6q5g - Revision history

OCA at 12:14, 1 January 2020

OCA — Wed, 01 Jan 2020 12:14:14 GMT

←Older revision		Revision as of 12:14, 1 January 2020
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	<StructureSection load='6q5g' size='340' side='right'caption='[[6q5g]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6q5g' size='340' side='right'caption='[[6q5g]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6q5g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q5G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6Q5G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6q5g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifab Bifab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q5G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6Q5G FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6h0h\|6h0h]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6h0h\|6h0h]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DU497_02550 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=552531 BIFAB])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6q5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q5g OCA], [http://pdbe.org/6q5g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6q5g RCSB], [http://www.ebi.ac.uk/pdbsum/6q5g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6q5g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6q5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q5g OCA], [http://pdbe.org/6q5g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6q5g RCSB], [http://www.ebi.ac.uk/pdbsum/6q5g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6q5g ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bifidobacteria are exposed to substantial amounts of dietary beta-galactosides. Distinctive preferences for growth on different beta-galactosides are observed within Bifidobacterium members, but the basis of these preferences remains unclear. We previously described the first beta-(1,6)/(1,3)-galactosidase from Bifidobacterium animalis subsp. lactis Bl-04. This enzyme is relatively promiscuous, exhibiting only 5-fold higher efficiency on the preferred beta-(1,6)-galactobiose than the beta-(1,4) isomer. Here, we characterize the solute-binding protein (Bal6GBP) that governs the specificity of the ABC transporter encoded by the same beta-galactoside utilization locus. We observed that although Bal6GBP recognizes both beta-(1,6)- and beta-(1,4)-galactobiose, Bal6GBP has a 1630-fold higher selectivity for the former, reflected in dramatic differences in growth, with several hours lag on less preferred beta-(1,4)- and beta-(1,3)-galactobiose. Experiments performed in the presence of varying proportions of beta-(1,4)/beta-(1,6)-galactobioses indicated that the preferred substrate was preferentially depleted from the culture supernatant. This established that the poor growth on the nonpreferred beta-(1,4) was due to inefficient uptake. We solved the structure of Bal6GBP in complex with beta-(1,6)-galactobiose at 1.39 A resolution, revealing the structural basis of this strict selectivity. Moreover, we observed a close evolutionary relationship with the human milk disaccharide lacto-N-biose-binding protein from Bifidobacterium longum, indicating that the recognition of the nonreducing galactosyl is essentially conserved, whereas the adjacent position is diversified to fit different glycosidic linkages and monosaccharide residues. These findings indicate that oligosaccharide uptake has a pivotal role in governing selectivity for distinct growth substrates and have uncovered evolutionary trajectories that shape the diversification of sugar uptake proteins within Bifidobacterium.
		+
		+	Substrate preference of an ABC importer corresponds to selective growth on beta-(1,6)-galactosides in Bifidobacterium animalis subsp. lactis.,Theilmann MC, Fredslund F, Svensson B, Lo Leggio L, Abou Hachem M J Biol Chem. 2019 Aug 2;294(31):11701-11711. doi: 10.1074/jbc.RA119.008843. Epub , 2019 Jun 11. PMID:31186348<ref>PMID:31186348</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6q5g" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bifab]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Fredslund, F]]		[[Category: Fredslund, F]]

OCA at 06:14, 19 June 2019

OCA — Wed, 19 Jun 2019 06:14:33 GMT

←Older revision		Revision as of 06:14, 19 June 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6q5g is ON HOLD~~	+	==The ABC transporter associated binding protein from B. animalis subsp. lactis Bl-04 without ligand. SeMet variant==
-		+	<StructureSection load='6q5g' size='340' side='right'caption='[[6q5g]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors: Fredslund, F., Lo Leggio, L.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6q5g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q5G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6Q5G FirstGlance]. <br>
-	~~Description:~~ The ABC transporter associated binding protein from B. animalis subsp. lactis Bl-04 without ligand. SeMet variant	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6h0h\|6h0h]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6q5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q5g OCA], [http://pdbe.org/6q5g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6q5g RCSB], [http://www.ebi.ac.uk/pdbsum/6q5g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6q5g ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Fredslund, F]]		[[Category: Fredslund, F]]
-	[[Category: Lo Leggio, L]]	+	[[Category: Leggio, L Lo]]
		+	[[Category: Abc transporter]]
		+	[[Category: Complex]]
		+	[[Category: Phasing]]
		+	[[Category: Sugar binding protein]]

OCA: Protected "6q5g" [edit=sysop:move=sysop]

OCA — Wed, 19 Dec 2018 06:43:36 GMT

Protected "6q5g" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:43, 19 December 2018

OCA: New page: '''Unreleased structure''' The entry 6q5g is ON HOLD Authors: Fredslund, F., Lo Leggio, L. Description: The ABC transporter associated binding protein from B. animalis subsp. lactis Bl...

OCA — Wed, 19 Dec 2018 06:43:35 GMT

New page: '''Unreleased structure''' The entry 6q5g is ON HOLD Authors: Fredslund, F., Lo Leggio, L. Description: The ABC transporter associated binding protein from B. animalis subsp. lactis Bl...

New page

'''Unreleased structure'''

The entry 6q5g is ON HOLD

Authors: Fredslund, F., Lo Leggio, L.

Description: The ABC transporter associated binding protein from B. animalis subsp. lactis Bl-04 without ligand. SeMet variant
[[Category: Unreleased Structures]]
[[Category: Fredslund, F]]
[[Category: Lo Leggio, L]]