6oyv - Revision history

OCA at 07:19, 11 October 2023

OCA — Wed, 11 Oct 2023 07:19:51 GMT

←Older revision		Revision as of 07:19, 11 October 2023
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	<StructureSection load='6oyv' size='340' side='right'caption='[[6oyv]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='6oyv' size='340' side='right'caption='[[6oyv]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6oyv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OYV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6OYV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6oyv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OYV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OYV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EST:ESTRADIOL'>EST</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.101Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6oyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oyv OCA], [~~http~~://pdbe.org/6oyv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6oyv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6oyv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6oyv ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EST:ESTRADIOL'>EST</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oyv OCA], [https://pdbe.org/6oyv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oyv RCSB], [https://www.ebi.ac.uk/pdbsum/6oyv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oyv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 6oyv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6oyv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 21:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Synthetic construct ~~sequences~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Bart~~, A G~~]]	+	[[Category: Bart AG]]
-	[[Category: Scott~~, E E]]~~	+	[[Category: Scott EE]]
-	~~[[Category: Ancestral reconstruction]]~~	+
-	~~[[Category: Cyp1b1]]~~	+
-	~~[[Category: Mammalian]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 06:30, 6 May 2020

OCA — Wed, 06 May 2020 06:30:59 GMT

←Older revision		Revision as of 06:30, 6 May 2020
Line 3:		Line 3:
	<StructureSection load='6oyv' size='340' side='right'caption='[[6oyv]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='6oyv' size='340' side='right'caption='[[6oyv]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6oyv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OYV OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6OYV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6oyv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OYV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OYV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EST:ESTRADIOL'>EST</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EST:ESTRADIOL'>EST</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6oyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oyv OCA], [http://pdbe.org/6oyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oyv RCSB], [http://www.ebi.ac.uk/pdbsum/6oyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oyv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6oyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oyv OCA], [http://pdbe.org/6oyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oyv RCSB], [http://www.ebi.ac.uk/pdbsum/6oyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oyv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:36, 27 March 2020

OCA — Fri, 27 Mar 2020 10:36:10 GMT

←Older revision		Revision as of 10:36, 27 March 2020
Line 3:		Line 3:
	<StructureSection load='6oyv' size='340' side='right'caption='[[6oyv]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='6oyv' size='340' side='right'caption='[[6oyv]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6oyv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OYV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6oyv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OYV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EST:ESTRADIOL'>EST</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EST:ESTRADIOL'>EST</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6oyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oyv OCA], [http://pdbe.org/6oyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oyv RCSB], [http://www.ebi.ac.uk/pdbsum/6oyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oyv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6oyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oyv OCA], [http://pdbe.org/6oyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oyv RCSB], [http://www.ebi.ac.uk/pdbsum/6oyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oyv ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mammalian cytochrome P450 enzymes often metabolize many pharmaceuticals and other xenobiotics, a feature that is valuable in a biotechnology setting. However, extant P450 enzymes are typically relatively unstable with T50 values ~30-40 degrees C. Reconstructed ancestral cytochrome P450 enzymes tend to have variable substrate selectivity compared to related extant forms, but also have higher thermostability and therefore may be excellent tools for commercial biosynthesis of important intermediates, final drug molecules, or drug metabolites. The mammalian ancestor of the cytochrome P450 1B subfamily was herein characterized structurally and functionally, revealing differences from the extant human CYP1B1 in ligand binding, metabolism, and potential molecular contributors to its thermostability. While extant human CYP1B1 has one molecule of alpha-naphthoflavone in a closed active site, we observed that subtle amino acid substitutions outside the active site in the ancestor CYP1B enzyme yielded an open active site with four ligand copies. A structure of the ancestor with 17beta-estradiol revealed only one molecule in the active site, which still had the same open conformation. Detailed comparisons between the extant and ancestor forms revealed increases in electrostatic and aromatic interactions between distinct secondary structure elements in the ancestral forms that may contribute to their thermostability. To the best of our knowledge, this represents the first structural evaluation of a reconstructed ancestral cytochrome P450, revealing key features that appear to contribute to their thermostability.
		+
		+	Structure of an ancestral mammalian family 1B1 cytochrome P450 with increased thermostability.,Bart AG, Harris KL, Gillam EMJ, Scott EE J Biol Chem. 2020 Mar 10. pii: RA119.010727. doi: 10.1074/jbc.RA119.010727. PMID:32156703<ref>PMID:32156703</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6oyv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Synthetic construct sequences]]
	[[Category: Bart, A G]]		[[Category: Bart, A G]]
	[[Category: Scott, E E]]		[[Category: Scott, E E]]

OCA at 09:43, 18 March 2020

OCA — Wed, 18 Mar 2020 09:43:27 GMT

←Older revision		Revision as of 09:43, 18 March 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6oyv ~~is ON HOLD until Paper Publication~~	+	==Structure of an ancestral-reconstructed cytochrome P450 1B1 with estradiol==
-		+	<StructureSection load='6oyv' size='340' side='right'caption='[[6oyv]], [[Resolution\|resolution]] 3.10Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6oyv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OYV FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EST:ESTRADIOL'>EST</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6oyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oyv OCA], [http://pdbe.org/6oyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oyv RCSB], [http://www.ebi.ac.uk/pdbsum/6oyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oyv ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bart, A G]]
		+	[[Category: Scott, E E]]
		+	[[Category: Ancestral reconstruction]]
		+	[[Category: Cyp1b1]]
		+	[[Category: Mammalian]]
		+	[[Category: Oxidoreductase]]

OCA at 04:31, 29 May 2019

OCA — Wed, 29 May 2019 04:31:58 GMT

←Older revision		Revision as of 04:31, 29 May 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6oyv is ON HOLD	+	The entry 6oyv is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6oyv" [edit=sysop:move=sysop]

OCA — Wed, 22 May 2019 04:07:37 GMT

Protected "6oyv" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:07, 22 May 2019

OCA: New page: '''Unreleased structure''' The entry 6oyv is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 May 2019 04:07:35 GMT

New page: '''Unreleased structure''' The entry 6oyv is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6oyv is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]