6q6z - Revision history

OCA at 11:54, 24 January 2024

OCA — Wed, 24 Jan 2024 11:54:26 GMT

←Older revision		Revision as of 11:54, 24 January 2024
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	<StructureSection load='6q6z' size='340' side='right'caption='[[6q6z]], [[Resolution\|resolution]] 3.48Å' scene=''>		<StructureSection load='6q6z' size='340' side='right'caption='[[6q6z]], [[Resolution\|resolution]] 3.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6q6z]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~] and [~~http~~://en.wikipedia.org/wiki/~~Camelus_glama Camelus~~ glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q6Z OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6Q6Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6q6z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q6Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Q6Z FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[6i8h\|6i8h]], [[6i8g\|6i8g]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.476Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">EDS1 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=3702 ARATH])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6q6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q6z OCA], [https://pdbe.org/6q6z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6q6z RCSB], [https://www.ebi.ac.uk/pdbsum/6q6z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6q6z ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6q6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q6z OCA], [~~http~~://pdbe.org/6q6z PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6q6z RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6q6z PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6q6z ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/EDS1L_ARATH EDS1L_ARATH]] Positive regulator of basal resistance and of effector-triggered immunity specifically mediated by TIR-NB-LRR resistance proteins. Disruption by bacterial effector of EDS1-TIR-NB-LRR resistance protein interactions constitutes the first step in resistance activation (PubMed:22158819). Triggers early plant defenses and hypersensitive response independently of PAD4, and then recruits PAD4 to potentiate plant defenses through the accumulation of salicylic acid (PubMed:11574472). Nuclear localization is essential for basal and TIR-NB-LRR-conditioned immunity and for reprogramming defense gene expression, while cytoplasmic EDS1 is required to induce a complete immune response (PubMed:20617163). Heterodimerization with PAD4 or SGA101 is necessary for TNL-mediated effector-triggered immunity (PubMed:24331460). Contributes to nonhost resistance against E.amylovora (PubMed:22316300). Has no direct lipase activity (PubMed:16040633).<ref>PMID:10077677</ref> <ref>PMID:11574472</ref> <ref>PMID:20617163</ref> <ref>PMID:22158819</ref> <ref>PMID:22316300</ref> <ref>PMID:24331460</ref> <ref>PMID:16040633</ref>	+	[https://www.uniprot.org/uniprot/EDS1L_ARATH EDS1L_ARATH] Positive regulator of basal resistance and of effector-triggered immunity specifically mediated by TIR-NB-LRR resistance proteins. Disruption by bacterial effector of EDS1-TIR-NB-LRR resistance protein interactions constitutes the first step in resistance activation (PubMed:22158819). Triggers early plant defenses and hypersensitive response independently of PAD4, and then recruits PAD4 to potentiate plant defenses through the accumulation of salicylic acid (PubMed:11574472). Nuclear localization is essential for basal and TIR-NB-LRR-conditioned immunity and for reprogramming defense gene expression, while cytoplasmic EDS1 is required to induce a complete immune response (PubMed:20617163). Heterodimerization with PAD4 or SGA101 is necessary for TNL-mediated effector-triggered immunity (PubMed:24331460). Contributes to nonhost resistance against E.amylovora (PubMed:22316300). Has no direct lipase activity (PubMed:16040633).<ref>PMID:10077677</ref> <ref>PMID:11574472</ref> <ref>PMID:20617163</ref> <ref>PMID:22158819</ref> <ref>PMID:22316300</ref> <ref>PMID:24331460</ref> <ref>PMID:16040633</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
-	[[Category: ~~Camelus~~ glama]]	+	[[Category: Lama glama]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Niefind, K]]	+	[[Category: Niefind K]]
-	[[Category: Toelzer, C]]	+	[[Category: Toelzer C]]
-	[[Category: Voss, M]]	+	[[Category: Voss M]]
-	~~[[Category: Alpha/beta hydrolase fold]]~~	+
-	~~[[Category: Enhanced disease susceptibility 1]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Nanobody]]~~	+
-	~~[[Category: Plant innate immune system~~]]	+

OCA at 11:48, 13 November 2019

OCA — Wed, 13 Nov 2019 11:48:49 GMT

←Older revision		Revision as of 11:48, 13 November 2019
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	<StructureSection load='6q6z' size='340' side='right'caption='[[6q6z]], [[Resolution\|resolution]] 3.48Å' scene=''>		<StructureSection load='6q6z' size='340' side='right'caption='[[6q6z]], [[Resolution\|resolution]] 3.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6q6z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q6Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6Q6Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6q6z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath] and [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q6Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6Q6Z FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6i8h\|6i8h]], [[6i8g\|6i8g]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6i8h\|6i8h]], [[6i8g\|6i8g]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EDS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6q6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q6z OCA], [http://pdbe.org/6q6z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6q6z RCSB], [http://www.ebi.ac.uk/pdbsum/6q6z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6q6z ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6q6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q6z OCA], [http://pdbe.org/6q6z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6q6z RCSB], [http://www.ebi.ac.uk/pdbsum/6q6z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6q6z ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
		+	[[Category: Camelus glama]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Niefind, K]]		[[Category: Niefind, K]]

