6pwu - Revision history

OCA at 07:09, 16 March 2022

OCA — Wed, 16 Mar 2022 07:09:38 GMT

←Older revision		Revision as of 07:09, 16 March 2022
Line 3:		Line 3:
	<SX load='6pwu' size='340' side='right' viewer='molstar' caption='[[6pwu]], [[Resolution\|resolution]] 6.20Å' scene=''>		<SX load='6pwu' size='340' side='right' viewer='molstar' caption='[[6pwu]], [[Resolution\|resolution]] 6.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pwu]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/9hiv1 9hiv1] and [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6PWU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pwu]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/9hiv1 9hiv1] and [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PWU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">env ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">env ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6pwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwu OCA], [~~http~~://pdbe.org/6pwu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6pwu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6pwu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwu OCA], [https://pdbe.org/6pwu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pwu RCSB], [https://www.ebi.ac.uk/pdbsum/6pwu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwu ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:51, 9 September 2020

OCA — Wed, 09 Sep 2020 09:51:29 GMT

←Older revision		Revision as of 09:51, 9 September 2020
Line 3:		Line 3:
	<SX load='6pwu' size='340' side='right' viewer='molstar' caption='[[6pwu]], [[Resolution\|resolution]] 6.20Å' scene=''>		<SX load='6pwu' size='340' side='right' viewer='molstar' caption='[[6pwu]], [[Resolution\|resolution]] 6.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pwu]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PWU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pwu]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PWU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <~~scene name~~='~~pdbligand~~=~~NAG~~:~~N-ACETYL-D-GLUCOSAMINE'~~>~~NAG~~</~~scene~~></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">env ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwu OCA], [http://pdbe.org/6pwu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pwu RCSB], [http://www.ebi.ac.uk/pdbsum/6pwu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwu OCA], [http://pdbe.org/6pwu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pwu RCSB], [http://www.ebi.ac.uk/pdbsum/6pwu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwu ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The HIV-1 envelope protein (Env) is the target of neutralizing antibodies and the template for vaccine immunogen design. The dynamic conformational equilibrium of trimeric Env influences its antigenicity and potential immunogenicity. Antibodies that bind at the trimer apex stabilize a "closed" conformation characteristic of the most difficult to neutralize isolates. A goal of vaccine development is therefore to mimic the closed conformation in a designed immunogen. A disulfide-stabilized, trimeric Env ectodomain-the "SOSIP" construct-has many of the relevant properties; it is also particularly suitable for structure determination. Some single-molecule studies have, however, suggested that the SOSIP trimer is not a good representation of Env on the surface of a virion or an infected cell. We isolated Env (fully cleaved to gp120 and gp41) from the surface of expressing cells using tagged, apex-binding Fab PG16 and determined the structure of the PG16-Env complex by cryo-EM to an overall resolution of 4.6 A. Placing the only purification tag on the Fab ensured that the isolated Env was continuously stabilized in its closed, native conformation. The Env structure in this complex corresponds closely to the SOSIP structures determined by both x-ray crystallography and cryo-EM. Although the membrane-interacting elements are not resolved in our reconstruction, we can make inferences about the connection between ectodomain and membrane-proximal external region (MPER) by reference to the published cryo-tomography structure of an Env "spike" and the NMR structure of the MPER-transmembrane segment. We discuss these results in view of the conflicting interpretations in the literature.
		+
		+	Cryo-EM Structure of Full-length HIV-1 Env Bound With the Fab of Antibody PG16.,Pan J, Peng H, Chen B, Harrison SC J Mol Biol. 2020 Feb 14;432(4):1158-1168. doi: 10.1016/j.jmb.2019.11.028. Epub, 2020 Jan 11. PMID:31931014<ref>PMID:31931014</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6pwu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[3D structures of human antibody\|3D structures of human antibody]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</SX>		</SX>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chen, B]]		[[Category: Chen, B]]

