6q6v - Revision history

OCA at 11:54, 24 January 2024

OCA — Wed, 24 Jan 2024 11:54:20 GMT

←Older revision		Revision as of 11:54, 24 January 2024
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	==Crystal structure (trigonal form) of C36S mutant of thioredoxin h1 from Chlamydomonas reinhardtii==		==Crystal structure (trigonal form) of C36S mutant of thioredoxin h1 from Chlamydomonas reinhardtii==
-	<StructureSection load='6q6v' size='340' side='right' caption='[[6q6v]], [[Resolution\|resolution]] 1.22Å' scene=''>	+	<StructureSection load='6q6v' size='340' side='right'caption='[[6q6v]], [[Resolution\|resolution]] 1.22Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6q6v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q6V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6Q6V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6q6v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Q6V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.22Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6q6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q6v OCA], [~~http~~://pdbe.org/6q6v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6q6v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6q6v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6q6v ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6q6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q6v OCA], [https://pdbe.org/6q6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6q6v RCSB], [https://www.ebi.ac.uk/pdbsum/6q6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6q6v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TRXH_CHLRE TRXH_CHLRE]] Participates in various redox reactions through the reversible oxidation of the active center dithiol to a disulfide. The H form is known to activate a number of cytosolic enzymes.	+	[https://www.uniprot.org/uniprot/TRXH_CHLRE TRXH_CHLRE] Participates in various redox reactions through the reversible oxidation of the active center dithiol to a disulfide. The H form is known to activate a number of cytosolic enzymes.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6q6v" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6q6v" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Thioredoxin 3D structures\|Thioredoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Fermani, S~~]]	+	[[Category: Chlamydomonas reinhardtii]]
-	[[Category: ~~Lemaire, S D~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Zaffagnini, M~~]]	+	[[Category: Fermani S]]
-	[[Category: ~~Alpha/beta protein~~]]	+	[[Category: Lemaire SD]]
-	[[Category: ~~Cell redox homeostati]]~~	+	[[Category: Zaffagnini M]]
-	~~[[Category: Disulphide oxidoreductase]]~~	+
-	~~[[Category: Electron transport]]~~	+
-	~~[[Category: Thioredoxin fold~~]]	+

OCA at 12:17, 16 January 2019

OCA — Wed, 16 Jan 2019 12:17:19 GMT

←Older revision		Revision as of 12:17, 16 January 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6q6v is ~~ON HOLD~~	+	==Crystal structure (trigonal form) of C36S mutant of thioredoxin h1 from Chlamydomonas reinhardtii==
		+	<StructureSection load='6q6v' size='340' side='right' caption='[[6q6v]], [[Resolution\|resolution]] 1.22Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6q6v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q6V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6Q6V FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6q6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q6v OCA], [http://pdbe.org/6q6v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6q6v RCSB], [http://www.ebi.ac.uk/pdbsum/6q6v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6q6v ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TRXH_CHLRE TRXH_CHLRE]] Participates in various redox reactions through the reversible oxidation of the active center dithiol to a disulfide. The H form is known to activate a number of cytosolic enzymes.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Thioredoxins (TRXs) are major protein disulfide reductases of the cell. Their redox activity relies on a conserved Trp-Cys-(Gly/Pro)-Pro-Cys active site bearing two cysteine (Cys) residues that can be found either as free thiols (reduced TRXs) or linked together by a disulfide bond (oxidized TRXs) during the catalytic cycle. Their reactivity is crucial for TRX activity, and depends on the active site microenvironment. Here, we solved and compared the 3D structure of reduced and oxidized TRX h1 from Chlamydomonas reinhardtii (CrTRXh1). The three-dimensional structure was also determined for mutants of each active site Cys. Structural alignments of CrTRXh1 with other structurally solved plant TRXs showed a common spatial fold, despite the low sequence identity. Structural analyses of CrTRXh1 revealed that the protein adopts an identical conformation independently from its redox state. Treatment with iodoacetamide (IAM), a Cys alkylating agent, resulted in a rapid and pH-dependent inactivation of CrTRXh1. Starting from fully reduced CrTRXh1, we determined the acid dissociation constant (pKa) of each active site Cys by Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analyses coupled to differential IAM-based alkylation. Based on the diversity of catalytic Cys deprotonation states, the mechanisms and structural features underlying disulfide redox activity are discussed.

-	~~Authors: Fermani~~, S., ~~Zaffagnini~~, M., Lemaire, S.D.	+	Structural and Biochemical Insights into the Reactivity of Thioredoxin h1 from Chlamydomonas reinhardtii.,Marchand CH, Fermani S, Rossi J, Gurrieri L, Tedesco D, Henri J, Sparla F, Trost P, Lemaire SD, Zaffagnini M Antioxidants (Basel). 2019 Jan 1;8(1). pii: antiox8010010. doi:, 10.3390/antiox8010010. PMID:30609656<ref>PMID:30609656</ref>

-	~~Description: Crystal structure (trigonal form)~~ of ~~C36S mutant~~ of ~~thioredoxin h1 from Chlamydomonas reinhardtii~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 6q6v" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Fermani, S]]		[[Category: Fermani, S]]
		+	[[Category: Lemaire, S D]]
	[[Category: Zaffagnini, M]]		[[Category: Zaffagnini, M]]
-	[[Category: ~~Lemaire, S.D~~]]	+	[[Category: Alpha/beta protein]]
		+	[[Category: Cell redox homeostati]]
		+	[[Category: Disulphide oxidoreductase]]
		+	[[Category: Electron transport]]
		+	[[Category: Thioredoxin fold]]

OCA: Protected "6q6v" [edit=sysop:move=sysop]

OCA — Wed, 19 Dec 2018 06:48:00 GMT

Protected "6q6v" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:48, 19 December 2018

OCA: New page: '''Unreleased structure''' The entry 6q6v is ON HOLD Authors: Fermani, S., Zaffagnini, M., Lemaire, S.D. Description: Crystal structure (trigonal form) of C36S mutant of thioredoxin h1...

OCA — Wed, 19 Dec 2018 06:47:59 GMT

New page: '''Unreleased structure''' The entry 6q6v is ON HOLD Authors: Fermani, S., Zaffagnini, M., Lemaire, S.D. Description: Crystal structure (trigonal form) of C36S mutant of thioredoxin h1...

New page

'''Unreleased structure'''

The entry 6q6v is ON HOLD

Authors: Fermani, S., Zaffagnini, M., Lemaire, S.D.

Description: Crystal structure (trigonal form) of C36S mutant of thioredoxin h1 from Chlamydomonas reinhardtii
[[Category: Unreleased Structures]]
[[Category: Fermani, S]]
[[Category: Zaffagnini, M]]
[[Category: Lemaire, S.D]]