6qga - Revision history

OCA at 11:59, 24 January 2024

OCA — Wed, 24 Jan 2024 11:59:55 GMT

←Older revision		Revision as of 11:59, 24 January 2024
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	<StructureSection load='6qga' size='340' side='right'caption='[[6qga]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6qga' size='340' side='right'caption='[[6qga]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qga]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Idesa Idesa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QGA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QGA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qga]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Ideonella_sakaiensis Ideonella sakaiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QGA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QGA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=J1K:4-(2-hydroxyethylcarbamoyl)benzoic+acid'>J1K</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ISF6_0224 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1547922 IDESA])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=J1K:4-(2-hydroxyethylcarbamoyl)benzoic+acid'>J1K</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mono(ethylene_terephthalate)_hydrolase Mono(ethylene terephthalate) hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.102 3.1.1.102] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qga OCA], [https://pdbe.org/6qga PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qga RCSB], [https://www.ebi.ac.uk/pdbsum/6qga PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qga ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qga OCA], [~~http~~://pdbe.org/6qga PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6qga RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6qga PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6qga ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MHETH_IDESA MHETH_IDESA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Idesa~~]]	+	[[Category: Ideonella sakaiensis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Berndt, L]]	+	[[Category: Berndt L]]
-	[[Category: Boettcher, D]]	+	[[Category: Boettcher D]]
-	[[Category: Bornscheuer~~, U T~~]]	+	[[Category: Bornscheuer UT]]
-	[[Category: Michels~~, E A.P~~]]	+	[[Category: Michels EAP]]
-	[[Category: Mueller, H]]	+	[[Category: Mueller H]]
-	[[Category: Palm~~, G J~~]]	+	[[Category: Palm GJ]]
-	[[Category: Reisky, L]]	+	[[Category: Reisky L]]
-	[[Category: Walczak, C]]	+	[[Category: Walczak C]]
-	[[Category: Weber, G]]	+	[[Category: Weber G]]
-	[[Category: Weiss~~, M S]]~~	+	[[Category: Weiss MS]]
-	~~[[Category: Alpha/beta hydrolase fold]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: I. sakaiensis catalytic triad]]~~	+
-	~~[[Category: Plastic-degrading hydrolase~~]]	+

OCA at 08:25, 24 April 2019

OCA — Wed, 24 Apr 2019 08:25:43 GMT

←Older revision		Revision as of 08:25, 24 April 2019
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qga OCA], [http://pdbe.org/6qga PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qga RCSB], [http://www.ebi.ac.uk/pdbsum/6qga PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qga ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qga OCA], [http://pdbe.org/6qga PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qga RCSB], [http://www.ebi.ac.uk/pdbsum/6qga PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qga ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The extreme durability of polyethylene terephthalate (PET) debris has rendered it a long-term environmental burden. At the same time, current recycling efforts still lack sustainability. Two recently discovered bacterial enzymes that specifically degrade PET represent a promising solution. First, Ideonella sakaiensis PETase, a structurally well-characterized consensus alpha/beta-hydrolase fold enzyme, converts PET to mono-(2-hydroxyethyl) terephthalate (MHET). MHETase, the second key enzyme, hydrolyzes MHET to the PET educts terephthalate and ethylene glycol. Here, we report the crystal structures of active ligand-free MHETase and MHETase bound to a nonhydrolyzable MHET analog. MHETase, which is reminiscent of feruloyl esterases, possesses a classic alpha/beta-hydrolase domain and a lid domain conferring substrate specificity. In the light of structure-based mapping of the active site, activity assays, mutagenesis studies and a first structure-guided alteration of substrate specificity towards bis-(2-hydroxyethyl) terephthalate (BHET) reported here, we anticipate MHETase to be a valuable resource to further advance enzymatic plastic degradation.
		+
		+	Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate.,Palm GJ, Reisky L, Bottcher D, Muller H, Michels EAP, Walczak MC, Berndt L, Weiss MS, Bornscheuer UT, Weber G Nat Commun. 2019 Apr 12;10(1):1717. doi: 10.1038/s41467-019-09326-3. PMID:30979881<ref>PMID:30979881</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6qga" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:06, 17 April 2019

OCA — Wed, 17 Apr 2019 07:06:49 GMT

←Older revision		Revision as of 07:06, 17 April 2019
Line 1:		Line 1:

-	==Crystal structure of ~~a plastic degrading enzyme in complex with a~~ non-hydrolyzable ~~substrate analog~~==	+	==Crystal structure of Ideonella sakaiensis MHETase bound to the non-hydrolyzable ligand MHETA==
	<StructureSection load='6qga' size='340' side='right'caption='[[6qga]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6qga' size='340' side='right'caption='[[6qga]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qga]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QGA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QGA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qga]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Idesa Idesa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QGA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QGA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=J1K:4-(2-hydroxyethylcarbamoyl)benzoic+acid'>J1K</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=J1K:4-(2-hydroxyethylcarbamoyl)benzoic+acid'>J1K</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ISF6_0224 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1547922 IDESA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mono(ethylene_terephthalate)_hydrolase Mono(ethylene terephthalate) hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.102 3.1.1.102] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mono(ethylene_terephthalate)_hydrolase Mono(ethylene terephthalate) hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.102 3.1.1.102] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qga OCA], [http://pdbe.org/6qga PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qga RCSB], [http://www.ebi.ac.uk/pdbsum/6qga PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qga ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qga OCA], [http://pdbe.org/6qga PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qga RCSB], [http://www.ebi.ac.uk/pdbsum/6qga PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qga ProSAT]</span></td></tr>
Line 10:		Line 11:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Idesa]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Berndt, L]]		[[Category: Berndt, L]]

