6pw7 - Revision history

OCA at 09:21, 27 November 2019

OCA — Wed, 27 Nov 2019 09:21:36 GMT

←Older revision		Revision as of 09:21, 27 November 2019
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	<StructureSection load='6pw7' size='340' side='right'caption='[[6pw7]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='6pw7' size='340' side='right'caption='[[6pw7]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pw7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PW7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PW7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pw7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PW7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PW7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">stim-1, Y55B1BM.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pw7 OCA], [http://pdbe.org/6pw7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pw7 RCSB], [http://www.ebi.ac.uk/pdbsum/6pw7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pw7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pw7 OCA], [http://pdbe.org/6pw7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pw7 RCSB], [http://www.ebi.ac.uk/pdbsum/6pw7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pw7 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Caeel]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Back, S I]]		[[Category: Back, S I]]

OCA at 11:09, 13 November 2019

OCA — Wed, 13 Nov 2019 11:09:12 GMT

←Older revision		Revision as of 11:09, 13 November 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6pw7 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==X-ray crystal structure of C. elegans STIM EF-SAM domain==
		+	<StructureSection load='6pw7' size='340' side='right'caption='[[6pw7]], [[Resolution\|resolution]] 1.89Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6pw7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PW7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PW7 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pw7 OCA], [http://pdbe.org/6pw7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pw7 RCSB], [http://www.ebi.ac.uk/pdbsum/6pw7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pw7 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/STIM1_CAEEL STIM1_CAEEL]] Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor which upon Ca(2+) depletion, activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, orai-1. Essential for Ca (2+) and IP3-dependent contractile activity of gonad sheath cells and spermatheca. Essential for fertility. Does not play a role in posterior body wall muscle contraction (pBoc) rhythmicity, intestinal cell oscillatory Ca(2+) signaling or intestinal ER Ca(2+) hemeostasis.<ref>PMID:16966474</ref> <ref>PMID:17218360</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Store operated calcium (Ca(2+)) entry (SOCE) is the process whereby endoplasmic reticulum (ER) Ca(2+) store depletion causes Orai1-composed Ca(2+) channels on the plasma membrane (PM) to open, mediating a rise in cytosolic Ca(2+) levels. Stromal interaction molecules (STIM)s are the proteins that directly sense ER Ca(2+) content and gate Orai1 channels due to store depletion. The trigger for STIM activation is Ca(2+) unbinding from the ER lumen-oriented domains, which consist of a non-conserved amino (N) terminal region and EF-hand and sterile alpha motif (SAM) domains (EF-SAM), highly conserved from humans to Caenorhabditis elegans. Solution NMR structures of the human EF-SAM domains have been determined at high Ca(2+) concentrations; however, no direct structural view of the Ca(2+) binding mode has been elucidated. Further, no atomic resolution data currently exists on EF-SAM at low Ca(2+) levels. Here, we determined the X-ray crystal structure of the C. elegans STIM luminal domain, revealing that EF-SAM binds a single Ca(2+) ion with pentagonal bipyramidal geometry and an ancillary alpha-helix formed by the N-terminal region acts as a brace to stabilize EF-SAM. Using solution NMR, we observed EF-hand domain unfolding and a conformational exchange between folded and unfolded states involving the ancillary alpha-helix and the canonical EF-hand in low Ca(2+). Remarkably, we also detected an alpha-helix (+Ca(2+)) to beta-strand (-Ca(2+)) transition at the terminal SAM domain alpha-helix. Collectively, our analyses indicate that one canonically bound Ca(2+) ion is sufficient to stabilize the quiescent luminal domain structure, precluding unfolding, conformational exchange and secondary structure transformation.

-	~~Authors~~:	+	Coordination of a single calcium ion in the EF-hand maintains the off state of the stromal interaction molecule luminal domain.,Enomoto M, Nishikawa T, Back SI, Ishiyama N, Zheng L, Stathopulos PB, Ikura M J Mol Biol. 2019 Oct 15. pii: S0022-2836(19)30594-7. doi:, 10.1016/j.jmb.2019.10.003. PMID:31626806<ref>PMID:31626806</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6pw7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Back, S I]]
		+	[[Category: Enomoto, M]]
		+	[[Category: Ikura, M]]
		+	[[Category: Ishiyama, N]]
		+	[[Category: Nishikawa, T]]
		+	[[Category: Stathopulos, P B]]
		+	[[Category: Zheng, L]]
		+	[[Category: Calcium release-activated channel]]
		+	[[Category: Ef-hand]]
		+	[[Category: Sam-domain]]
		+	[[Category: Signaling protein]]

OCA at 07:14, 2 October 2019

OCA — Wed, 02 Oct 2019 07:14:47 GMT

←Older revision		Revision as of 07:14, 2 October 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pw7 is ON HOLD	+	The entry 6pw7 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6pw7" [edit=sysop:move=sysop]

OCA — Wed, 31 Jul 2019 04:48:06 GMT

Protected "6pw7" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:48, 31 July 2019

OCA: New page: '''Unreleased structure''' The entry 6pw7 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 31 Jul 2019 04:48:04 GMT

New page: '''Unreleased structure''' The entry 6pw7 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6pw7 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]