6pwj - Revision history

OCA at 14:55, 13 March 2024

OCA — Wed, 13 Mar 2024 14:55:44 GMT

←Older revision		Revision as of 14:55, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6pwj' size='340' side='right'caption='[[6pwj]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='6pwj' size='340' side='right'caption='[[6pwj]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pwj]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Vibrio_cholerae_2010el~~-1786 Vibrio cholerae ~~2010el~~-1786]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6PWJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pwj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_str._2010EL-1786 Vibrio cholerae O1 str. 2010EL-1786]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PWJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6pwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwj OCA], [~~http~~://pdbe.org/6pwj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6pwj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6pwj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwj ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwj OCA], [https://pdbe.org/6pwj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pwj RCSB], [https://www.ebi.ac.uk/pdbsum/6pwj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwj ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A0A0H3AFX3_VIBC3 A0A0H3AFX3_VIBC3]
-	The dinucleotide second messenger c-di-GMP has emerged as a central regulator of reversible cell attachment during bacterial biofilm formation. A prominent cell adhesion mechanism first identified in pseudomonads combines two c-di-GMP-mediated processes: transcription of a large adhesin and its cell surface display via posttranslational proteolytic control. Here, we characterize an orthologous c-di-GMP effector system and show that it is operational in Vibrio cholerae, where it regulates two distinct classes of adhesins. Through structural analyses, we reveal a conserved autoinhibition mechanism of the c-di-GMP receptor that controls adhesin proteolysis and present a structure of a c-di-GMP-bound receptor module. We further establish functionality of the periplasmic protease controlled by the receptor against the two adhesins. Finally, transcription and functional assays identify physiological roles of both c-di-GMP-regulated adhesins in surface attachment and biofilm formation. Together, our studies highlight the conservation of a highly efficient signaling effector circuit for the control of cell surface adhesin expression and its versatility by revealing strain-specific variations.IMPORTANCE Vibrio cholerae, the causative agent of the diarrheal disease cholera, benefits from a sessile biofilm lifestyle that enhances survival outside the host but also contributes to host colonization and infectivity. The bacterial second messenger c-di-GMP has been identified as a central regulator of biofilm formation, including in V. cholerae; however, our understanding of the pathways that contribute to this process is incomplete. Here, we define a conserved signaling system that controls the stability of large adhesion proteins at the cell surface of V. cholerae, which are important for cell attachment and biofilm formation. Insight into the regulatory circuit underlying biofilm formation may inform targeted strategies to interfere with a process that renders this bacterium remarkably adaptable to changing environments.	+
-		+
-	A Conserved Regulatory Circuit Controls Large Adhesins in Vibrio cholerae.,Kitts G, Giglio KM, Zamorano-Sanchez D, Park JH, Townsley L, Cooley RB, Wucher BR, Klose KE, Nadell CD, Yildiz FH, Sondermann H mBio. 2019 Dec 3;10(6). pii: mBio.02822-19. doi: 10.1128/~~mBio.02822-19. PMID:31796544<ref>PMID:31796544<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6pwj" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Vibrio cholerae ~~2010el~~-1786]]	+	[[Category: Vibrio cholerae O1 str. 2010EL-1786]]
-	[[Category: Cooley~~, R B~~]]	+	[[Category: Cooley RB]]
-	[[Category: Giglio~~, K M~~]]	+	[[Category: Giglio KM]]
-	[[Category: Sondermann, H]]	+	[[Category: Sondermann H]]
-	~~[[Category: Biofilm formation]]~~	+
-	~~[[Category: C-di-gmp signaling]]~~	+
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Receptor]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 06:03, 22 April 2020

