6qfk - Revision history

OCA at 11:59, 24 January 2024

OCA — Wed, 24 Jan 2024 11:59:22 GMT

←Older revision		Revision as of 11:59, 24 January 2024
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	<StructureSection load='6qfk' size='340' side='right'caption='[[6qfk]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6qfk' size='340' side='right'caption='[[6qfk]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qfk]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_1~~.~~2186 As 1~~.~~2186~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QFK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6QFK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qfk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bifidobacterium_longum Bifidobacterium longum] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QFK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qev\|4qev]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qfk OCA], [https://pdbe.org/6qfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qfk RCSB], [https://www.ebi.ac.uk/pdbsum/6qfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qfk ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DW792_04840 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216816 AS 1.2186])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6qfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qfk OCA], [~~http~~://pdbe.org/6qfk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6qfk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6qfk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6qfk ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~As 1 2186~~]]	+	[[Category: Bifidobacterium longum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ernst, P~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Mittl,~~ P ~~R.E~~]]	+	[[Category: Ernst P]]
-	[[Category: ~~Pluckthun, A~~]]	+	[[Category: Mittl PRE]]
-	[[Category: ~~Chaperone]]~~	+	[[Category: Pluckthun A]]
-	~~[[Category: Crystallization chaperone]]~~	+
-	~~[[Category: Darpin]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protein fusion~~]]	+

OCA at 06:04, 22 April 2020

OCA — Wed, 22 Apr 2020 06:04:25 GMT

←Older revision		Revision as of 06:04, 22 April 2020
Line 3:		Line 3:
	<StructureSection load='6qfk' size='340' side='right'caption='[[6qfk]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6qfk' size='340' side='right'caption='[[6qfk]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qfk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QFK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QFK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qfk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_1.2186 As 1.2186]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QFK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QFK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qev\|4qev]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qev\|4qev]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qfk OCA], [http://pdbe.org/6qfk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qfk RCSB], [http://www.ebi.ac.uk/pdbsum/6qfk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qfk ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DW792_04840 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216816 AS 1.2186])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qfk OCA], [http://pdbe.org/6qfk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qfk RCSB], [http://www.ebi.ac.uk/pdbsum/6qfk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qfk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: As 1 2186]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ernst, P]]		[[Category: Ernst, P]]

OCA at 06:48, 6 November 2019

OCA — Wed, 06 Nov 2019 06:48:20 GMT

←Older revision		Revision as of 06:48, 6 November 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6qfk is ~~ON HOLD until Paper~~ Publication	+	==EngBF DARPin Fusion 4b G10==
		+	<StructureSection load='6qfk' size='340' side='right'caption='[[6qfk]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6qfk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QFK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QFK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qev\|4qev]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qfk OCA], [http://pdbe.org/6qfk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qfk RCSB], [http://www.ebi.ac.uk/pdbsum/6qfk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qfk ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	To overcome the laborious identification of crystallisation conditions for protein X-ray crystallography, we developed a method where the examined protein is immobilised as a guest molecule in a universal host lattice. We applied crystal engineering to create a generic crystalline host lattice under reproducible, predefined conditions and analysed the structures of target guest molecules of different size, namely two 15-mer peptides and green fluorescent protein (sfGFP). A fusion protein with an N-terminal endo-alpha-N-acetylgalactosaminidase (EngBF) domain and a C-terminal designed ankyrin repeat protein (DARPin) domain establishes the crystal lattice. The target is recruited into the host lattice, always in the same crystal form, through binding to the DARPin. The target structures can be determined rapidly from difference Fourier maps, whose quality depends on the size of the target and the orientation of the DARPin.

-	~~Authors~~: ~~Ernst~~, P., Pluckthun, A., Mittl~~, P~~.R.E.	+	Structural analysis of biological targets by host:guest crystal lattice engineering.,Ernst P, Pluckthun A, Mittl PRE Sci Rep. 2019 Oct 23;9(1):15199. doi: 10.1038/s41598-019-51017-y. PMID:31645583<ref>PMID:31645583</ref>

-	~~Description: EngBF DARPin Fusion 4b G10~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Pluckthun, A]]~~	+	<div class="pdbe-citations 6qfk" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Mittl, P.R.E~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ernst, P]]		[[Category: Ernst, P]]
		+	[[Category: Mittl, P R.E]]
		+	[[Category: Pluckthun, A]]
		+	[[Category: Chaperone]]
		+	[[Category: Crystallization chaperone]]
		+	[[Category: Darpin]]
		+	[[Category: Hydrolase]]
		+	[[Category: Protein fusion]]

OCA at 04:52, 31 July 2019

OCA — Wed, 31 Jul 2019 04:52:43 GMT

←Older revision		Revision as of 04:52, 31 July 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6qfk is ON HOLD	+	The entry 6qfk is ON HOLD until Paper Publication

	Authors: Ernst, P., Pluckthun, A., Mittl, P.R.E.		Authors: Ernst, P., Pluckthun, A., Mittl, P.R.E.

OCA: Protected "6qfk" [edit=sysop:move=sysop]

OCA — Wed, 16 Jan 2019 10:52:13 GMT

Protected "6qfk" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:52, 16 January 2019

OCA: New page: '''Unreleased structure''' The entry 6qfk is ON HOLD Authors: Ernst, P., Pluckthun, A., Mittl, P.R.E. Description: EngBF DARPin Fusion 4b G10 Category: Unreleased Structures [[Cate...

OCA — Wed, 16 Jan 2019 10:52:11 GMT

New page: '''Unreleased structure''' The entry 6qfk is ON HOLD Authors: Ernst, P., Pluckthun, A., Mittl, P.R.E. Description: EngBF DARPin Fusion 4b G10 Category: Unreleased Structures [[Cate...

New page

'''Unreleased structure'''

The entry 6qfk is ON HOLD

Authors: Ernst, P., Pluckthun, A., Mittl, P.R.E.

Description: EngBF DARPin Fusion 4b G10
[[Category: Unreleased Structures]]
[[Category: Pluckthun, A]]
[[Category: Mittl, P.R.E]]
[[Category: Ernst, P]]