6pqt - Revision history

OCA at 10:58, 14 June 2023

OCA — Wed, 14 Jun 2023 10:58:26 GMT

←Older revision		Revision as of 10:58, 14 June 2023
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	==N-terminal domain of dynein intermediate chain from Chaetomium thermophilum==		==N-terminal domain of dynein intermediate chain from Chaetomium thermophilum==
-	<StructureSection load='6pqt' size='340' side='right'caption='[[6pqt~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='6pqt' size='340' side='right'caption='[[6pqt]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pqt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Chatd Chatd~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PQT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6PQT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pqt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PQT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PQT FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTHT_0056990 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pqt OCA], [https://pdbe.org/6pqt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pqt RCSB], [https://www.ebi.ac.uk/pdbsum/6pqt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pqt ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6pqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pqt OCA], [~~http~~://pdbe.org/6pqt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6pqt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6pqt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6pqt ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G0SCF1_CHATD G0SCF1_CHATD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 6pqt" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6pqt" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dynein 3D structures\|Dynein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chatd~~]]	+	[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Barbar, E]]	+	[[Category: Barbar E]]
-	[[Category: Loening~~, N M]]~~	+	[[Category: Loening NM]]
-	~~[[Category: Dynein]]~~	+
-	~~[[Category: Idp]]~~	+
-	~~[[Category: Intermediate chain]]~~	+
-	~~[[Category: Intrinsically disordered protein]]~~	+
-	~~[[Category: Motor protein~~]]	+

OCA at 07:03, 2 September 2020

OCA — Wed, 02 Sep 2020 07:03:21 GMT

←Older revision		Revision as of 07:03, 2 September 2020
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	==N-terminal domain of dynein intermediate chain from Chaetomium thermophilum==		==N-terminal domain of dynein intermediate chain from Chaetomium thermophilum==
-	<StructureSection load='6pqt' size='340' side='right'caption='[[6pqt]]' scene=''>	+	<StructureSection load='6pqt' size='340' side='right'caption='[[6pqt]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PQT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PQT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pqt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PQT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PQT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pqt OCA], [http://pdbe.org/6pqt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pqt RCSB], [http://www.ebi.ac.uk/pdbsum/6pqt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pqt ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTHT_0056990 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pqt OCA], [http://pdbe.org/6pqt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pqt RCSB], [http://www.ebi.ac.uk/pdbsum/6pqt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pqt ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytoplasmic dynein is a eukaryotic motor protein complex that, along with its regulatory protein dynactin, is essential to the transport of organelles within cells. The interaction of dynein with dynactin is regulated by binding between the intermediate chain (IC) subunit of dynein and the p150(Glued) subunit of dynactin. Even though in the rat versions of these proteins this interaction primarily involves the single alpha-helix region at the N-terminus of the IC, in Drosophila and yeast ICs the removal of a nascent helix (H2) downstream of the single alpha-helix considerably diminishes IC-p150(Glued) complex stability. We find that for ICs from various species, there is a correlation between disorder in H2 and its contribution to binding affinity, and that sequence variations in H2 that do not change the level of disorder show similar binding behavior. Analysis of the structure and interactions of the IC from Chaetomium thermophilum demonstrates that the H2 region of C. thermophilum IC has a low helical propensity and establishes that H2 binds directly to the coiled-coil 1B (CC1B) domain of p150(Glued), thus explaining why H2 is necessary for tight binding. Isothermal titration calorimetry, circular dichroism, and NMR studies of smaller CC1B constructs localize the region of CC1B most essential for a tight interaction with IC. These results suggest that it is the level of disorder in H2 of IC along with its charge, rather than sequence specificity, that underlie its importance in initiating tight IC-p150(Glued) complex formation. We speculate that the nascent H2 helix may provide conformational flexibility to initiate binding, whereas those species that have a fully folded H2 have co-opted an alternative mechanism for promoting p150(Glued) binding.
		+
		+	Interplay of Disorder and Sequence Specificity in the Formation of Stable Dynein-Dynactin Complexes.,Loening NM, Saravanan S, Jespersen NE, Jara K, Barbar E Biophys J. 2020 Aug 5. pii: S0006-3495(20)30587-7. doi:, 10.1016/j.bpj.2020.07.023. PMID:32814057<ref>PMID:32814057</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6pqt" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chatd]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Barbar E]]	+	[[Category: Barbar, E]]
-	[[Category: Loening NM]]	+	[[Category: Loening, N M]]
		+	[[Category: Dynein]]
		+	[[Category: Idp]]
		+	[[Category: Intermediate chain]]
		+	[[Category: Intrinsically disordered protein]]
		+	[[Category: Motor protein]]

OCA at 06:18, 19 August 2020

OCA — Wed, 19 Aug 2020 06:18:20 GMT

←Older revision		Revision as of 06:18, 19 August 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6pqt ~~is ON HOLD until Paper Publication~~	+	==N-terminal domain of dynein intermediate chain from Chaetomium thermophilum==
-		+	<StructureSection load='6pqt' size='340' side='right'caption='[[6pqt]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PQT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PQT FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pqt OCA], [http://pdbe.org/6pqt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pqt RCSB], [http://www.ebi.ac.uk/pdbsum/6pqt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pqt ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Barbar E]]
		+	[[Category: Loening NM]]

OCA at 04:29, 24 July 2019

OCA — Wed, 24 Jul 2019 04:29:14 GMT

←Older revision		Revision as of 04:29, 24 July 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pqt is ON HOLD	+	The entry 6pqt is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6pqt" [edit=sysop:move=sysop]

OCA — Wed, 17 Jul 2019 09:08:35 GMT

Protected "6pqt" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:08, 17 July 2019

OCA: New page: '''Unreleased structure''' The entry 6pqt is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 17 Jul 2019 09:08:32 GMT

New page: '''Unreleased structure''' The entry 6pqt is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6pqt is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]