OCA at 11:51, 24 January 2024

OCA — Wed, 24 Jan 2024 11:51:54 GMT

←Older revision		Revision as of 11:51, 24 January 2024
Line 1:		Line 1:

	==The structure of the Mo-insertase domain Cnx1E (variant S269DD274S) from Arabidopsis thaliana in complex with Moco-AMP==		==The structure of the Mo-insertase domain Cnx1E (variant S269DD274S) from Arabidopsis thaliana in complex with Moco-AMP==
-	<StructureSection load='6q32' size='340' side='right'caption='[[6q32]]' scene=''>	+	<StructureSection load='6q32' size='340' side='right'caption='[[6q32]], [[Resolution\|resolution]] 1.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Q32 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6q32]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Q32 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6q32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q32 OCA], [https://pdbe.org/6q32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6q32 RCSB], [https://www.ebi.ac.uk/pdbsum/6q32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6q32 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.39Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NWS:Moco-AMP'>NWS</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6q32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q32 OCA], [https://pdbe.org/6q32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6q32 RCSB], [https://www.ebi.ac.uk/pdbsum/6q32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6q32 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CNX1_ARATH CNX1_ARATH] Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.<ref>PMID:15504727</ref> <ref>PMID:16636046</ref> <ref>PMID:12590921</ref> <ref>PMID:15306815</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The molybdenum cofactor (Moco) is found in the active site of numerous important enzymes that are critical to biological processes. The bidentate ligand that chelates molybdenum in Moco is the pyranopterin dithiolene (molybdopterin, MPT). However, neither the mechanism of molybdate insertion into MPT nor the structure of Moco prior to its insertion into pyranopterin molybdenum enzymes is known. Here, we report this final maturation step, where adenylated MPT (MPT-AMP) and molybdate are the substrates. X-ray crystallography of the Arabidopsis thaliana Mo-insertase variant Cnx1E S269D D274S identified adenylated Moco (Moco-AMP) as an unexpected intermediate in this reaction sequence. X-ray absorption spectroscopy revealed the first coordination sphere geometry of Moco trapped in the Cnx1E active site. We have used this structural information to deduce a mechanism for molybdate insertion into MPT-AMP. Given their high degree of structural and sequence similarity, we suggest that this mechanism is employed by all eukaryotic Mo-insertases.
		+
		+	Mechanism of molybdate insertion into pterin-based molybdenum cofactors.,Probst C, Yang J, Krausze J, Hercher TW, Richers CP, Spatzal T, Kc K, Giles LJ, Rees DC, Mendel RR, Kirk ML, Kruse T Nat Chem. 2021 Aug;13(8):758-765. doi: 10.1038/s41557-021-00714-1. Epub 2021 Jun , 28. PMID:34183818<ref>PMID:34183818</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6q32" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Krausze J]]		[[Category: Krausze J]]

OCA at 09:55, 17 February 2021

OCA — Wed, 17 Feb 2021 09:55:46 GMT

←Older revision		Revision as of 09:55, 17 February 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 6q32 ~~is ON HOLD until Paper Publication~~	+	==The structure of the Mo-insertase domain Cnx1E (variant S269DD274S) from Arabidopsis thaliana in complex with Moco-AMP==
-		+	<StructureSection load='6q32' size='340' side='right'caption='[[6q32]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Q32 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6q32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q32 OCA], [https://pdbe.org/6q32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6q32 RCSB], [https://www.ebi.ac.uk/pdbsum/6q32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6q32 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Krausze J]]

OCA: Protected "6q32" [edit=sysop:move=sysop]

OCA — Wed, 12 Dec 2018 05:29:40 GMT

Protected "6q32" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:29, 12 December 2018

OCA: New page: '''Unreleased structure''' The entry 6q32 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 12 Dec 2018 05:29:38 GMT

New page: '''Unreleased structure''' The entry 6q32 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6q32 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

6q32 - Revision history

OCA at 11:51, 24 January 2024

OCA at 09:55, 17 February 2021

OCA: Protected "6q32" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6q32 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures