6pcz - Revision history

OCA at 05:50, 13 May 2020

OCA — Wed, 13 May 2020 05:50:04 GMT

←Older revision		Revision as of 05:50, 13 May 2020
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	<StructureSection load='6pcz' size='340' side='right'caption='[[6pcz]], [[Resolution\|resolution]] 1.44Å' scene=''>		<StructureSection load='6pcz' size='340' side='right'caption='[[6pcz]], [[Resolution\|resolution]] 1.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pcz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PCZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6PCZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pcz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PCZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PCZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6pcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pcz OCA], [http://pdbe.org/6pcz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pcz RCSB], [http://www.ebi.ac.uk/pdbsum/6pcz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pcz ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bcsG, yhjU, b3538, JW3506 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pcz OCA], [http://pdbe.org/6pcz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pcz RCSB], [http://www.ebi.ac.uk/pdbsum/6pcz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pcz ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial biofilms are cellular communities that produce an adherent matrix. Exopolysaccharides are key structural components of this matrix and are required for the assembly and architecture of biofilms produced by a wide variety of microorganisms. The human bacterial pathogens Escherichia coli and Salmonella enterica produce a biofilm matrix composed primarily of the exopolysaccharide phosphoethanolamine (pEtN) cellulose. Once thought to be composed of only underivatized cellulose, the pEtN modification present in these matrices has been implicated in the overall architecture and integrity of the biofilm. However, an understanding of the mechanism underlying pEtN derivatization of the cellulose exopolysaccharide remains elusive. The bacterial cellulose synthase subunit G (BcsG) is a predicted inner membrane-localized metalloenzyme that has been proposed to catalyze the transfer of the pEtN group from membrane phospholipids to cellulose. Here we present evidence that the C-terminal domain of BcsG from E. coli (EcBcsG(DeltaN)) functions as a phosphoethanolamine transferase in vitro with substrate preference for cellulosic materials. Structural characterization of EcBcsG(DeltaN) revealed that it belongs to the alkaline phosphatase superfamily, contains a Zn(2+) ion at its active center, and is structurally similar to characterized enzymes that confer colistin resistance in Gram-negative bacteria. Informed by our structural studies, we present a functional complementation experiment in E. coli AR3110, indicating that the activity of the BcsG C-terminal domain is essential for integrity of the pellicular biofilm. Furthermore, our results established a similar but distinct active-site architecture and catalytic mechanism shared between BcsG and the colistin resistance enzymes.
		+
		+	The Escherichia coli cellulose synthase subunit G (BcsG) is a Zn(2+)-dependent phosphoethanolamine transferase.,Anderson AC, Burnett AJN, Hiscock L, Maly KE, Weadge JT J Biol Chem. 2020 May 1;295(18):6225-6235. doi: 10.1074/jbc.RA119.011668. Epub, 2020 Mar 9. PMID:32152228<ref>PMID:32152228</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6pcz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Anderson, A C]]		[[Category: Anderson, A C]]

OCA at 09:43, 18 March 2020

OCA — Wed, 18 Mar 2020 09:43:44 GMT

←Older revision		Revision as of 09:43, 18 March 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6pcz ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of the bacterial cellulose synthase subunit G (BcsG) catalytic domain from Escherichia coli, selenomethionine variant==
-		+	<StructureSection load='6pcz' size='340' side='right'caption='[[6pcz]], [[Resolution\|resolution]] 1.44Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6pcz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PCZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PCZ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pcz OCA], [http://pdbe.org/6pcz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pcz RCSB], [http://www.ebi.ac.uk/pdbsum/6pcz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pcz ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Anderson, A C]]
		+	[[Category: Brenner, T]]
		+	[[Category: Weadge, J T]]
		+	[[Category: Biofilm]]
		+	[[Category: Cellulose]]
		+	[[Category: Phosphoethanolamine]]
		+	[[Category: Transferase]]

OCA at 08:56, 17 July 2019

OCA — Wed, 17 Jul 2019 08:56:08 GMT

←Older revision		Revision as of 08:56, 17 July 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pcz is ON HOLD	+	The entry 6pcz is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6pcz" [edit=sysop:move=sysop]

OCA — Wed, 26 Jun 2019 05:18:22 GMT

Protected "6pcz" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:18, 26 June 2019

OCA: New page: '''Unreleased structure''' The entry 6pcz is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Jun 2019 05:18:21 GMT

New page: '''Unreleased structure''' The entry 6pcz is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6pcz is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]