6ohk - Revision history

OCA at 14:52, 13 March 2024

OCA — Wed, 13 Mar 2024 14:52:04 GMT

←Older revision		Revision as of 14:52, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6ohk' size='340' side='right'caption='[[6ohk]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='6ohk' size='340' side='right'caption='[[6ohk]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ohk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_fusiformis"_veillon_and_zuber_1898 "bacillus fusiformis" veillon and zuber 1898~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OHK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6OHK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ohk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusobacterium_nucleatum Fusobacterium nucleatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OHK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.2Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ohk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ohk OCA], [~~http~~://pdbe.org/6ohk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ohk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ohk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ohk ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ohk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ohk OCA], [https://pdbe.org/6ohk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ohk RCSB], [https://www.ebi.ac.uk/pdbsum/6ohk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ohk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/Q8RFH4_FUSNN Q8RFH4_FUSNN]] Low-potential electron donor to a number of redox enzymes.[PIRNR:PIRNR038996]	+	[https://www.uniprot.org/uniprot/Q8RFH4_FUSNN Q8RFH4_FUSNN] Low-potential electron donor to a number of redox enzymes.[PIRNR:PIRNR038996]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Flavodoxins are small FMN-containing proteins that mediate a variety of electron transfer processes. The primary sequence of flavodoxin from Fusobacterium nucleatum, a pathogenic oral bacterium, is marked with a number of distinct features including a glycine to lysine (K13) substitution in the highly conserved phosphate binding loop (T/S-X-T-G-X-T), variation in the aromatic residues that sandwich the FMN cofactor, and a more even distribution of acidic and basic residues. The Eox/sq (oxidized/semiquinone; -43 mV) and Esq/hq (semiquinone/hydroquinone; -256 mV) are the highest recorded reduction potentials of known long-chain flavodoxins. These more electropositive values are a consequence of the apoprotein binding to the FMN hydroquinone anion with ~70-fold greater affinity compared to the oxidized form of the cofactor. Inspection of the FnFld crystal structure revealed the absence of a hydrogen bond between the protein and the oxidized FMN N5 atom, which likely accounts for the more electropositive Eox/sq . The more electropositive Esq/hq is likely attributed to only one negatively charged group positioned within 12 a of the FMN N1. We show that natural substitutions of highly conserved residues partially account for these more electropositive reduction potentials. This article is protected by copyright. All rights reserved.	+
-		+
-	Structural insight into the high reduction potentials observed for Fusobacterium nucleatum flavodoxin.,Mothersole RG, MacDonald M, Kolesnikov M, Murphy MEP, Wolthers KR Protein Sci. 2019 May 22. doi: 10.1002/pro.3661. PMID:31116469<ref>PMID:31116469</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6ohk" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Flavodoxin 3D structures\|Flavodoxin 3D structures]]		*[[Flavodoxin 3D structures\|Flavodoxin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus fusiformis veillon and zuber 1898~~]]	+	[[Category: Fusobacterium nucleatum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kolesnikov, M]]	+	[[Category: Kolesnikov M]]
-	[[Category: Murphy~~, M E.P]]~~	+	[[Category: Murphy MEP]]
-	~~[[Category: Electron transfer]]~~	+
-	~~[[Category: Electron transport]]~~	+
-	~~[[Category: Flavin mononucleotide]]~~	+
-	~~[[Category: Flavodoxin]]~~	+
-	~~[[Category: Reduction potential~~]]	+

OCA at 08:41, 24 July 2019

OCA — Wed, 24 Jul 2019 08:41:12 GMT

←Older revision		Revision as of 08:41, 24 July 2019
Line 3:		Line 3:
	<StructureSection load='6ohk' size='340' side='right'caption='[[6ohk]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='6ohk' size='340' side='right'caption='[[6ohk]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ohk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OHK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ohk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fusiformis"_veillon_and_zuber_1898 "bacillus fusiformis" veillon and zuber 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OHK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ohk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ohk OCA], [http://pdbe.org/6ohk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ohk RCSB], [http://www.ebi.ac.uk/pdbsum/6ohk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ohk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ohk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ohk OCA], [http://pdbe.org/6ohk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ohk RCSB], [http://www.ebi.ac.uk/pdbsum/6ohk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ohk ProSAT]</span></td></tr>
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 6ohk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6ohk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Flavodoxin 3D structures\|Flavodoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus fusiformis veillon and zuber 1898]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Kolesnikov, M]]		[[Category: Kolesnikov, M]]

