6pft - Revision history

OCA at 07:28, 11 October 2023

OCA — Wed, 11 Oct 2023 07:28:33 GMT

←Older revision		Revision as of 07:28, 11 October 2023
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	<StructureSection load='6pft' size='340' side='right'caption='[[6pft]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='6pft' size='340' side='right'caption='[[6pft]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pft]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aeqvi Aeqvi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PFT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6PFT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pft]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PFT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=OHD:'>OHD</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OHD:{(4Z)-2-[(1S)-1-aminoethyl]-4-[(3-chloro-4-hydroxyphenyl)methylidene]-5-oxo-4,5-dihydro-1H-imidazol-1-yl}acetic+acid'>OHD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6pft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pft OCA], [~~http~~://pdbe.org/6pft PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6pft RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6pft PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6pft ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pft OCA], [https://pdbe.org/6pft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pft RCSB], [https://www.ebi.ac.uk/pdbsum/6pft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pft ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6pft" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6pft" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aeqvi~~]]	+	[[Category: Aequorea victoria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boxer~~, S G~~]]	+	[[Category: Boxer SG]]
-	[[Category: Chang, J]]	+	[[Category: Chang J]]
-	[[Category: Romei~~, M G]]~~	+	[[Category: Romei MG]]
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Green fluorescent protein~~]]	+

OCA at 07:29, 16 October 2019

OCA — Wed, 16 Oct 2019 07:29:31 GMT

←Older revision		Revision as of 07:29, 16 October 2019
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	<StructureSection load='6pft' size='340' side='right'caption='[[6pft]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='6pft' size='340' side='right'caption='[[6pft]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pft]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PFT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pft]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PFT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OHD:'>OHD</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OHD:'>OHD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pft OCA], [http://pdbe.org/6pft PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pft RCSB], [http://www.ebi.ac.uk/pdbsum/6pft PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pft ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pft OCA], [http://pdbe.org/6pft PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pft RCSB], [http://www.ebi.ac.uk/pdbsum/6pft PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pft ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Double-bond photoisomerization in molecules such as the green fluorescent protein (GFP) chromophore can occur either via a volume-demanding one-bond-flip pathway or via a volume-conserving hula-twist pathway. Understanding the factors that determine the pathway of photoisomerization would inform the rational design of photoswitchable GFPs as improved tools for super-resolution microscopy. In this communication, we reveal the photoisomerization pathway of a photoswitchable GFP, rsEGFP2, by solving crystal structures of cis and trans rsEGFP2 containing a monochlorinated chromophore. The position of the chlorine substituent in the trans state breaks the symmetry of the phenolate ring of the chromophore and allows us to distinguish the two pathways. Surprisingly, we find that the pathway depends on the arrangement of protein monomers within the crystal lattice: in a looser packing, the one-bond-flip occurs, whereas, in a tighter packing (7% smaller unit cell size), the hula-twist occurs.
		+
		+	Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins.,Chang J, Romei MG, Boxer SG J Am Chem Soc. 2019 Oct 2;141(39):15504-15508. doi: 10.1021/jacs.9b08356. Epub, 2019 Sep 24. PMID:31533429<ref>PMID:31533429</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6pft" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aeqvi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Boxer, S G]]		[[Category: Boxer, S G]]

OCA at 06:01, 7 August 2019

OCA — Wed, 07 Aug 2019 06:01:15 GMT

←Older revision		Revision as of 06:01, 7 August 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6pft ~~is ON HOLD~~	+	==rsEGFP2 with a chlorinated chromophore in the non-fluorescent off-state==
-		+	<StructureSection load='6pft' size='340' side='right'caption='[[6pft]], [[Resolution\|resolution]] 1.45Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6pft]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PFT FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OHD:'>OHD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pft OCA], [http://pdbe.org/6pft PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pft RCSB], [http://www.ebi.ac.uk/pdbsum/6pft PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pft ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Boxer, S G]]
		+	[[Category: Chang, J]]
		+	[[Category: Romei, M G]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Green fluorescent protein]]

OCA: Protected "6pft" [edit=sysop:move=sysop]

OCA — Wed, 03 Jul 2019 04:16:42 GMT

Protected "6pft" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:16, 3 July 2019

OCA: New page: '''Unreleased structure''' The entry 6pft is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 03 Jul 2019 04:16:40 GMT

New page: '''Unreleased structure''' The entry 6pft is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6pft is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]