6p9i - Revision history

OCA at 07:25, 11 October 2023

OCA — Wed, 11 Oct 2023 07:25:35 GMT

←Older revision		Revision as of 07:25, 11 October 2023
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	<StructureSection load='6p9i' size='340' side='right'caption='[[6p9i]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6p9i' size='340' side='right'caption='[[6p9i]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6p9i]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P9I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6P9I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6p9i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6P9I FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6p9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p9i OCA], [~~http~~://pdbe.org/6p9i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6p9i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6p9i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6p9i ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6p9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p9i OCA], [https://pdbe.org/6p9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6p9i RCSB], [https://www.ebi.ac.uk/pdbsum/6p9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6p9i ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Crowe~~, J E~~]]	+	[[Category: Crowe JE]]
-	[[Category: Dong, J]]	+	[[Category: Dong J]]
-	~~[[Category: Fab]]~~	+
-	~~[[Category: Human antibody]]~~	+
-	~~[[Category: Immune system~~]]	+

OCA at 09:01, 6 January 2021

OCA — Wed, 06 Jan 2021 09:01:24 GMT

←Older revision		Revision as of 09:01, 6 January 2021
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	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6p9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p9i OCA], [http://pdbe.org/6p9i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p9i RCSB], [http://www.ebi.ac.uk/pdbsum/6p9i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p9i ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6p9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p9i OCA], [http://pdbe.org/6p9i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p9i RCSB], [http://www.ebi.ac.uk/pdbsum/6p9i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p9i ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Staphylococcus aureus is an important human pathogen that infects nearly every human tissue. Like most organisms, the acquisition of nutrient iron is necessary for its survival. One route by which it obtains this metal is through the iron-regulated surface determinant (Isd) system that scavenges iron from the hemoglobin of the host. We show that the heavy chain variable region IGHV1-69 gene commonly encodes human monoclonal antibodies (mAbs) targeting IsdB-NEAT2. Remarkably, these antibodies bind to multiple antigenic sites. One class of IGHV1-69-encoded mAbs blocks S. aureus heme acquisition by binding to the heme-binding site of NEAT2, while two additional classes reduce the bacterial burden in vivo by an alternative Fc receptor-mediated mechanism. We further identified clonal lineages of IGHV1-69-encoded mAbs using donor samples, showing that each lineage diversifies during infection by somatic hypermutation. These studies reveal that IGHV1-69-encoded antibodies contribute to a protective immune response, furthering our understanding of the correlates of protection against S. aureus infection.IMPORTANCE The human pathogen Staphylococcus aureus causes a wide range of infections, including skin abscesses and sepsis. There is currently no licensed vaccine to prevent S. aureus infection, and its treatment has become increasingly difficult due to antibiotic resistance. One potential way to inhibit S. aureus pathogenesis is to prevent iron acquisition. The iron-regulated surface determinant (Isd) system has evolved in S. aureus to acquire hemoglobin from the human host as a source of heme-iron. In this study, we investigated the molecular and structural basis for antibody-mediated correlates against a member of the Isd system, IsdB. The association of immunoglobulin heavy chain variable region IGHV1-69 gene-encoded human monoclonal antibodies with the response against S. aureus IsdB is described using structural and functional studies to define the importance of this antibody class. We also determine that somatic hypermutation in the development of these antibodies hinders rather than fine-tunes the immune response to IsdB.
		+
		+	Human VH1-69 Gene-Encoded Human Monoclonal Antibodies against Staphylococcus aureus IsdB Use at Least Three Distinct Modes of Binding To Inhibit Bacterial Growth and Pathogenesis.,Bennett MR, Dong J, Bombardi RG, Soto C, Parrington HM, Nargi RS, Schoeder CT, Nagel MB, Schey KL, Meiler J, Skaar EP, Crowe JE Jr mBio. 2019 Oct 22;10(5). pii: mBio.02473-19. doi: 10.1128/mBio.02473-19. PMID:31641091<ref>PMID:31641091</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6p9i" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:54, 30 December 2020

OCA — Wed, 30 Dec 2020 06:54:58 GMT

←Older revision		Revision as of 06:54, 30 December 2020
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	==crystal structure of human anti staphylococcus aureus antibody STAU-399 Fab==		==crystal structure of human anti staphylococcus aureus antibody STAU-399 Fab==
-	<StructureSection load='6p9i' size='340' side='right'caption='[[6p9i]]' scene=''>	+	<StructureSection load='6p9i' size='340' side='right'caption='[[6p9i]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P9I OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6P9I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6p9i]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P9I OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6P9I FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6p9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p9i OCA], [http://pdbe.org/6p9i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p9i RCSB], [http://www.ebi.ac.uk/pdbsum/6p9i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p9i ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6p9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p9i OCA], [http://pdbe.org/6p9i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p9i RCSB], [http://www.ebi.ac.uk/pdbsum/6p9i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p9i ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Crowe JE]]	+	[[Category: Crowe, J E]]
-	[[Category: Dong J]]	+	[[Category: Dong, J]]
		+	[[Category: Fab]]
		+	[[Category: Human antibody]]
		+	[[Category: Immune system]]

OCA at 10:09, 17 June 2020

OCA — Wed, 17 Jun 2020 10:09:20 GMT

←Older revision		Revision as of 10:09, 17 June 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6p9i ~~is ON HOLD until Paper Publication~~	+	==crystal structure of human anti staphylococcus aureus antibody STAU-399 Fab==
-		+	<StructureSection load='6p9i' size='340' side='right'caption='[[6p9i]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P9I OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6P9I FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6p9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p9i OCA], [http://pdbe.org/6p9i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p9i RCSB], [http://www.ebi.ac.uk/pdbsum/6p9i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p9i ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Crowe JE]]
		+	[[Category: Dong J]]

OCA: Protected "6p9i" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2019 04:20:48 GMT

Protected "6p9i" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:20, 19 June 2019

OCA: New page: '''Unreleased structure''' The entry 6p9i is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 19 Jun 2019 04:20:46 GMT

New page: '''Unreleased structure''' The entry 6p9i is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6p9i is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]