OCA at 10:57, 14 June 2023

OCA — Wed, 14 Jun 2023 10:57:44 GMT

←Older revision		Revision as of 10:57, 14 June 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 6pi2 is ~~ON HOLD until Paper~~ Publication	+	==NMR Solution structure of native tachyplesin II peptide==
		+	<StructureSection load='6pi2' size='340' side='right'caption='[[6pi2]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6pi2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Limulus_polyphemus Limulus polyphemus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PI2 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pi2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pi2 OCA], [https://pdbe.org/6pi2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pi2 RCSB], [https://www.ebi.ac.uk/pdbsum/6pi2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pi2 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PPM1_LIMPO PPM1_LIMPO] Significantly inhibits the growth of Gram-negative and Gram-positive bacteria.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tachyplesin I, II and III are host defense peptides from horseshoe crab species with antimicrobial and anticancer activities. They have an amphipathic beta-hairpin structure, are highly positively-charged and differ by only one or two amino acid residues. In this study, we compared the structure and activity of the three tachyplesin peptides alongside their backbone cyclized analogues. We assessed the peptide structures using nuclear magnetic resonance (NMR) spectroscopy, then compared the activity against bacteria (both in the planktonic and biofilm forms) and a panel of cancerous cells. The importance of peptide-lipid interactions was examined using surface plasmon resonance and fluorescence spectroscopy methodologies. Our studies showed that tachyplesin peptides and their cyclic analogues were most potent against Gram-negative bacteria and melanoma cell lines, and showed a preference for binding to negatively-charged lipid membranes. Backbone cyclization did not improve potency, but improved peptide stability in human serum and reduced toxicity toward human red blood cells. Peptide-lipid binding affinity, orientation within the membrane, and ability to disrupt lipid bilayers differed between the cyclized peptide and the parent counterpart. We show that tachyplesin peptides and cyclized analogues have similarly potent antimicrobial and anticancer properties, but that backbone cyclization improves their stability and therapeutic potential.

-	~~Authors:~~ Harvey, ~~P.J.~~, Troeira Henriques, S.~~, Vernen, F~~.	+	Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties.,Vernen F, Harvey PJ, Dias SA, Veiga AS, Huang YH, Craik DJ, Lawrence N, Troeira Henriques S Int J Mol Sci. 2019 Aug 26;20(17):4184. doi: 10.3390/ijms20174184. PMID:31455019<ref>PMID:31455019</ref>

-	~~Description: NMR Solution structure~~ of ~~native tachyplesin II peptide~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Troeira Henriques, S~~]]	+	<div class="pdbe-citations 6pi2" style="background-color:#fffaf0;"></div>
-	[[Category: Harvey~~, P.J~~]]	+
-	[[Category: Vernen, F]]	+	==See Also==
		+	*[[Tachyplesin\|Tachyplesin]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Limulus polyphemus]]
		+	[[Category: Harvey PJ]]
		+	[[Category: Troeira Henriques S]]
		+	[[Category: Vernen F]]

OCA at 04:14, 7 August 2019

OCA — Wed, 07 Aug 2019 04:14:06 GMT

←Older revision		Revision as of 04:14, 7 August 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pi2 is ON HOLD	+	The entry 6pi2 is ON HOLD until Paper Publication

	Authors: Harvey, P.J., Troeira Henriques, S., Vernen, F.		Authors: Harvey, P.J., Troeira Henriques, S., Vernen, F.

OCA: Protected "6pi2" [edit=sysop:move=sysop]

OCA — Wed, 03 Jul 2019 04:22:31 GMT

Protected "6pi2" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:22, 3 July 2019

OCA: New page: '''Unreleased structure''' The entry 6pi2 is ON HOLD Authors: Harvey, P.J., Troeira Henriques, S., Vernen, F. Description: NMR Solution structure of native tachyplesin II peptide [[Cat...

OCA — Wed, 03 Jul 2019 04:22:29 GMT

New page: '''Unreleased structure''' The entry 6pi2 is ON HOLD Authors: Harvey, P.J., Troeira Henriques, S., Vernen, F. Description: NMR Solution structure of native tachyplesin II peptide [[Cat...

New page

'''Unreleased structure'''

The entry 6pi2 is ON HOLD

Authors: Harvey, P.J., Troeira Henriques, S., Vernen, F.

Description: NMR Solution structure of native tachyplesin II peptide
[[Category: Unreleased Structures]]
[[Category: Troeira Henriques, S]]
[[Category: Harvey, P.J]]
[[Category: Vernen, F]]

6pi2 - Revision history

OCA at 10:57, 14 June 2023

OCA at 04:14, 7 August 2019

OCA: Protected "6pi2" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6pi2 is ON HOLD Authors: Harvey, P.J., Troeira Henriques, S., Vernen, F. Description: NMR Solution structure of native tachyplesin II peptide [[Cat...