6olt - Revision history

OCA at 07:12, 11 October 2023

OCA — Wed, 11 Oct 2023 07:12:57 GMT

←Older revision		Revision as of 07:12, 11 October 2023
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	==Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabF, and C12-crypto Acyl Carrier Protein, AcpP==		==Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabF, and C12-crypto Acyl Carrier Protein, AcpP==
-	<StructureSection load='6olt' size='340' side='right'caption='[[6olt]]' scene=''>	+	<StructureSection load='6olt' size='340' side='right'caption='[[6olt]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OLT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6OLT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6olt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OLT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OLT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6olt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6olt OCA], [~~http~~://pdbe.org/6olt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6olt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6olt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6olt ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRJ:N-[2-(dodecanoylamino)ethyl]-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide'>MRJ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6olt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6olt OCA], [https://pdbe.org/6olt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6olt RCSB], [https://www.ebi.ac.uk/pdbsum/6olt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6olt ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FABF_ECOLI FABF_ECOLI] Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Has a preference for short chain acid substrates and may function to supply the octanoic substrates for lipoic acid biosynthesis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Carbon-carbon bond forming reactions are essential transformations in natural product biosynthesis. During de novo fatty acid and polyketide biosynthesis, beta-ketoacyl-acyl carrier protein (ACP) synthases (KS), catalyze this process via a decarboxylative Claisen-like condensation reaction. KSs must recognize multiple chemically distinct ACPs and choreograph a ping-pong mechanism, often in an iterative fashion. Here, we report crystal structures of substrate mimetic bearing ACPs in complex with the elongating KSs from Escherichia coli, FabF and FabB, in order to better understand the stereochemical features governing substrate discrimination by KSs. Complemented by molecular dynamics (MD) simulations and mutagenesis studies, these structures reveal conformational states accessed during KS catalysis. These data taken together support a gating mechanism that regulates acyl-ACP binding and substrate delivery to the KS active site. Two active site loops undergo large conformational excursions during this dynamic gating mechanism and are likely evolutionarily conserved features in elongating KSs.
		+
		+	Gating mechanism of elongating beta-ketoacyl-ACP synthases.,Mindrebo JT, Patel A, Kim WE, Davis TD, Chen A, Bartholow TG, La Clair JJ, McCammon JA, Noel JP, Burkart MD Nat Commun. 2020 Apr 7;11(1):1727. doi: 10.1038/s41467-020-15455-x. PMID:32265440<ref>PMID:32265440</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6olt" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acyl carrier protein 3D structures\|Acyl carrier protein 3D structures]]
		+	*[[Acyl carrier protein synthase 3D structures\|Acyl carrier protein synthase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bartholow TG]]		[[Category: Bartholow TG]]

OCA at 05:41, 22 April 2020

OCA — Wed, 22 Apr 2020 05:41:25 GMT

←Older revision		Revision as of 05:41, 22 April 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6olt ~~is ON HOLD until Paper Publication~~	+	==Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabF, and C12-crypto Acyl Carrier Protein, AcpP==
-		+	<StructureSection load='6olt' size='340' side='right'caption='[[6olt]]' scene=''>
-	~~Authors~~: ~~Mindrebo, J~~.T.~~, Kim, W~~.E.~~, Bartholow, T~~.G.~~, Chen, A~~., ~~Davis, T~~.D., ~~La Clair~~, J., ~~Burkart, M~~.D., ~~Noel, J~~.P.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OLT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OLT FirstGlance]. <br>
-	~~Description: Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabF, and C12-crypto Acyl Carrier Protein, AcpP~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6olt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6olt OCA], [http://pdbe.org/6olt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6olt RCSB], [http://www.ebi.ac.uk/pdbsum/6olt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6olt ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Chen, A~~]]	+	__TOC__
-	[[Category: ~~Bartholow, T.G~~]]	+	</StructureSection>
-	[[Category: ~~Noel, J.P~~]]	+	[[Category: Large Structures]]
-	[[Category: Davis~~, T.D~~]]	+	[[Category: Bartholow TG]]
-	[[Category: ~~Burkart, M.D~~]]	+	[[Category: Burkart MD]]
-	[[Category: ~~Mindrebo,~~ J.T]]	+	[[Category: Chen A]]
-	[[Category: ~~Kim, W.E~~]]	+	[[Category: Davis TD]]
-	[[Category: ~~La Clair, J~~]]	+	[[Category: Kim WE]]
		+	[[Category: La Clair J]]
		+	[[Category: Mindrebo JT]]
		+	[[Category: Noel JP]]

OCA at 07:08, 1 May 2019

OCA — Wed, 01 May 2019 07:08:15 GMT

←Older revision		Revision as of 07:08, 1 May 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6olt is ON HOLD	+	The entry 6olt is ON HOLD until Paper Publication

	Authors: Mindrebo, J.T., Kim, W.E., Bartholow, T.G., Chen, A., Davis, T.D., La Clair, J., Burkart, M.D., Noel, J.P.		Authors: Mindrebo, J.T., Kim, W.E., Bartholow, T.G., Chen, A., Davis, T.D., La Clair, J., Burkart, M.D., Noel, J.P.

OCA: Protected "6olt" [edit=sysop:move=sysop]

OCA — Wed, 24 Apr 2019 05:03:44 GMT

Protected "6olt" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:03, 24 April 2019

OCA: New page: '''Unreleased structure''' The entry 6olt is ON HOLD Authors: Mindrebo, J.T., Kim, W.E., Bartholow, T.G., Chen, A., Davis, T.D., La Clair, J., Burkart, M.D., Noel, J.P. Description: Cr...

OCA — Wed, 24 Apr 2019 05:03:42 GMT

New page: '''Unreleased structure''' The entry 6olt is ON HOLD Authors: Mindrebo, J.T., Kim, W.E., Bartholow, T.G., Chen, A., Davis, T.D., La Clair, J., Burkart, M.D., Noel, J.P. Description: Cr...

New page

'''Unreleased structure'''

The entry 6olt is ON HOLD

Authors: Mindrebo, J.T., Kim, W.E., Bartholow, T.G., Chen, A., Davis, T.D., La Clair, J., Burkart, M.D., Noel, J.P.

Description: Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabF, and C12-crypto Acyl Carrier Protein, AcpP
[[Category: Unreleased Structures]]
[[Category: Chen, A]]
[[Category: Bartholow, T.G]]
[[Category: Noel, J.P]]
[[Category: Davis, T.D]]
[[Category: Burkart, M.D]]
[[Category: Mindrebo, J.T]]
[[Category: Kim, W.E]]
[[Category: La Clair, J]]