OCA — Wed, 22 Apr 2020 06:03:44 GMT

←Older revision		Revision as of 06:03, 22 April 2020
Line 3:		Line 3:
	<StructureSection load='6pwj' size='340' side='right'caption='[[6pwj]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='6pwj' size='340' side='right'caption='[[6pwj]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pwj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae_2010el-1786 Vibrio cholerae 2010el-1786]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWJ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6PWJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pwj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae_2010el-1786 Vibrio cholerae 2010el-1786]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PWJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6pwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwj OCA], [http://pdbe.org/6pwj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pwj RCSB], [http://www.ebi.ac.uk/pdbsum/6pwj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwj OCA], [http://pdbe.org/6pwj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pwj RCSB], [http://www.ebi.ac.uk/pdbsum/6pwj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwj ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The dinucleotide second messenger c-di-GMP has emerged as a central regulator of reversible cell attachment during bacterial biofilm formation. A prominent cell adhesion mechanism first identified in pseudomonads combines two c-di-GMP-mediated processes: transcription of a large adhesin and its cell surface display via posttranslational proteolytic control. Here, we characterize an orthologous c-di-GMP effector system and show that it is operational in Vibrio cholerae, where it regulates two distinct classes of adhesins. Through structural analyses, we reveal a conserved autoinhibition mechanism of the c-di-GMP receptor that controls adhesin proteolysis and present a structure of a c-di-GMP-bound receptor module. We further establish functionality of the periplasmic protease controlled by the receptor against the two adhesins. Finally, transcription and functional assays identify physiological roles of both c-di-GMP-regulated adhesins in surface attachment and biofilm formation. Together, our studies highlight the conservation of a highly efficient signaling effector circuit for the control of cell surface adhesin expression and its versatility by revealing strain-specific variations.IMPORTANCE Vibrio cholerae, the causative agent of the diarrheal disease cholera, benefits from a sessile biofilm lifestyle that enhances survival outside the host but also contributes to host colonization and infectivity. The bacterial second messenger c-di-GMP has been identified as a central regulator of biofilm formation, including in V. cholerae; however, our understanding of the pathways that contribute to this process is incomplete. Here, we define a conserved signaling system that controls the stability of large adhesion proteins at the cell surface of V. cholerae, which are important for cell attachment and biofilm formation. Insight into the regulatory circuit underlying biofilm formation may inform targeted strategies to interfere with a process that renders this bacterium remarkably adaptable to changing environments.
		+
		+	A Conserved Regulatory Circuit Controls Large Adhesins in Vibrio cholerae.,Kitts G, Giglio KM, Zamorano-Sanchez D, Park JH, Townsley L, Cooley RB, Wucher BR, Klose KE, Nadell CD, Yildiz FH, Sondermann H mBio. 2019 Dec 3;10(6). pii: mBio.02822-19. doi: 10.1128/mBio.02822-19. PMID:31796544<ref>PMID:31796544</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6pwj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:21, 27 November 2019

OCA — Wed, 27 Nov 2019 09:21:46 GMT

←Older revision		Revision as of 09:21, 27 November 2019
Line 3:		Line 3:
	<StructureSection load='6pwj' size='340' side='right'caption='[[6pwj]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='6pwj' size='340' side='right'caption='[[6pwj]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pwj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PWJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pwj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae_2010el-1786 Vibrio cholerae 2010el-1786]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PWJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwj OCA], [http://pdbe.org/6pwj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pwj RCSB], [http://www.ebi.ac.uk/pdbsum/6pwj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwj OCA], [http://pdbe.org/6pwj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pwj RCSB], [http://www.ebi.ac.uk/pdbsum/6pwj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwj ProSAT]</span></td></tr>
Line 10:		Line 10:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Vibrio cholerae 2010el-1786]]
	[[Category: Cooley, R B]]		[[Category: Cooley, R B]]
	[[Category: Giglio, K M]]		[[Category: Giglio, K M]]

OCA at 05:23, 10 October 2019

OCA — Thu, 10 Oct 2019 05:23:18 GMT

←Older revision		Revision as of 05:23, 10 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6pwj ~~is ON HOLD~~	+	==Vibrio cholerae LapD S helix-GGDEF-EAL (apo)==
-		+	<StructureSection load='6pwj' size='340' side='right'caption='[[6pwj]], [[Resolution\|resolution]] 2.70Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6pwj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PWJ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwj OCA], [http://pdbe.org/6pwj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pwj RCSB], [http://www.ebi.ac.uk/pdbsum/6pwj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwj ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Cooley, R B]]
		+	[[Category: Giglio, K M]]
		+	[[Category: Sondermann, H]]
		+	[[Category: Biofilm formation]]
		+	[[Category: C-di-gmp signaling]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Receptor]]
		+	[[Category: Signaling protein]]

OCA: Protected "6pwj" [edit=sysop:move=sysop]

OCA — Wed, 31 Jul 2019 04:49:29 GMT

Protected "6pwj" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:49, 31 July 2019

OCA: New page: '''Unreleased structure''' The entry 6pwj is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 31 Jul 2019 04:49:27 GMT

New page: '''Unreleased structure''' The entry 6pwj is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6pwj is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]