6ovu - Revision history

OCA at 06:02, 20 May 2020

OCA — Wed, 20 May 2020 06:02:01 GMT

←Older revision		Revision as of 06:02, 20 May 2020
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	==Coiled-coil Trimer with Glu:3,4-difluorophenylalanine:Lys Triad==		==Coiled-coil Trimer with Glu:3,4-difluorophenylalanine:Lys Triad==
-	<StructureSection load='6ovu' size='340' side='right'caption='[[6ovu]]' scene=''>	+	<StructureSection load='6ovu' size='340' side='right'caption='[[6ovu]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OVU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OVU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ovu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OVU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OVU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ovu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ovu OCA], [http://pdbe.org/6ovu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ovu RCSB], [http://www.ebi.ac.uk/pdbsum/6ovu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ovu ProSAT]</span></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=F2F:3,4-DIFLUORO-L-PHENYLALANINE'>F2F</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ovu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ovu OCA], [http://pdbe.org/6ovu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ovu RCSB], [http://www.ebi.ac.uk/pdbsum/6ovu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ovu ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Here we show that a solvent-exposed f-position (i.e., residue 14) within a well-characterized trimeric helix bundle can facilitate a stabilizing long-range synergistic interaction involving b-position Glu10 (i.e., i - 4 relative to residue 14) and c-position Lys18 (i.e., i + 4), depending the identity of residue 14. The extent of stabilization associated with the Glu10-Lys18 pair depends primarily on the presence of a side-chain hydrogen-bond donor at residue 14; the nonpolar or hydrophobic character of residue 14 plays a smaller but still significant role. Crystal structures and molecular dynamics simulations indicate that Glu10 and Lys18 do not interact directly with each other but suggest the possibility that the proximity of residue 14 with Lys18 allows Glu10 to interact favorably with nearby Lys7. Subsequent thermodynamic experiments confirm the important role of Lys7 in the large synergistic stabilization associated with the Glu10-Lys18 pair. Our results highlight the exquisite complexity and surprising long-range synergistic interactions among b-, c-, and f-position residues within helix bundles, suggesting new possibilities for engineering hyperstable helix bundles and emphasizing the need to consider carefully the impact of substitutions at these positions for application-specific purposes.
		+
		+	Context-Dependent Stabilizing Interactions among Solvent-Exposed Residues along the Surface of a Trimeric Helix Bundle.,Stern KL, Smith MS, Billings WM, Loftus TJ, Conover BM, Della Corte D, Price JL Biochemistry. 2020 Apr 20. doi: 10.1021/acs.biochem.0c00045. PMID:32270676<ref>PMID:32270676</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ovu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Billings WM]]	+	[[Category: Billings, W M]]
-	[[Category: Price JL]]	+	[[Category: Price, J L]]
-	[[Category: Smith MS]]	+	[[Category: Smith, M S]]
-	[[Category: Stern KL]]	+	[[Category: Stern, K L]]
		+	[[Category: De novo protein]]
		+	[[Category: Helix]]
		+	[[Category: Trimer]]

OCA at 06:42, 29 April 2020

OCA — Wed, 29 Apr 2020 06:42:21 GMT

←Older revision		Revision as of 06:42, 29 April 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6ovu ~~is ON HOLD until Paper Publication~~	+	==Coiled-coil Trimer with Glu:3,4-difluorophenylalanine:Lys Triad==
-		+	<StructureSection load='6ovu' size='340' side='right'caption='[[6ovu]]' scene=''>
-	~~Authors~~: ~~Smith~~, M.S., ~~Stern~~, K.L., ~~Billings, W~~.M., ~~Price, J~~.L.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OVU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OVU FirstGlance]. <br>
-	~~Description: Coiled-coil Trimer with Glu:3,4-difluorophenylalanine:Lys Triad~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ovu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ovu OCA], [http://pdbe.org/6ovu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ovu RCSB], [http://www.ebi.ac.uk/pdbsum/6ovu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ovu ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Price, J.L~~]]	+	__TOC__
-	[[Category: ~~Smith, M.S~~]]	+	</StructureSection>
-	[[Category: ~~Stern, K.L~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Billings, W.M~~]]	+	[[Category: Billings WM]]
		+	[[Category: Price JL]]
		+	[[Category: Smith MS]]
		+	[[Category: Stern KL]]

OCA at 08:53, 17 July 2019

OCA — Wed, 17 Jul 2019 08:53:09 GMT

←Older revision		Revision as of 08:53, 17 July 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ovu is ON HOLD	+	The entry 6ovu is ON HOLD until Paper Publication

	Authors: Smith, M.S., Stern, K.L., Billings, W.M., Price, J.L.		Authors: Smith, M.S., Stern, K.L., Billings, W.M., Price, J.L.

OCA: Protected "6ovu" [edit=sysop:move=sysop]

OCA — Tue, 21 May 2019 05:53:28 GMT

Protected "6ovu" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:53, 21 May 2019

OCA: New page: '''Unreleased structure''' The entry 6ovu is ON HOLD Authors: Smith, M.S., Stern, K.L., Billings, W.M., Price, J.L. Description: Coiled-coil Trimer with Glu:3,4-difluorophenylalanine:L...

OCA — Tue, 21 May 2019 05:53:26 GMT

New page: '''Unreleased structure''' The entry 6ovu is ON HOLD Authors: Smith, M.S., Stern, K.L., Billings, W.M., Price, J.L. Description: Coiled-coil Trimer with Glu:3,4-difluorophenylalanine:L...

New page

'''Unreleased structure'''

The entry 6ovu is ON HOLD

Authors: Smith, M.S., Stern, K.L., Billings, W.M., Price, J.L.

Description: Coiled-coil Trimer with Glu:3,4-difluorophenylalanine:Lys Triad
[[Category: Unreleased Structures]]
[[Category: Price, J.L]]
[[Category: Smith, M.S]]
[[Category: Stern, K.L]]
[[Category: Billings, W.M]]