6oky - Revision history

OCA at 10:57, 14 June 2023

OCA — Wed, 14 Jun 2023 10:57:10 GMT

←Older revision		Revision as of 10:57, 14 June 2023
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	==Solution structure of truncated peptide from PAMap53==		==Solution structure of truncated peptide from PAMap53==
-	<StructureSection load='6oky' size='340' side='right'caption='[[6oky~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='6oky' size='340' side='right'caption='[[6oky]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6oky]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OKY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6OKY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6oky]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OKY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6oky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oky OCA], [~~http~~://pdbe.org/6oky PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6oky RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6oky PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6oky ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oky OCA], [https://pdbe.org/6oky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oky RCSB], [https://www.ebi.ac.uk/pdbsum/6oky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oky ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PAM_STRPY PAM_STRPY]] Binds to human plasminogen (and plasmin) via its kringle repeats. Also binds to albumin, immunoglobulin G and fibrinogen. Could provide the bacteria with a mechanism for invasion, as streptococcal-bound plasmin could permit tissue penetration.	+	[https://www.uniprot.org/uniprot/PAM_STRPY PAM_STRPY] Binds to human plasminogen (and plasmin) via its kringle repeats. Also binds to albumin, immunoglobulin G and fibrinogen. Could provide the bacteria with a mechanism for invasion, as streptococcal-bound plasmin could permit tissue penetration.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6oky" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6oky" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tryptophan synthase 3D structures\|Tryptophan synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Castellino, F J~~]]	+	[[Category: Streptococcus pyogenes]]
-	[[Category: ~~Yuan, Y~~]]	+	[[Category: Castellino FJ]]
-	[[Category: ~~Plasminogen binding peptide]]~~	+	[[Category: Yuan Y]]
-	~~[[Category: Protein binding~~]]	+

OCA at 09:18, 26 February 2020

OCA — Wed, 26 Feb 2020 09:18:21 GMT

←Older revision		Revision as of 09:18, 26 February 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6oky is ~~ON HOLD until Paper~~ Publication	+	==Solution structure of truncated peptide from PAMap53==
		+	<StructureSection load='6oky' size='340' side='right'caption='[[6oky]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6oky]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OKY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OKY FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6oky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oky OCA], [http://pdbe.org/6oky PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oky RCSB], [http://www.ebi.ac.uk/pdbsum/6oky PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oky ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PAM_STRPY PAM_STRPY]] Binds to human plasminogen (and plasmin) via its kringle repeats. Also binds to albumin, immunoglobulin G and fibrinogen. Could provide the bacteria with a mechanism for invasion, as streptococcal-bound plasmin could permit tissue penetration.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	VEK50 is a truncated peptide from a Streptococcal pyogenes surface human plasminogen (hPg) binding M-protein (PAM). VEK50 contains the full A-domain of PAM, which is responsible for its low nanomolar binding to hPg. The interaction of VEK50 with kringle 2, the PAM-binding domain in hPg (K2hPg), has been studied by high-resolution NMR spectroscopy. The data show that each VEK50 monomer in solution contains two tight binding sites for K2hPg, one each in the a1- (RH1; R(17)H(18)) and a2- (RH2; R(30)H(31)) repeats within the A-domain of VEK50. Two mutant forms of VEK50, viz., VEK50[RH1/AA] (VEK50(DeltaRH1)) and VEK50[RH2/AA] (VEK50(DeltaRH2)), were designed by replacing each RH with AA, thus eliminating one of the K2hPg binding sites within VEK50, and allowing separate study of each binding site. Using (13)C- and (15)N-labeled peptides, NMR-derived solution structures of VEK50 in its complex with K2hPg were solved. We conclude that the A-domain of PAM can accommodate two molecules of K2hPg docked within a short distance of each other, and the strength of the binding is slightly different for each site. The solution structure of the VEK50/K2hPg, complex, which is a reductionist model of the PAM/hPg complex, provides insights for the binding mechanism of PAM to a host protein, a process that is critical to S. pyogenes virulence.

-	~~Authors: Yuan~~, Y., Castellino, F.J.	+	Solution structural model of the complex of the binding regions of human plasminogen with its M-protein receptor from Streptococcus pyogenes.,Yuan Y, Ayinuola YA, Singh D, Ayinuola O, Mayfield JA, Quek A, Whisstock JC, Law RHP, Lee SW, Ploplis VA, Castellino FJ J Struct Biol. 2019 Jul 10. pii: S1047-8477(19)30154-6. doi:, 10.1016/j.jsb.2019.07.005. PMID:31301349<ref>PMID:31301349</ref>

-	~~Description: Solution structure~~ of ~~truncated peptide from PAMap53~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6oky" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Castellino, F J]]
	[[Category: Yuan, Y]]		[[Category: Yuan, Y]]
-	[[Category: ~~Castellino, F.J~~]]	+	[[Category: Plasminogen binding peptide]]
		+	[[Category: Protein binding]]

OCA at 07:51, 10 May 2019

OCA — Fri, 10 May 2019 07:51:09 GMT

←Older revision		Revision as of 07:51, 10 May 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6oky is ON HOLD	+	The entry 6oky is ON HOLD until Paper Publication

	Authors: Yuan, Y., Castellino, F.J.		Authors: Yuan, Y., Castellino, F.J.

OCA: Protected "6oky" [edit=sysop:move=sysop]

OCA — Wed, 24 Apr 2019 05:00:49 GMT

Protected "6oky" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:00, 24 April 2019

OCA: New page: '''Unreleased structure''' The entry 6oky is ON HOLD Authors: Yuan, Y., Castellino, F.J. Description: Solution structure of truncated peptide from PAMap53 [[Category: Unreleased Struct...

OCA — Wed, 24 Apr 2019 05:00:47 GMT

New page: '''Unreleased structure''' The entry 6oky is ON HOLD Authors: Yuan, Y., Castellino, F.J. Description: Solution structure of truncated peptide from PAMap53 [[Category: Unreleased Struct...

New page

'''Unreleased structure'''

The entry 6oky is ON HOLD

Authors: Yuan, Y., Castellino, F.J.

Description: Solution structure of truncated peptide from PAMap53
[[Category: Unreleased Structures]]
[[Category: Yuan, Y]]
[[Category: Castellino, F.J]]