6ojx - Revision history

OCA at 07:11, 11 October 2023

OCA — Wed, 11 Oct 2023 07:11:53 GMT

←Older revision		Revision as of 07:11, 11 October 2023
Line 3:		Line 3:
	<StructureSection load='6ojx' size='340' side='right'caption='[[6ojx]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='6ojx' size='340' side='right'caption='[[6ojx]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ojx]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Geomg Geomg~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OJX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6OJX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ojx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_metallireducens_GS-15 Geobacter metallireducens GS-15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OJX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.892Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pilT-4, Gmet_1394 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269799 GEOMG])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ojx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ojx OCA], [~~http~~://pdbe.org/6ojx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ojx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ojx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ojx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ojx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ojx OCA], [https://pdbe.org/6ojx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ojx RCSB], [https://www.ebi.ac.uk/pdbsum/6ojx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ojx ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q39VU6_GEOMG Q39VU6_GEOMG]
-	Type IV pilus-like systems are protein complexes that polymerize pilin fibres. They are critical for virulence in many bacterial pathogens. Pilin polymerization and depolymerization are powered by motor ATPases of the PilT/VirB11-like family. This family is thought to operate with C2 symmetry; however, most of these ATPases crystallize with either C3 or C6 symmetric conformations. The relevance of these conformations is unclear. Here, we determine the X-ray structures of PilT in four unique conformations and use these structures to classify the conformation of available PilT/VirB11-like family member structures. Single particle electron cryomicroscopy (cryoEM) structures of PilT reveal condition-dependent preferences for C2, C3, and C6 conformations. The physiologic importance of these conformations is validated by coevolution analysis and functional studies of point mutants, identifying a rare gain-of-function mutation that favours the C2 conformation. With these data, we propose a comprehensive model of PilT function with broad implications for PilT/VirB11-like family members.	+
-		+
-	~~Multiple conformations facilitate PilT function in the type IV pilus.,McCallum M, Benlekbir S, Nguyen S, Tammam S, Rubinstein JL, Burrows LL, Howell PL Nat Commun. 2019 Nov 15;10(1)~~:~~5198. doi: 10.1038~~/~~s41467-019-13070-z. PMID:31729381<ref>PMID:31729381<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6ojx" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Geomg~~]]	+	[[Category: Geobacter metallireducens GS-15]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Howell~~, P L~~]]	+	[[Category: Howell PL]]
-	[[Category: McCallum, M]]	+	[[Category: McCallum M]]
-	~~[[Category: Atpase]]~~	+
-	~~[[Category: Motor]]~~	+
-	~~[[Category: Motor protein]]~~	+
-	~~[[Category: T4p]]~~	+
-	~~[[Category: Type iv pilus~~]]	+

OCA at 09:07, 27 November 2019

OCA — Wed, 27 Nov 2019 09:07:29 GMT

←Older revision		Revision as of 09:07, 27 November 2019
Line 3:		Line 3:
	<StructureSection load='6ojx' size='340' side='right'caption='[[6ojx]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='6ojx' size='340' side='right'caption='[[6ojx]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ojx]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OJX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ojx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Geomg Geomg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OJX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pilT-4, Gmet_1394 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269799 GEOMG])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ojx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ojx OCA], [http://pdbe.org/6ojx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ojx RCSB], [http://www.ebi.ac.uk/pdbsum/6ojx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ojx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ojx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ojx OCA], [http://pdbe.org/6ojx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ojx RCSB], [http://www.ebi.ac.uk/pdbsum/6ojx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ojx ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type IV pilus-like systems are protein complexes that polymerize pilin fibres. They are critical for virulence in many bacterial pathogens. Pilin polymerization and depolymerization are powered by motor ATPases of the PilT/VirB11-like family. This family is thought to operate with C2 symmetry; however, most of these ATPases crystallize with either C3 or C6 symmetric conformations. The relevance of these conformations is unclear. Here, we determine the X-ray structures of PilT in four unique conformations and use these structures to classify the conformation of available PilT/VirB11-like family member structures. Single particle electron cryomicroscopy (cryoEM) structures of PilT reveal condition-dependent preferences for C2, C3, and C6 conformations. The physiologic importance of these conformations is validated by coevolution analysis and functional studies of point mutants, identifying a rare gain-of-function mutation that favours the C2 conformation. With these data, we propose a comprehensive model of PilT function with broad implications for PilT/VirB11-like family members.
		+
		+	Multiple conformations facilitate PilT function in the type IV pilus.,McCallum M, Benlekbir S, Nguyen S, Tammam S, Rubinstein JL, Burrows LL, Howell PL Nat Commun. 2019 Nov 15;10(1):5198. doi: 10.1038/s41467-019-13070-z. PMID:31729381<ref>PMID:31729381</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ojx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Geomg]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Howell, P L]]		[[Category: Howell, P L]]

OCA at 15:28, 20 November 2019

OCA — Wed, 20 Nov 2019 15:28:04 GMT

←Older revision		Revision as of 15:28, 20 November 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6ojx ~~is ON HOLD until Paper Publication~~	+	==PilT4 from Geobacter metallireducens bound to ATP==
-		+	<StructureSection load='6ojx' size='340' side='right'caption='[[6ojx]], [[Resolution\|resolution]] 1.89Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6ojx]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OJX FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ojx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ojx OCA], [http://pdbe.org/6ojx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ojx RCSB], [http://www.ebi.ac.uk/pdbsum/6ojx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ojx ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Howell, P L]]
		+	[[Category: McCallum, M]]
		+	[[Category: Atpase]]
		+	[[Category: Motor]]
		+	[[Category: Motor protein]]
		+	[[Category: T4p]]
		+	[[Category: Type iv pilus]]

OCA at 07:50, 10 May 2019

OCA — Fri, 10 May 2019 07:50:27 GMT

←Older revision		Revision as of 07:50, 10 May 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ojx is ON HOLD	+	The entry 6ojx is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6ojx" [edit=sysop:move=sysop]

OCA — Wed, 24 Apr 2019 04:56:59 GMT

Protected "6ojx" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:56, 24 April 2019

OCA: New page: '''Unreleased structure''' The entry 6ojx is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 24 Apr 2019 04:56:57 GMT

New page: '''Unreleased structure''' The entry 6ojx is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ojx is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]