OCA at 07:23, 11 March 2020

OCA — Wed, 11 Mar 2020 07:23:54 GMT

←Older revision		Revision as of 07:23, 11 March 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6pci is ~~ON HOLD until Paper~~ Publication	+	==EBOV GPdMuc (Makona) in complex with rEBOV-520 and rEBOV-548 Fabs==
		+	<StructureSection load='6pci' size='340' side='right'caption='[[6pci]], [[Resolution\|resolution]] 4.12Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6pci]] is a 18 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PCI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PCI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pci OCA], [http://pdbe.org/6pci PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pci RCSB], [http://www.ebi.ac.uk/pdbsum/6pci PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pci ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Structural principles underlying the composition of protective antiviral monoclonal antibody (mAb) cocktails are poorly defined. Here, we exploited antibody cooperativity to develop a therapeutic mAb cocktail against Ebola virus. We systematically analyzed the antibody repertoire in human survivors and identified a pair of potently neutralizing mAbs that cooperatively bound to the ebolavirus glycoprotein (GP). High-resolution structures revealed that in a two-antibody cocktail, molecular mimicry was a major feature of mAb-GP interactions. Broadly neutralizing mAb rEBOV-520 targeted a conserved epitope on the GP base region. mAb rEBOV-548 bound to a glycan cap epitope, possessed neutralizing and Fc-mediated effector function activities, and potentiated neutralization by rEBOV-520. Remodeling of the glycan cap structures by the cocktail enabled enhanced GP binding and virus neutralization. The cocktail demonstrated resistance to virus escape and protected non-human primates (NHPs) against Ebola virus disease. These data illuminate structural principles of antibody cooperativity with implications for development of antiviral immunotherapeutics.

-	~~Authors: Ward, A.B~~., Murin, C.D., ~~Alkutkar, T~~.	+	Analysis of a Therapeutic Antibody Cocktail Reveals Determinants for Cooperative and Broad Ebolavirus Neutralization.,Gilchuk P, Murin CD, Milligan JC, Cross RW, Mire CE, Ilinykh PA, Huang K, Kuzmina N, Altman PX, Hui S, Gunn BM, Bryan AL, Davidson E, Doranz BJ, Turner HL, Alkutkar T, Flinko R, Orlandi C, Carnahan R, Nargi R, Bombardi RG, Vodzak ME, Li S, Okoli A, Ibeawuchi M, Ohiaeri B, Lewis GK, Alter G, Bukreyev A, Saphire EO, Geisbert TW, Ward AB, Crowe JE Jr Immunity. 2020 Feb 18;52(2):388-403.e12. doi: 10.1016/j.immuni.2020.01.001. Epub , 2020 Feb 4. PMID:32023489<ref>PMID:32023489</ref>

-	~~Description: EBOV GPdMuc (Makona) in complex with rEBOV~~-~~520 and rEBOV~~-~~548 Fabs~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Ward, A.B]]~~	+	<div class="pdbe-citations 6pci" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Murin, C.D~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Alkutkar, T]]		[[Category: Alkutkar, T]]
		+	[[Category: Murin, C D]]
		+	[[Category: Ward, A B]]
		+	[[Category: Antibody]]
		+	[[Category: Ebola]]
		+	[[Category: Filovirus]]
		+	[[Category: Immune system]]
		+	[[Category: Synergy]]
		+	[[Category: Viral protein]]

OCA: Protected "6pci" [edit=sysop:move=sysop]

OCA — Wed, 26 Jun 2019 05:16:29 GMT

Protected "6pci" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:16, 26 June 2019

OCA: New page: '''Unreleased structure''' The entry 6pci is ON HOLD until Paper Publication Authors: Ward, A.B., Murin, C.D., Alkutkar, T. Description: EBOV GPdMuc (Makona) in complex with rEBOV-520 ...

OCA — Wed, 26 Jun 2019 05:16:28 GMT

New page: '''Unreleased structure''' The entry 6pci is ON HOLD until Paper Publication Authors: Ward, A.B., Murin, C.D., Alkutkar, T. Description: EBOV GPdMuc (Makona) in complex with rEBOV-520 ...

New page

'''Unreleased structure'''

The entry 6pci is ON HOLD until Paper Publication

Authors: Ward, A.B., Murin, C.D., Alkutkar, T.

Description: EBOV GPdMuc (Makona) in complex with rEBOV-520 and rEBOV-548 Fabs
[[Category: Unreleased Structures]]
[[Category: Ward, A.B]]
[[Category: Murin, C.D]]
[[Category: Alkutkar, T]]

6pci - Revision history

OCA at 07:23, 11 March 2020

OCA: Protected "6pci" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6pci is ON HOLD until Paper Publication Authors: Ward, A.B., Murin, C.D., Alkutkar, T. Description: EBOV GPdMuc (Makona) in complex with rEBOV-520 ...