OCA at 07:03, 26 June 2019

OCA — Wed, 26 Jun 2019 07:03:09 GMT

←Older revision		Revision as of 07:03, 26 June 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6q9j is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of reduced Aquifex aeolicus NADH-quinone oxidoreductase subunits NuoE G129S and NuoF bound to NADH==
		+	<StructureSection load='6q9j' size='340' side='right'caption='[[6q9j]], [[Resolution\|resolution]] 1.83Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6q9j]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q9J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6Q9J FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6q9g\|6q9g]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADH_dehydrogenase_(quinone) NADH dehydrogenase (quinone)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.5.11 1.6.5.11] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6q9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q9j OCA], [http://pdbe.org/6q9j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6q9j RCSB], [http://www.ebi.ac.uk/pdbsum/6q9j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6q9j ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NUOE_AQUAE NUOE_AQUAE]] NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity). [[http://www.uniprot.org/uniprot/NUOF_AQUAE NUOF_AQUAE]] NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Respiratory complex I plays a central role in cellular energy metabolism coupling NADH oxidation to proton translocation. In humans its dysfunction is associated with degenerative diseases. Here we report the structure of the electron input part of Aquifex aeolicus complex I at up to 1.8 A resolution with bound substrates in the reduced and oxidized states. The redox states differ by the flip of a peptide bond close to the NADH binding site. The orientation of this peptide bond is determined by the reduction state of the nearby [Fe-S] cluster N1a. Fixation of the peptide bond by site-directed mutagenesis led to an inactivation of electron transfer and a decreased reactive oxygen species (ROS) production. We suggest the redox-gated peptide flip to represent a previously unrecognized molecular switch synchronizing NADH oxidation in response to the redox state of the complex as part of an intramolecular feed-back mechanism to prevent ROS production.

-	~~Authors: Wohlwend, D~~., ~~Gerhardt~~, S., Gnandt, E., ~~Friedrich~~, T.	+	A mechanism to prevent production of reactive oxygen species by Escherichia coli respiratory complex I.,Schulte M, Frick K, Gnandt E, Jurkovic S, Burschel S, Labatzke R, Aierstock K, Fiegen D, Wohlwend D, Gerhardt S, Einsle O, Friedrich T Nat Commun. 2019 Jun 11;10(1):2551. doi: 10.1038/s41467-019-10429-0. PMID:31186428<ref>PMID:31186428</ref>

-	~~Description: Crystal structure~~ of ~~reduced Aquifex aeolicus NADH~~-~~quinone oxidoreductase subunits NuoE G129S and NuoF bound to NADH~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6q9j" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Friedrich, T]]
		+	[[Category: Gerhardt, S]]
	[[Category: Gnandt, E]]		[[Category: Gnandt, E]]
	[[Category: Wohlwend, D]]		[[Category: Wohlwend, D]]
-	[[Category: ~~Gerhardt, S~~]]	+	[[Category: Complex i]]
-	[[Category: ~~Friedrich, T~~]]	+	[[Category: Fe-s cluster]]
		+	[[Category: Nadh ubiquinone oxidoreductase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Respiratory chain]]

OCA at 08:15, 10 May 2019

OCA — Fri, 10 May 2019 08:15:19 GMT

←Older revision		Revision as of 08:15, 10 May 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6q9j is ON HOLD	+	The entry 6q9j is ON HOLD until Paper Publication

	Authors: Wohlwend, D., Gerhardt, S., Gnandt, E., Friedrich, T.		Authors: Wohlwend, D., Gerhardt, S., Gnandt, E., Friedrich, T.
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	Description: Crystal structure of reduced Aquifex aeolicus NADH-quinone oxidoreductase subunits NuoE G129S and NuoF bound to NADH		Description: Crystal structure of reduced Aquifex aeolicus NADH-quinone oxidoreductase subunits NuoE G129S and NuoF bound to NADH
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Gerhardt, S]]
	[[Category: Gnandt, E]]		[[Category: Gnandt, E]]
	[[Category: Wohlwend, D]]		[[Category: Wohlwend, D]]
		+	[[Category: Gerhardt, S]]
	[[Category: Friedrich, T]]		[[Category: Friedrich, T]]

OCA: Protected "6q9j" [edit=sysop:move=sysop]

OCA — Wed, 26 Dec 2018 07:00:32 GMT

Protected "6q9j" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:00, 26 December 2018

OCA: New page: '''Unreleased structure''' The entry 6q9j is ON HOLD Authors: Wohlwend, D., Gerhardt, S., Gnandt, E., Friedrich, T. Description: Crystal structure of reduced Aquifex aeolicus NADH-quin...

OCA — Wed, 26 Dec 2018 07:00:30 GMT

New page: '''Unreleased structure''' The entry 6q9j is ON HOLD Authors: Wohlwend, D., Gerhardt, S., Gnandt, E., Friedrich, T. Description: Crystal structure of reduced Aquifex aeolicus NADH-quin...

New page

'''Unreleased structure'''

The entry 6q9j is ON HOLD

Authors: Wohlwend, D., Gerhardt, S., Gnandt, E., Friedrich, T.

Description: Crystal structure of reduced Aquifex aeolicus NADH-quinone oxidoreductase subunits NuoE G129S and NuoF bound to NADH
[[Category: Unreleased Structures]]
[[Category: Gerhardt, S]]
[[Category: Gnandt, E]]
[[Category: Wohlwend, D]]
[[Category: Friedrich, T]]

6q9j - Revision history

OCA at 07:03, 26 June 2019

OCA at 08:15, 10 May 2019

OCA: Protected "6q9j" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6q9j is ON HOLD Authors: Wohlwend, D., Gerhardt, S., Gnandt, E., Friedrich, T. Description: Crystal structure of reduced Aquifex aeolicus NADH-quin...