OCA at 04:41, 11 April 2020

OCA — Sat, 11 Apr 2020 04:41:42 GMT

←Older revision		Revision as of 04:41, 11 April 2020
Line 3:		Line 3:
	<SX load='6pwu' size='340' side='right' viewer='molstar' caption='[[6pwu]], [[Resolution\|resolution]] 6.20Å' scene=''>		<SX load='6pwu' size='340' side='right' viewer='molstar' caption='[[6pwu]], [[Resolution\|resolution]] 6.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pwu]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWU OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6PWU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pwu]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PWU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6pwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwu OCA], [http://pdbe.org/6pwu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pwu RCSB], [http://www.ebi.ac.uk/pdbsum/6pwu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwu OCA], [http://pdbe.org/6pwu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pwu RCSB], [http://www.ebi.ac.uk/pdbsum/6pwu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwu ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 23:28, 6 March 2020

OCA — Fri, 06 Mar 2020 23:28:37 GMT

←Older revision		Revision as of 23:28, 6 March 2020
Line 1:		Line 1:

	==Structure of full-length, fully glycosylated, non-modified HIV-1 gp160 bound to PG16 Fab==		==Structure of full-length, fully glycosylated, non-modified HIV-1 gp160 bound to PG16 Fab==
-	<~~StructureSection~~ load='6pwu' size='340' side='right'caption='[[6pwu]], [[Resolution\|resolution]] 6.20Å' scene=''>	+	<SX load='6pwu' size='340' side='right' viewer='molstar' caption='[[6pwu]], [[Resolution\|resolution]] 6.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6pwu]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PWU FirstGlance]. <br>		<table><tr><td colspan='2'>[[6pwu]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PWU FirstGlance]. <br>
Line 8:		Line 8:
	</table>		</table>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chen, B]]		[[Category: Chen, B]]

OCA at 09:20, 26 February 2020

OCA — Wed, 26 Feb 2020 09:20:36 GMT

←Older revision		Revision as of 09:20, 26 February 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6pwu is ON HOLD~~	+	==Structure of full-length, fully glycosylated, non-modified HIV-1 gp160 bound to PG16 Fab==
-		+	<StructureSection load='6pwu' size='340' side='right'caption='[[6pwu]], [[Resolution\|resolution]] 6.20Å' scene=''>
-	~~Authors: Pan, J., Chen, B., Harrison, S.C.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6pwu]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PWU FirstGlance]. <br>
-	~~Description:~~ Structure of full-length, fully glycosylated, non-modified HIV-1 gp160 bound to PG16 Fab	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwu OCA], [http://pdbe.org/6pwu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pwu RCSB], [http://www.ebi.ac.uk/pdbsum/6pwu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwu ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Harrison~~, S.C]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Chen, B]]		[[Category: Chen, B]]
		+	[[Category: Harrison, S C]]
	[[Category: Pan, J]]		[[Category: Pan, J]]
		+	[[Category: Env]]
		+	[[Category: Hiv-1]]
		+	[[Category: Viral protein]]

OCA: Protected "6pwu" [edit=sysop:move=sysop]

OCA — Wed, 31 Jul 2019 04:50:24 GMT

Protected "6pwu" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:50, 31 July 2019

OCA: New page: '''Unreleased structure''' The entry 6pwu is ON HOLD Authors: Pan, J., Chen, B., Harrison, S.C. Description: Structure of full-length, fully glycosylated, non-modified HIV-1 gp160 boun...

OCA — Wed, 31 Jul 2019 04:50:22 GMT

New page: '''Unreleased structure''' The entry 6pwu is ON HOLD Authors: Pan, J., Chen, B., Harrison, S.C. Description: Structure of full-length, fully glycosylated, non-modified HIV-1 gp160 boun...

New page

'''Unreleased structure'''

The entry 6pwu is ON HOLD

Authors: Pan, J., Chen, B., Harrison, S.C.

Description: Structure of full-length, fully glycosylated, non-modified HIV-1 gp160 bound to PG16 Fab
[[Category: Unreleased Structures]]
[[Category: Harrison, S.C]]
[[Category: Chen, B]]
[[Category: Pan, J]]