6qel - Revision history

OCA at 04:57, 11 April 2020

OCA — Sat, 11 Apr 2020 04:57:25 GMT

←Older revision		Revision as of 04:57, 11 April 2020
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	<SX load='6qel' size='340' side='right' viewer='molstar' caption='[[6qel]], [[Resolution\|resolution]] 3.90Å' scene=''>		<SX load='6qel' size='340' side='right' viewer='molstar' caption='[[6qel]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qel]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QEL OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QEL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qel]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QEL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QEL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=08T:[[[(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-BIS(OXIDANYL)OXOLAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]OXY-OXIDANYL-PHOSPHORYL]OXY-TRIS(FLUORANYL)BERYLLIUM'>08T</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=08T:[[[(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-BIS(OXIDANYL)OXOLAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]OXY-OXIDANYL-PHOSPHORYL]OXY-TRIS(FLUORANYL)BERYLLIUM'>08T</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A1UM_04839 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), A1UM_00191 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A1UM_04839 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), A1UM_00191 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qel OCA], [http://pdbe.org/6qel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qel RCSB], [http://www.ebi.ac.uk/pdbsum/6qel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qel ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qel OCA], [http://pdbe.org/6qel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qel RCSB], [http://www.ebi.ac.uk/pdbsum/6qel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qel ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 23:49, 6 March 2020

OCA — Fri, 06 Mar 2020 23:49:48 GMT

←Older revision		Revision as of 23:49, 6 March 2020
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	==E. coli DnaBC apo complex==		==E. coli DnaBC apo complex==
-	<~~StructureSection~~ load='6qel' size='340' side='right'caption='[[6qel]], [[Resolution\|resolution]] 3.90Å' scene=''>	+	<SX load='6qel' size='340' side='right' viewer='molstar' caption='[[6qel]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6qel]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QEL FirstGlance]. <br>		<table><tr><td colspan='2'>[[6qel]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QEL FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: DNA helicase]]		[[Category: DNA helicase]]

OCA at 08:38, 6 November 2019

OCA — Wed, 06 Nov 2019 08:38:23 GMT

←Older revision		Revision as of 08:38, 6 November 2019
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 6qel" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6qel" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Helicase 3D structures\|Helicase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:06, 17 April 2019

OCA — Wed, 17 Apr 2019 07:06:16 GMT

←Older revision		Revision as of 07:06, 17 April 2019
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	==E. coli DnaBC apo complex==		==E. coli DnaBC apo complex==
-	<StructureSection load='6qel' size='340' side='right' caption='[[6qel]], [[Resolution\|resolution]] 3.90Å' scene=''>	+	<StructureSection load='6qel' size='340' side='right'caption='[[6qel]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qel]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QEL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qel]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QEL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=08T:[[[(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-BIS(OXIDANYL)OXOLAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]OXY-OXIDANYL-PHOSPHORYL]OXY-TRIS(FLUORANYL)BERYLLIUM'>08T</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=08T:[[[(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-BIS(OXIDANYL)OXOLAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]OXY-OXIDANYL-PHOSPHORYL]OXY-TRIS(FLUORANYL)BERYLLIUM'>08T</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A1UM_04839 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), A1UM_00191 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qel OCA], [http://pdbe.org/6qel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qel RCSB], [http://www.ebi.ac.uk/pdbsum/6qel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qel ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qel OCA], [http://pdbe.org/6qel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qel RCSB], [http://www.ebi.ac.uk/pdbsum/6qel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qel ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: DNA helicase]]		[[Category: DNA helicase]]
		+	[[Category: Large Structures]]
	[[Category: Arias-Palomo, E]]		[[Category: Arias-Palomo, E]]
	[[Category: Berger, J M]]		[[Category: Berger, J M]]

OCA at 07:37, 6 March 2019

OCA — Wed, 06 Mar 2019 07:37:56 GMT

←Older revision		Revision as of 07:37, 6 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6qel is ~~ON HOLD~~	+	==E. coli DnaBC apo complex==
		+	<StructureSection load='6qel' size='340' side='right' caption='[[6qel]], [[Resolution\|resolution]] 3.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6qel]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QEL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=08T:[[[(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-BIS(OXIDANYL)OXOLAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]OXY-OXIDANYL-PHOSPHORYL]OXY-TRIS(FLUORANYL)BERYLLIUM'>08T</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qel OCA], [http://pdbe.org/6qel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qel RCSB], [http://www.ebi.ac.uk/pdbsum/6qel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qel ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/E3PC72_ECOH1 E3PC72_ECOH1]] Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.[RuleBase:RU362085]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In cells, dedicated AAA+ ATPases deposit hexameric, ring-shaped helicases onto DNA to initiate chromosomal replication. To better understand the mechanisms by which helicase loading can occur, we used cryo-EM to determine sub-4-A-resolution structures of the E. coli DnaBDnaC helicaseloader complex with nucleotide in pre- and post-DNA engagement states. In the absence of DNA, six DnaC protomers latch onto and crack open a DnaB hexamer using an extended N-terminal domain, stabilizing this conformation through nucleotide-dependent ATPase interactions. Upon binding DNA, DnaC hydrolyzes ATP, allowing DnaB to isomerize into a topologically closed, pre-translocation state competent to bind primase. Our data show how DnaC opens the DnaB ring and represses the helicase prior to DNA binding and how DnaC ATPase activity is reciprocally regulated by DnaB and DNA. Comparative analyses reveal how the helicase loading mechanism of DnaC parallels and diverges from homologous AAA+ systems involved in DNA replication and transposition.

-	~~Authors~~:	+	Physical Basis for the Loading of a Bacterial Replicative Helicase onto DNA.,Arias-Palomo E, Puri N, O'Shea Murray VL, Yan Q, Berger JM Mol Cell. 2019 Feb 16. pii: S1097-2765(19)30043-7. doi:, 10.1016/j.molcel.2019.01.023. PMID:30797687<ref>PMID:30797687</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6qel" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: DNA helicase]]
		+	[[Category: Arias-Palomo, E]]
		+	[[Category: Berger, J M]]
		+	[[Category: Murray, V L.O Shea]]
		+	[[Category: Puri, N]]
		+	[[Category: Yan, Q]]
		+	[[Category: Aaa+]]
		+	[[Category: Helicase]]
		+	[[Category: Helicase loader]]
		+	[[Category: Reca]]
		+	[[Category: Replication]]

OCA at 06:52, 30 January 2019

OCA — Wed, 30 Jan 2019 06:52:34 GMT

←Older revision		Revision as of 06:52, 30 January 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6qel is ON HOLD ~~until sometime in the future~~	+	The entry 6qel is ON HOLD

	Authors:		Authors:

OCA: Protected "6qel" [edit=sysop:move=sysop]

OCA — Wed, 16 Jan 2019 10:48:58 GMT

Protected "6qel" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:48, 16 January 2019

OCA: New page: '''Unreleased structure''' The entry 6qel is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

OCA — Wed, 16 Jan 2019 10:48:57 GMT

New page: '''Unreleased structure''' The entry 6qel is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6qel is ON HOLD until sometime in the future

Authors:

Description:
[[Category: Unreleased Structures]]