OCA at 10:40, 31 March 2021

OCA — Wed, 31 Mar 2021 10:40:36 GMT

←Older revision		Revision as of 10:40, 31 March 2021
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	==Structure of Plasmodium falciparum vaccine candidate Pfs230D1M in complex with the Fab of a transmission blocking antibody==		==Structure of Plasmodium falciparum vaccine candidate Pfs230D1M in complex with the Fab of a transmission blocking antibody==
-	<StructureSection load='6ohg' size='340' side='right'caption='[[6ohg]]' scene=''>	+	<StructureSection load='6ohg' size='340' side='right'caption='[[6ohg]], [[Resolution\|resolution]] 2.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OHG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6OHG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ohg]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [https://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OHG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OHG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6ohg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ohg OCA], [~~http~~://pdbe.org/6ohg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ohg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ohg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ohg ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MOH:METHANOL'>MOH</scene>, <scene name='pdbligand=PDO:1,3-PROPANDIOL'>PDO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PFS230, PF230, S230 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 PLAFA])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ohg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ohg OCA], [https://pdbe.org/6ohg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ohg RCSB], [https://www.ebi.ac.uk/pdbsum/6ohg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ohg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/P230_PLAF7 P230_PLAF7]] Gametocyte surface protein required for male/female gamete fusion. Also required for male gamete exflagellation and interaction with erythrocytes.<ref>PMID:16879650</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proteins Pfs230 and Pfs48/45 are Plasmodium falciparum transmission-blocking (TB) vaccine candidates that form a membrane-bound protein complex on gametes. The biological role of Pfs230 or the Pfs230-Pfs48/45 complex remains poorly understood. Here, we present the crystal structure of recombinant Pfs230 domain 1 (Pfs230D1M), a 6-cysteine domain, in complex with the Fab fragment of a TB monoclonal antibody (mAb) 4F12. We observed the arrangement of Pfs230 on the surface of macrogametes differed from that on microgametes, and that Pfs230, with no known membrane anchor, may exist on the membrane surface in the absence of Pfs48/45. 4F12 appears to sterically interfere with Pfs230 function. Combining mAbs against different epitopes of Pfs230D1 or of Pfs230D1 and Pfs48/45, significantly increased TB activity. These studies elucidate a mechanism of action of the Pfs230D1 vaccine, model the functional activity induced by a polyclonal antibody response and support the development of TB vaccines targeting Pfs230D1 and Pfs230D1-Pfs48/45.
		+
		+	Structure and function of a malaria transmission blocking vaccine targeting Pfs230 and Pfs230-Pfs48/45 proteins.,Singh K, Burkhardt M, Nakuchima S, Herrera R, Muratova O, Gittis AG, Kelnhofer E, Reiter K, Smelkinson M, Veltri D, Swihart BJ, Shimp R Jr, Nguyen V, Zhang B, MacDonald NJ, Duffy PE, Garboczi DN, Narum DL Commun Biol. 2020 Jul 24;3(1):395. doi: 10.1038/s42003-020-01123-9. PMID:32709983<ref>PMID:32709983</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ohg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Garboczi DN]]	+	[[Category: Lk3 transgenic mice]]
-	[[Category: Gittis AG]]	+	[[Category: Plafa]]
-	[[Category: Singh K]]	+	[[Category: Garboczi, D N]]
		+	[[Category: Gittis, A G]]
		+	[[Category: Singh, K]]
		+	[[Category: Cysteine-rich pfs230]]
		+	[[Category: Immune system]]
		+	[[Category: Malaria]]
		+	[[Category: Sexual stage]]
		+	[[Category: Transmission blocking]]

OCA at 10:08, 17 June 2020

OCA — Wed, 17 Jun 2020 10:08:56 GMT

←Older revision		Revision as of 10:08, 17 June 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6ohg is ~~ON HOLD until Paper Publication~~	+	==Structure of Plasmodium falciparum vaccine candidate Pfs230D1M in complex with the Fab of a transmission blocking antibody==
-		+	<StructureSection load='6ohg' size='340' side='right'caption='[[6ohg]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OHG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OHG FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ohg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ohg OCA], [http://pdbe.org/6ohg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ohg RCSB], [http://www.ebi.ac.uk/pdbsum/6ohg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ohg ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Garboczi DN]]
		+	[[Category: Gittis AG]]
		+	[[Category: Singh K]]

OCA at 04:21, 16 October 2019

OCA — Wed, 16 Oct 2019 04:21:17 GMT

←Older revision		Revision as of 04:21, 16 October 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ohg is ON HOLD	+	The entry 6ohg is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6ohg" [edit=sysop:move=sysop]

OCA — Wed, 17 Apr 2019 04:09:38 GMT

Protected "6ohg" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:09, 17 April 2019

OCA: New page: '''Unreleased structure''' The entry 6ohg is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 17 Apr 2019 04:09:36 GMT

New page: '''Unreleased structure''' The entry 6ohg is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ohg is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6ohg - Revision history

OCA at 10:40, 31 March 2021

OCA at 10:08, 17 June 2020

OCA at 04:21, 16 October 2019

OCA: Protected "6ohg" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6ohg is ON HOLD Authors: Description: Category: Unreleased Structures