OCA at 22:51, 5 June 2019

OCA — Wed, 05 Jun 2019 22:51:25 GMT

←Older revision		Revision as of 22:51, 5 June 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6ohk is ~~ON HOLD~~	+	==Crystal structure of Fusobacterium nucleatum flavodoxin mutant K13G bound to flavin mononucleotide==
		+	<StructureSection load='6ohk' size='340' side='right'caption='[[6ohk]], [[Resolution\|resolution]] 1.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ohk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OHK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ohk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ohk OCA], [http://pdbe.org/6ohk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ohk RCSB], [http://www.ebi.ac.uk/pdbsum/6ohk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ohk ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q8RFH4_FUSNN Q8RFH4_FUSNN]] Low-potential electron donor to a number of redox enzymes.[PIRNR:PIRNR038996]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Flavodoxins are small FMN-containing proteins that mediate a variety of electron transfer processes. The primary sequence of flavodoxin from Fusobacterium nucleatum, a pathogenic oral bacterium, is marked with a number of distinct features including a glycine to lysine (K13) substitution in the highly conserved phosphate binding loop (T/S-X-T-G-X-T), variation in the aromatic residues that sandwich the FMN cofactor, and a more even distribution of acidic and basic residues. The Eox/sq (oxidized/semiquinone; -43 mV) and Esq/hq (semiquinone/hydroquinone; -256 mV) are the highest recorded reduction potentials of known long-chain flavodoxins. These more electropositive values are a consequence of the apoprotein binding to the FMN hydroquinone anion with ~70-fold greater affinity compared to the oxidized form of the cofactor. Inspection of the FnFld crystal structure revealed the absence of a hydrogen bond between the protein and the oxidized FMN N5 atom, which likely accounts for the more electropositive Eox/sq . The more electropositive Esq/hq is likely attributed to only one negatively charged group positioned within 12 a of the FMN N1. We show that natural substitutions of highly conserved residues partially account for these more electropositive reduction potentials. This article is protected by copyright. All rights reserved.

-	~~Authors: Kolesnikov~~, M., Murphy, M.E.P.	+	Structural insight into the high reduction potentials observed for Fusobacterium nucleatum flavodoxin.,Mothersole RG, MacDonald M, Kolesnikov M, Murphy MEP, Wolthers KR Protein Sci. 2019 May 22. doi: 10.1002/pro.3661. PMID:31116469<ref>PMID:31116469</ref>

-	~~Description: Crystal structure~~ of ~~Fusobacterium nucleatum flavodoxin mutant K13G bound to flavin mononucleotide~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Murphy, M.E.P~~]]	+	<div class="pdbe-citations 6ohk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Kolesnikov, M]]		[[Category: Kolesnikov, M]]
		+	[[Category: Murphy, M E.P]]
		+	[[Category: Electron transfer]]
		+	[[Category: Electron transport]]
		+	[[Category: Flavin mononucleotide]]
		+	[[Category: Flavodoxin]]
		+	[[Category: Reduction potential]]

OCA: Protected "6ohk" [edit=sysop:move=sysop]

OCA — Wed, 17 Apr 2019 04:10:02 GMT

Protected "6ohk" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:10, 17 April 2019

OCA: New page: '''Unreleased structure''' The entry 6ohk is ON HOLD Authors: Kolesnikov, M., Murphy, M.E.P. Description: Crystal structure of Fusobacterium nucleatum flavodoxin mutant K13G bound to f...

OCA — Wed, 17 Apr 2019 04:10:00 GMT

New page: '''Unreleased structure''' The entry 6ohk is ON HOLD Authors: Kolesnikov, M., Murphy, M.E.P. Description: Crystal structure of Fusobacterium nucleatum flavodoxin mutant K13G bound to f...

New page

'''Unreleased structure'''

The entry 6ohk is ON HOLD

Authors: Kolesnikov, M., Murphy, M.E.P.

Description: Crystal structure of Fusobacterium nucleatum flavodoxin mutant K13G bound to flavin mononucleotide
[[Category: Unreleased Structures]]
[[Category: Murphy, M.E.P]]
[[Category: Kolesnikov, M]]