OCA at 10:35, 2 October 2019

OCA — Wed, 02 Oct 2019 10:35:43 GMT

←Older revision		Revision as of 10:35, 2 October 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6q6z is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Structure of the plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody ENB21==
		+	<StructureSection load='6q6z' size='340' side='right'caption='[[6q6z]], [[Resolution\|resolution]] 3.48Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6q6z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q6Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6Q6Z FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6i8h\|6i8h]], [[6i8g\|6i8g]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6q6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q6z OCA], [http://pdbe.org/6q6z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6q6z RCSB], [http://www.ebi.ac.uk/pdbsum/6q6z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6q6z ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/EDS1L_ARATH EDS1L_ARATH]] Positive regulator of basal resistance and of effector-triggered immunity specifically mediated by TIR-NB-LRR resistance proteins. Disruption by bacterial effector of EDS1-TIR-NB-LRR resistance protein interactions constitutes the first step in resistance activation (PubMed:22158819). Triggers early plant defenses and hypersensitive response independently of PAD4, and then recruits PAD4 to potentiate plant defenses through the accumulation of salicylic acid (PubMed:11574472). Nuclear localization is essential for basal and TIR-NB-LRR-conditioned immunity and for reprogramming defense gene expression, while cytoplasmic EDS1 is required to induce a complete immune response (PubMed:20617163). Heterodimerization with PAD4 or SGA101 is necessary for TNL-mediated effector-triggered immunity (PubMed:24331460). Contributes to nonhost resistance against E.amylovora (PubMed:22316300). Has no direct lipase activity (PubMed:16040633).<ref>PMID:10077677</ref> <ref>PMID:11574472</ref> <ref>PMID:20617163</ref> <ref>PMID:22158819</ref> <ref>PMID:22316300</ref> <ref>PMID:24331460</ref> <ref>PMID:16040633</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In plant innate immunity, enhanced disease susceptibility 1 (EDS1) integrates all pathogen-induced signals transmitted by TIR-type NLR receptors. Driven by an N-terminal alpha/beta-hydrolase-folded domain with a protruding interaction helix, EDS1 assembles with two homologs, phytoalexin-deficient 4 (PAD4) and senescence-associated gene 101 (SAG101). The resulting heterodimers are critical for EDS1 function and structurally well characterized. Here, we resolve solution and crystal structures of unbound Arabidopsis thaliana EDS1 (AtEDS1) using nanobodies for crystallization. These structures, together with gel filtration and immunoprecipitation data, show that PAD4/SAG101-unbound AtEDS1 is stable as a monomer and does not form the homodimers recorded in public databases. Its PAD4/SAG101 anchoring helix is disordered unless engaged in protein/protein interactions. As in the complex with SAG101, monomeric AtEDS1 has a substrate-inaccessible esterase triad with a blocked oxyanion hole and without space for a covalent acyl intermediate. These new structures suggest that the AtEDS1 monomer represents an inactive or pre-activated ground state.

-	~~Authors: Niefind, K~~., Voss, M., Toelzer, C.	+	Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.,Voss M, Toelzer C, Bhandari DD, Parker JE, Niefind K J Struct Biol. 2019 Sep 21. pii: S1047-8477(19)30201-1. doi:, 10.1016/j.jsb.2019.09.007. PMID:31550533<ref>PMID:31550533</ref>

-	~~Description: Structure~~ of the ~~plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody ENB21~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Voss, M]]~~	+	<div class="pdbe-citations 6q6z" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Toelzer, C~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Niefind, K]]		[[Category: Niefind, K]]
		+	[[Category: Toelzer, C]]
		+	[[Category: Voss, M]]
		+	[[Category: Alpha/beta hydrolase fold]]
		+	[[Category: Enhanced disease susceptibility 1]]
		+	[[Category: Immune system]]
		+	[[Category: Nanobody]]
		+	[[Category: Plant innate immune system]]

OCA at 04:18, 17 April 2019

OCA — Wed, 17 Apr 2019 04:18:57 GMT

←Older revision		Revision as of 04:18, 17 April 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6q6z is ON HOLD	+	The entry 6q6z is ON HOLD until Paper Publication

	Authors: Niefind, K., Voss, M., Toelzer, C.		Authors: Niefind, K., Voss, M., Toelzer, C.

-	Description: Structure of the plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody ~~1AT21~~	+	Description: Structure of the plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody ENB21
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Voss, M]]		[[Category: Voss, M]]
	[[Category: Toelzer, C]]		[[Category: Toelzer, C]]
	[[Category: Niefind, K]]		[[Category: Niefind, K]]

OCA: Protected "6q6z" [edit=sysop:move=sysop]

OCA — Wed, 19 Dec 2018 06:48:23 GMT

Protected "6q6z" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:48, 19 December 2018

OCA: New page: '''Unreleased structure''' The entry 6q6z is ON HOLD Authors: Niefind, K., Voss, M., Toelzer, C. Description: Structure of the plant immune signaling node EDS1 (enhanced disease suscep...

OCA — Wed, 19 Dec 2018 06:48:22 GMT

New page: '''Unreleased structure''' The entry 6q6z is ON HOLD Authors: Niefind, K., Voss, M., Toelzer, C. Description: Structure of the plant immune signaling node EDS1 (enhanced disease suscep...

New page

'''Unreleased structure'''

The entry 6q6z is ON HOLD

Authors: Niefind, K., Voss, M., Toelzer, C.

Description: Structure of the plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody 1AT21
[[Category: Unreleased Structures]]
[[Category: Voss, M]]
[[Category: Toelzer, C]]
[[Category: Niefind, K]]