6pao - Revision history

OCA at 07:26, 11 October 2023

OCA — Wed, 11 Oct 2023 07:26:12 GMT

←Older revision		Revision as of 07:26, 11 October 2023
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	<StructureSection load='6pao' size='340' side='right'caption='[[6pao]], [[Resolution\|resolution]] 3.65Å' scene=''>		<StructureSection load='6pao' size='340' side='right'caption='[[6pao]], [[Resolution\|resolution]] 3.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pao]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Novad Novad~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PAO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6PAO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pao]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Novosphingobium_aromaticivorans_DSM_12444 Novosphingobium aromaticivorans DSM 12444]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PAO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ATP:ADENOSINE~~-~~5-TRIPHOSPHATE'>ATP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.65Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[6pam\|6pam]], [[6pan\|6pan]], [[6paq\|6paq]], [[6par\|6par]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~"><~~b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">atm1, Saro_2631 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=279238 NOVAD])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pao OCA], [https://pdbe.org/6pao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pao RCSB], [https://www.ebi.ac.uk/pdbsum/6pao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pao ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6pao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pao OCA], [~~http~~://pdbe.org/6pao PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6pao RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6pao PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6pao ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ATM1_NOVAD ATM1_NOVAD]] Mediates the ATP-dependent export of glutathione-conjugated substrates, such as heavy metal-glutathione conjugates. ATP hydrolysis is stimulated by glutathione binding. Protects cells against toxic heavy metal ions, such as silver and mercury ions. May also mediate the transport of glutathione-conjugated aromatic hydrocarbons, such as dinitrobenzene.<ref>PMID:24604198</ref>	+	[https://www.uniprot.org/uniprot/ATM1_NOVAD ATM1_NOVAD] Mediates the ATP-dependent export of glutathione-conjugated substrates, such as heavy metal-glutathione conjugates. ATP hydrolysis is stimulated by glutathione binding. Protects cells against toxic heavy metal ions, such as silver and mercury ions. May also mediate the transport of glutathione-conjugated aromatic hydrocarbons, such as dinitrobenzene.<ref>PMID:24604198</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Novad~~]]	+	[[Category: Novosphingobium aromaticivorans DSM 12444]]
-	[[Category: Fan, C]]	+	[[Category: Fan C]]
-	[[Category: Kaiser~~, J T~~]]	+	[[Category: Kaiser JT]]
-	[[Category: Rees~~, D C]]~~	+	[[Category: Rees DC]]
-	~~[[Category: Abc exporter]]~~	+
-	~~[[Category: Abc transporter]]~~	+
-	~~[[Category: Atpase]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 06:32, 19 August 2020

