OCA at 23:12, 5 June 2019

OCA — Wed, 05 Jun 2019 23:12:57 GMT

←Older revision		Revision as of 23:12, 5 June 2019
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	<StructureSection load='6qeq' size='340' side='right'caption='[[6qeq]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6qeq' size='340' side='right'caption='[[6qeq]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qeq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QEQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qeq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QEQ FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pcfF, DAI13_16755 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1351 "Enterococcus proteiformis" Thiercelin and Jouhaud 1903])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qeq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qeq OCA], [http://pdbe.org/6qeq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qeq RCSB], [http://www.ebi.ac.uk/pdbsum/6qeq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qeq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qeq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qeq OCA], [http://pdbe.org/6qeq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qeq RCSB], [http://www.ebi.ac.uk/pdbsum/6qeq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qeq ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The conjugative plasmid pCF10 from Enterococcus faecalis encodes a Type 4 Secretion System required for plasmid transfer. The accessory factor PcfF and relaxase PcfG initiate pCF10 transfer by forming the catalytically active relaxosome at the plasmid's origin-of-transfer (oriT) sequence. Here, we report the crystal structure of the homo-dimeric PcfF, composed of an N-terminal DNA binding Ribbon-Helix-Helix (RHH) domain and a C-terminal stalk domain. We identified key residues in the RHH domain that are responsible for binding pCF10's oriT sequence in vitro, and further showed that PcfF bends the DNA upon oriT binding. By mutational analysis and pull-down experiments, we identified residues in the stalk domain that contribute to interaction with PcfG. PcfF variant proteins defective in oriT or PcfG binding attenuated plasmid transfer in vivo, but also suggested that intrinsic or extrinsic factors might modulate relaxosome assembly. We propose that PcfF initiates relaxosome assembly by binding oriT and inducing DNA bending, which serves to recruit PcfG as well as extrinsic factors necessary for optimal plasmid processing and engagement with the pCF10 transfer machine.
		+
		+	Enterococcal PcfF Is a Ribbon-Helix-Helix Protein That Recruits the Relaxase PcfG Through Binding and Bending of the oriT Sequence.,Rehman S, Li YG, Schmitt A, Lassinantti L, Christie PJ, Berntsson RP Front Microbiol. 2019 May 7;10:958. doi: 10.3389/fmicb.2019.00958. eCollection, 2019. PMID:31134011<ref>PMID:31134011</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6qeq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Berntsson, R P.A]]		[[Category: Berntsson, R P.A]]

OCA at 08:57, 1 May 2019

OCA — Wed, 01 May 2019 08:57:57 GMT

←Older revision		Revision as of 08:57, 1 May 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6qeq ~~is ON HOLD until Jan 08 2021~~	+	==PcfF from Enterococcus faecalis pCF10==
-		+	<StructureSection load='6qeq' size='340' side='right'caption='[[6qeq]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6qeq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QEQ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qeq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qeq OCA], [http://pdbe.org/6qeq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qeq RCSB], [http://www.ebi.ac.uk/pdbsum/6qeq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qeq ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Berntsson, R P.A]]
		+	[[Category: Rehman, S]]
		+	[[Category: Accessory factor]]
		+	[[Category: Dna binding protein]]

OCA: Protected "6qeq" [edit=sysop:move=sysop]

OCA — Wed, 16 Jan 2019 10:49:10 GMT

Protected "6qeq" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:49, 16 January 2019

OCA: New page: '''Unreleased structure''' The entry 6qeq is ON HOLD until Jan 08 2021 Authors: Description: Category: Unreleased Structures

OCA — Wed, 16 Jan 2019 10:49:09 GMT

New page: '''Unreleased structure''' The entry 6qeq is ON HOLD until Jan 08 2021 Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6qeq is ON HOLD until Jan 08 2021

Authors:

Description:
[[Category: Unreleased Structures]]

6qeq - Revision history

OCA at 23:12, 5 June 2019

OCA at 08:57, 1 May 2019

OCA: Protected "6qeq" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6qeq is ON HOLD until Jan 08 2021 Authors: Description: Category: Unreleased Structures