6o9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o9w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/BLAR_STAAU BLAR_STAAU]] BlaR1 is a potential penicillin-binding protein required for induction of beta-lactamase.	+	[https://www.uniprot.org/uniprot/BLAR_STAAU BLAR_STAAU] BlaR1 is a potential penicillin-binding protein required for induction of beta-lactamase.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Alexander, J A.N~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Strynadka, N C.J~~]]	+	[[Category: Alexander JAN]]
-	[[Category: ~~Antibiotic complex]]~~	+	[[Category: Strynadka NCJ]]
-	~~[[Category: Beta-lactam antibiotic sensor domain]]~~	+
-	~~[[Category: Penicillin binding protein]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 06:30, 7 October 2020

OCA — Wed, 07 Oct 2020 06:30:48 GMT

←Older revision		Revision as of 06:30, 7 October 2020
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	==Crystal structure of Staphylococcus aureus BlaR1 antibiotic-sensor domain in complex with avibactam==		==Crystal structure of Staphylococcus aureus BlaR1 antibiotic-sensor domain in complex with avibactam==
-	<StructureSection load='6o9w' size='340' side='right'caption='[[6o9w]]' scene=''>	+	<StructureSection load='6o9w' size='340' side='right'caption='[[6o9w]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O9W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6O9W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6o9w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O9W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6O9W FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6o9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o9w OCA], [http://pdbe.org/6o9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o9w RCSB], [http://www.ebi.ac.uk/pdbsum/6o9w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o9w ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NXL:(2S,5R)-1-FORMYL-5-[(SULFOOXY)AMINO]PIPERIDINE-2-CARBOXAMIDE'>NXL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">blaR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6o9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o9w OCA], [http://pdbe.org/6o9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o9w RCSB], [http://www.ebi.ac.uk/pdbsum/6o9w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o9w ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BLAR_STAAU BLAR_STAAU]] BlaR1 is a potential penicillin-binding protein required for induction of beta-lactamase.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Methicillin-resistant Staphylococcus aureus (MRSA) infections cause significant mortality and morbidity globally. MRSA resistance to beta-lactam antibiotics is mediated by two divergons that control levels of a beta-lactamase, PC1, and a penicillin-binding protein poorly acylated by beta-lactam antibiotics, PBP2a. Expression of genes encoding these proteins is controlled by two integral membrane proteins, BlaR1 and MecR1, which both have an extracellular beta-lactam-binding sensor domain. Here, we solved the X-ray crystallographic structures of the BlaR1 and MecR1 sensor domains in complex with avibactam, a diazabicyclooctane beta-lactamase inhibitor at 1.6-2.0 A resolution. Additionally, we show that S. aureus SF8300, a clinically relevant strain from the USA300 clone of MRSA, responds to avibactam by up-regulating the expression of the blaZ and pbp2a antibiotic-resistance genes, encoding PC1 and PBP2a, respectively. The BlaR1-avibactam structure of the carbamoyl-enzyme intermediate revealed that avibactam is bound to the active-site serine in two orientations approximately 180 degrees to each other. Although a physiological role of the observed alternative pose remains to be validated, our structural results hint at the presence of a secondary sulfate-binding pocket that could be exploited in the design of future inhibitors of BlaR1/MecR1 sensor domains or the structurally similar class D beta-lactamases. The MecR1-avibactam structure adopted a singular avibactam orientation similar to one of the two states observed in the BlaR1-avibactam structure. Given avibactam up-regulates expression of blaZ and pbp2a antibiotic resistance genes, we suggest further consideration and research is needed to explore what effects administering beta-lactam-avibactam combinations have on treating MRSA infections.
		+
		+	Structural analysis of avibactam-mediated activation of the bla and mec divergons in methicillin-resistant Staphylococcus aureus.,Alexander JAN, Radaeva M, King DT, Chambers HF, Cherkasov A, Chatterjee SS, Strynadka NCJ J Biol Chem. 2020 Aug 7;295(32):10870-10884. doi: 10.1074/jbc.RA120.013029. Epub , 2020 Jun 9. PMID:32518158<ref>PMID:32518158</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6o9w" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alexander ~~JAN~~]]	+	[[Category: Alexander, J A.N]]
-	[[Category: Strynadka ~~NCJ~~]]	+	[[Category: Strynadka, N C.J]]
		+	[[Category: Antibiotic complex]]
		+	[[Category: Beta-lactam antibiotic sensor domain]]
		+	[[Category: Penicillin binding protein]]
		+	[[Category: Signaling protein]]

OCA at 06:23, 10 June 2020

OCA — Wed, 10 Jun 2020 06:23:54 GMT

←Older revision		Revision as of 06:23, 10 June 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6o9w ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of Staphylococcus aureus BlaR1 antibiotic-sensor domain in complex with avibactam==
-		+	<StructureSection load='6o9w' size='340' side='right'caption='[[6o9w]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O9W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6O9W FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6o9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o9w OCA], [http://pdbe.org/6o9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o9w RCSB], [http://www.ebi.ac.uk/pdbsum/6o9w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o9w ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Alexander JAN]]
		+	[[Category: Strynadka NCJ]]

OCA at 05:28, 10 April 2019

OCA — Wed, 10 Apr 2019 05:28:02 GMT

←Older revision		Revision as of 05:28, 10 April 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6o9w is ON HOLD	+	The entry 6o9w is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6o9w" [edit=sysop:move=sysop]

OCA — Wed, 20 Mar 2019 06:04:40 GMT

Protected "6o9w" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:04, 20 March 2019

OCA: New page: '''Unreleased structure''' The entry 6o9w is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 Mar 2019 06:04:38 GMT

New page: '''Unreleased structure''' The entry 6o9w is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6o9w is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]