OCA — Wed, 19 Aug 2020 06:32:23 GMT

←Older revision		Revision as of 06:32, 19 August 2020
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	==Structure of a bacterial Atm1-family ABC exporter with ATP bound==		==Structure of a bacterial Atm1-family ABC exporter with ATP bound==
-	<StructureSection load='6pao' size='340' side='right'caption='[[6pao]]' scene=''>	+	<StructureSection load='6pao' size='340' side='right'caption='[[6pao]], [[Resolution\|resolution]] 3.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PAO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PAO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pao]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Novad Novad]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PAO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PAO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pao OCA], [http://pdbe.org/6pao PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pao RCSB], [http://www.ebi.ac.uk/pdbsum/6pao PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pao ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6pam\|6pam]], [[6pan\|6pan]], [[6paq\|6paq]], [[6par\|6par]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atm1, Saro_2631 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=279238 NOVAD])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pao OCA], [http://pdbe.org/6pao PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pao RCSB], [http://www.ebi.ac.uk/pdbsum/6pao PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pao ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ATM1_NOVAD ATM1_NOVAD]] Mediates the ATP-dependent export of glutathione-conjugated substrates, such as heavy metal-glutathione conjugates. ATP hydrolysis is stimulated by glutathione binding. Protects cells against toxic heavy metal ions, such as silver and mercury ions. May also mediate the transport of glutathione-conjugated aromatic hydrocarbons, such as dinitrobenzene.<ref>PMID:24604198</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ATP-binding cassette (ABC) transporter of mitochondria (Atm1) mediates iron homeostasis in eukaryotes, while the prokaryotic homolog from Novosphingobium aromaticivorans (NaAtm1) can export glutathione derivatives and confer protection against heavy-metal toxicity. To establish the structural framework underlying the NaAtm1 transport mechanism, we determined eight structures by X-ray crystallography and single-particle cryo-electron microscopy in distinct conformational states, stabilized by individual disulfide crosslinks and nucleotides. As NaAtm1 progresses through the transport cycle, conformational changes in transmembrane helix 6 (TM6) alter the glutathione-binding site and the associated substrate-binding cavity. Significantly, kinking of TM6 in the post-ATP hydrolysis state stabilized by MgADPVO4 eliminates this cavity, precluding uptake of glutathione derivatives. The presence of this cavity during the transition from the inward-facing to outward-facing conformational states, and its absence in the reverse direction, thereby provide an elegant and conceptually simple mechanism for enforcing the export directionality of transport by NaAtm1. One of the disulfide crosslinked NaAtm1 variants characterized in this work retains significant glutathione transport activity, suggesting that ATP hydrolysis and substrate transport by Atm1 may involve a limited set of conformational states with minimal separation of the nucleotide-binding domains in the inward-facing conformation.
		+
		+	A structural framework for unidirectional transport by a bacterial ABC exporter.,Fan C, Kaiser JT, Rees DC Proc Natl Acad Sci U S A. 2020 Aug 11;117(32):19228-19236. doi:, 10.1073/pnas.2006526117. Epub 2020 Jul 23. PMID:32703810<ref>PMID:32703810</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6pao" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fan C]]	+	[[Category: Novad]]
-	[[Category: Kaiser JT]]	+	[[Category: Fan, C]]
-	[[Category: Rees DC]]	+	[[Category: Kaiser, J T]]
		+	[[Category: Rees, D C]]
		+	[[Category: Abc exporter]]
		+	[[Category: Abc transporter]]
		+	[[Category: Atpase]]
		+	[[Category: Membrane protein]]
		+	[[Category: Transport protein]]

OCA at 06:18, 25 June 2020

OCA — Thu, 25 Jun 2020 06:18:20 GMT

←Older revision		Revision as of 06:18, 25 June 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6pao ~~is ON HOLD until Paper Publication~~	+	==Structure of a bacterial Atm1-family ABC exporter with ATP bound==
-		+	<StructureSection load='6pao' size='340' side='right'caption='[[6pao]]' scene=''>
-	~~Authors~~: ~~Fan~~, C., ~~Kaiser~~, J.T., ~~Rees~~, D.C.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PAO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PAO FirstGlance]. <br>
-	~~Description: Structure of a bacterial Atm1-family ABC exporter with ATP bound~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pao OCA], [http://pdbe.org/6pao PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pao RCSB], [http://www.ebi.ac.uk/pdbsum/6pao PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pao ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Kaiser, J.T~~]]	+	__TOC__
-	[[Category: ~~Rees, D.C~~]]	+	</StructureSection>
-	[[Category: ~~Fan, C~~]]	+	[[Category: Large Structures]]
		+	[[Category: Fan C]]
		+	[[Category: Kaiser JT]]
		+	[[Category: Rees DC]]

OCA at 13:12, 29 January 2020

OCA — Wed, 29 Jan 2020 13:12:06 GMT

←Older revision		Revision as of 13:12, 29 January 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pao is ON HOLD	+	The entry 6pao is ON HOLD until Paper Publication

	Authors: Fan, C., Kaiser, J.T., Rees, D.C.		Authors: Fan, C., Kaiser, J.T., Rees, D.C.

OCA: Protected "6pao" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2019 04:23:55 GMT

Protected "6pao" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:23, 19 June 2019

OCA: New page: '''Unreleased structure''' The entry 6pao is ON HOLD Authors: Fan, C., Kaiser, J.T., Rees, D.C. Description: Structure of a bacterial Atm1-family ABC exporter with ATP bound [[Category...

OCA — Wed, 19 Jun 2019 04:23:54 GMT

New page: '''Unreleased structure''' The entry 6pao is ON HOLD Authors: Fan, C., Kaiser, J.T., Rees, D.C. Description: Structure of a bacterial Atm1-family ABC exporter with ATP bound [[Category...

New page

'''Unreleased structure'''

The entry 6pao is ON HOLD

Authors: Fan, C., Kaiser, J.T., Rees, D.C.

Description: Structure of a bacterial Atm1-family ABC exporter with ATP bound
[[Category: Unreleased Structures]]
[[Category: Kaiser, J.T]]
[[Category: Rees, D.C]]
[[Category: Fan, C]]