6pdw - Revision history

OCA at 09:26, 20 March 2024

OCA — Wed, 20 Mar 2024 09:26:09 GMT

←Older revision		Revision as of 09:26, 20 March 2024
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	<SX load='6pdw' size='340' side='right' viewer='molstar' caption='[[6pdw]], [[Resolution\|resolution]] 3.10Å' scene=''>		<SX load='6pdw' size='340' side='right' viewer='molstar' caption='[[6pdw]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pdw]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PDW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6PDW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pdw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PDW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PDW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG~~</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.1Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK~~</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6pdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pdw OCA], [~~http~~://pdbe.org/6pdw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6pdw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6pdw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6pdw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pdw OCA], [https://pdbe.org/6pdw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pdw RCSB], [https://www.ebi.ac.uk/pdbsum/6pdw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pdw ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/G0S654_CHATD G0S654_CHATD]
-	The AAA protein Msp1 extracts mislocalized tail-anchored membrane proteins and targets them for degradation, thus maintaining proper cell organization. How Msp1 selects its substrates and firmly engages them during the energetically unfavorable extraction process remains a mystery. To address this question, we solved cryo-EM structures of Msp1-substrate complexes at near-atomic resolution. Akin to other AAA proteins, Msp1 forms hexameric spirals that translocate substrates through a central pore. A singular hydrophobic substrate recruitment site is exposed at the spiral's seam, which we propose positions the substrate for entry into the pore. There, a tight web of aromatic amino acids grips the substrate in a sequence-promiscuous, hydrophobic milieu. Elements at the intersubunit interfaces coordinate ATP hydrolysis with the subunits' positions in the spiral. We present a comprehensive model of Msp1's mechanism, which follows general architectural principles established for other AAA proteins yet specializes Msp1 for its unique role in membrane protein extraction.	+
-		+
-	~~Structure of the AAA protein Msp1 reveals mechanism of mislocalized membrane protein extraction.,Wang L, Myasnikov A, Pan X, Walter P Elife. 2020 Jan 30;9. pii~~: ~~54031. doi: 10.7554~~/~~eLife.54031. PMID:31999255<ref>PMID:31999255<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6pdw" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</SX>		</SX>
		+	[[Category: Chaetomium thermophilum]]
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Myasnikov, A]]	+	[[Category: Myasnikov A]]
-	[[Category: Pan, X]]	+	[[Category: Pan X]]
-	[[Category: Walter, P]]	+	[[Category: Walter P]]
-	[[Category: Wang, L]]	+	[[Category: Wang L]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Protein quality control]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Tail-anchored protein~~]]	+

OCA at 04:25, 11 April 2020

OCA — Sat, 11 Apr 2020 04:25:44 GMT

←Older revision		Revision as of 04:25, 11 April 2020
Line 3:		Line 3:
	<SX load='6pdw' size='340' side='right' viewer='molstar' caption='[[6pdw]], [[Resolution\|resolution]] 3.10Å' scene=''>		<SX load='6pdw' size='340' side='right' viewer='molstar' caption='[[6pdw]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pdw]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PDW OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6PDW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pdw]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PDW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PDW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6pdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pdw OCA], [http://pdbe.org/6pdw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pdw RCSB], [http://www.ebi.ac.uk/pdbsum/6pdw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pdw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pdw OCA], [http://pdbe.org/6pdw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pdw RCSB], [http://www.ebi.ac.uk/pdbsum/6pdw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pdw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:22, 6 March 2020

OCA — Fri, 06 Mar 2020 23:22:40 GMT

←Older revision		Revision as of 23:22, 6 March 2020
Line 1:		Line 1:

	==Msp1-substrate complex in closed conformation==		==Msp1-substrate complex in closed conformation==
-	<~~StructureSection~~ load='6pdw' size='340' side='right'caption='[[6pdw]], [[Resolution\|resolution]] 3.10Å' scene=''>	+	<SX load='6pdw' size='340' side='right' viewer='molstar' caption='[[6pdw]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6pdw]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PDW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PDW FirstGlance]. <br>		<table><tr><td colspan='2'>[[6pdw]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PDW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PDW FirstGlance]. <br>
Line 20:		Line 20:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Myasnikov, A]]		[[Category: Myasnikov, A]]

OCA at 04:18, 13 February 2020

OCA — Thu, 13 Feb 2020 04:18:57 GMT

←Older revision		Revision as of 04:18, 13 February 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6pdw is ~~ON HOLD until Paper~~ Publication	+	==Msp1-substrate complex in closed conformation==
		+	<StructureSection load='6pdw' size='340' side='right'caption='[[6pdw]], [[Resolution\|resolution]] 3.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6pdw]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PDW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PDW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pdw OCA], [http://pdbe.org/6pdw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pdw RCSB], [http://www.ebi.ac.uk/pdbsum/6pdw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pdw ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The AAA protein Msp1 extracts mislocalized tail-anchored membrane proteins and targets them for degradation, thus maintaining proper cell organization. How Msp1 selects its substrates and firmly engages them during the energetically unfavorable extraction process remains a mystery. To address this question, we solved cryo-EM structures of Msp1-substrate complexes at near-atomic resolution. Akin to other AAA proteins, Msp1 forms hexameric spirals that translocate substrates through a central pore. A singular hydrophobic substrate recruitment site is exposed at the spiral's seam, which we propose positions the substrate for entry into the pore. There, a tight web of aromatic amino acids grips the substrate in a sequence-promiscuous, hydrophobic milieu. Elements at the intersubunit interfaces coordinate ATP hydrolysis with the subunits' positions in the spiral. We present a comprehensive model of Msp1's mechanism, which follows general architectural principles established for other AAA proteins yet specializes Msp1 for its unique role in membrane protein extraction.

-	~~Authors~~:	+	Structure of the AAA protein Msp1 reveals mechanism of mislocalized membrane protein extraction.,Wang L, Myasnikov A, Pan X, Walter P Elife. 2020 Jan 30;9. pii: 54031. doi: 10.7554/eLife.54031. PMID:31999255<ref>PMID:31999255</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6pdw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Myasnikov, A]]
		+	[[Category: Pan, X]]
		+	[[Category: Walter, P]]
		+	[[Category: Wang, L]]
		+	[[Category: Membrane protein]]
		+	[[Category: Protein quality control]]
		+	[[Category: Protein transport]]
		+	[[Category: Tail-anchored protein]]

OCA at 13:12, 29 January 2020

OCA — Wed, 29 Jan 2020 13:12:48 GMT

←Older revision		Revision as of 13:12, 29 January 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pdw is ON HOLD	+	The entry 6pdw is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6pdw" [edit=sysop:move=sysop]

OCA — Wed, 26 Jun 2019 05:20:28 GMT

Protected "6pdw" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:20, 26 June 2019

OCA: New page: '''Unreleased structure''' The entry 6pdw is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Jun 2019 05:20:27 GMT

New page: '''Unreleased structure''' The entry 6pdw is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6pdw is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]