OCA at 07:23, 11 March 2020

OCA — Wed, 11 Mar 2020 07:23:20 GMT

←Older revision		Revision as of 07:23, 11 March 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6pdt is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==cryoEM structure of yeast glucokinase filament==
		+	<StructureSection load='6pdt' size='340' side='right'caption='[[6pdt]], [[Resolution\|resolution]] 3.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6pdt]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PDT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pdt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pdt OCA], [http://pdbe.org/6pdt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pdt RCSB], [http://www.ebi.ac.uk/pdbsum/6pdt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pdt ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HXKG_YEAST HXKG_YEAST]] Two isoenzymes, hexokinase-1 and hexokinase-2, can phosphorylate keto- and aldohexoses in yeast, whereas a third isoenzyme, GLK, is specific for aldohexoses. All glucose phosphorylating enzymes are involved in glucose uptake.<ref>PMID:3072253</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The actin fold is found in cytoskeletal polymers, chaperones, and various metabolic enzymes. Many actin-fold proteins, such as the carbohydrate kinases, do not polymerize. We found that Glk1, a Saccharomyces cerevisiae glucokinase, forms two-stranded filaments with ultrastructure that is distinct from that of cytoskeletal polymers. In cells, Glk1 polymerized upon sugar addition and depolymerized upon sugar withdrawal. Polymerization inhibits enzymatic activity; the Glk1 monomer-polymer equilibrium sets a maximum rate of glucose phosphorylation regardless of Glk1 concentration. A mutation that eliminated Glk1 polymerization alleviated concentration-dependent enzyme inhibition. Yeast containing nonpolymerizing Glk1 were less fit when growing on sugars and more likely to die when refed glucose. Glk1 polymerization arose independently from other actin-related filaments and may allow yeast to rapidly modulate glucokinase activity as nutrient availability changes.

-	~~Authors~~:	+	Polymerization in the actin ATPase clan regulates hexokinase activity in yeast.,Stoddard PR, Lynch EM, Farrell DP, Dosey AM, DiMaio F, Williams TA, Kollman JM, Murray AW, Garner EC Science. 2020 Feb 28;367(6481):1039-1042. doi: 10.1126/science.aay5359. PMID:32108112<ref>PMID:32108112</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6pdt" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Glucokinase]]
		+	[[Category: Large Structures]]
		+	[[Category: Dosey, A M]]
		+	[[Category: Farrell, D P]]
		+	[[Category: Kollman, J M]]
		+	[[Category: Lynch, E M]]
		+	[[Category: Stoddard, P R]]
		+	[[Category: Filament]]
		+	[[Category: Transferase]]

OCA at 04:53, 23 October 2019

OCA — Wed, 23 Oct 2019 04:53:46 GMT

←Older revision		Revision as of 04:53, 23 October 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pdt is ON HOLD	+	The entry 6pdt is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 04:21, 16 October 2019

OCA — Wed, 16 Oct 2019 04:21:36 GMT

←Older revision		Revision as of 04:21, 16 October 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pdt is ON HOLD ~~until Paper Publication~~	+	The entry 6pdt is ON HOLD

	Authors:		Authors:

OCA at 08:56, 17 July 2019

OCA — Wed, 17 Jul 2019 08:56:29 GMT

←Older revision		Revision as of 08:56, 17 July 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pdt is ON HOLD	+	The entry 6pdt is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6pdt" [edit=sysop:move=sysop]

OCA — Wed, 26 Jun 2019 05:20:14 GMT

Protected "6pdt" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:20, 26 June 2019

OCA: New page: '''Unreleased structure''' The entry 6pdt is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Jun 2019 05:20:12 GMT

New page: '''Unreleased structure''' The entry 6pdt is ON HOLD Authors: Description: Category: Unreleased Structures

New page