OCA at 07:13, 3 April 2019

OCA — Wed, 03 Apr 2019 07:13:56 GMT

←Older revision		Revision as of 07:13, 3 April 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6qga is ON HOLD until Paper Publication~~	+	==Crystal structure of a plastic degrading enzyme in complex with a non-hydrolyzable substrate analog==
-		+	<StructureSection load='6qga' size='340' side='right'caption='[[6qga]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~Authors: Palm, G.J., Reisky, L., Boettcher, D., Mueller, H., Michels, E.A.P., Walczak, C., Berndt, L., Weiss, M.S., Bornscheuer, U.T., Weber, G.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6qga]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QGA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QGA FirstGlance]. <br>
-	~~Description:~~ Crystal structure of a plastic degrading enzyme in complex with a non-hydrolyzable substrate analog	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=J1K:4-(2-hydroxyethylcarbamoyl)benzoic+acid'>J1K</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[~~Category: Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mono(ethylene_terephthalate)_hydrolase Mono(ethylene terephthalate) hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.102 3.1.1.102] </span></td></tr>
-	[[~~Category~~: ~~Bornscheuer, U~~.T]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qga OCA], [http://pdbe.org/6qga PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qga RCSB], [http://www.ebi.ac.uk/pdbsum/6qga PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qga ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Weber, G~~]]	+	</table>
-	[~~[Category~~: ~~Mueller~~, H]]	+	__TOC__
-	[[~~Category~~: ~~Michels~~, E.A.P]]	+	</StructureSection>
-	[[Category: ~~Palm, G.J~~]]	+	[[Category: Large Structures]]
	[[Category: Berndt, L]]		[[Category: Berndt, L]]
-	[[Category: Walczak, C]]
	[[Category: Boettcher, D]]		[[Category: Boettcher, D]]
		+	[[Category: Bornscheuer, U T]]
		+	[[Category: Michels, E A.P]]
		+	[[Category: Mueller, H]]
		+	[[Category: Palm, G J]]
	[[Category: Reisky, L]]		[[Category: Reisky, L]]
-	[[Category: Weiss, M.S]]	+	[[Category: Walczak, C]]
		+	[[Category: Weber, G]]
		+	[[Category: Weiss, M S]]
		+	[[Category: Alpha/beta hydrolase fold]]
		+	[[Category: Hydrolase]]
		+	[[Category: I. sakaiensis catalytic triad]]
		+	[[Category: Plastic-degrading hydrolase]]

OCA at 06:54, 30 January 2019

OCA — Wed, 30 Jan 2019 06:54:07 GMT

←Older revision		Revision as of 06:54, 30 January 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6qga is ON HOLD	+	The entry 6qga is ON HOLD until Paper Publication

	Authors: Palm, G.J., Reisky, L., Boettcher, D., Mueller, H., Michels, E.A.P., Walczak, C., Berndt, L., Weiss, M.S., Bornscheuer, U.T., Weber, G.		Authors: Palm, G.J., Reisky, L., Boettcher, D., Mueller, H., Michels, E.A.P., Walczak, C., Berndt, L., Weiss, M.S., Bornscheuer, U.T., Weber, G.

-	Description: Crystal structure of a plastic degrading enzyme in complex with a ~~ligand~~	+	Description: Crystal structure of a plastic degrading enzyme in complex with a non-hydrolyzable substrate analog
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Bornscheuer, U.T]]		[[Category: Bornscheuer, U.T]]

OCA: Protected "6qga" [edit=sysop:move=sysop]

OCA — Wed, 16 Jan 2019 10:53:56 GMT

Protected "6qga" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:53, 16 January 2019

OCA: New page: '''Unreleased structure''' The entry 6qga is ON HOLD Authors: Palm, G.J., Reisky, L., Boettcher, D., Mueller, H., Michels, E.A.P., Walczak, C., Berndt, L., Weiss, M.S., Bornscheuer, U.T...

OCA — Wed, 16 Jan 2019 10:53:55 GMT

New page: '''Unreleased structure''' The entry 6qga is ON HOLD Authors: Palm, G.J., Reisky, L., Boettcher, D., Mueller, H., Michels, E.A.P., Walczak, C., Berndt, L., Weiss, M.S., Bornscheuer, U.T...

New page

'''Unreleased structure'''

The entry 6qga is ON HOLD

Authors: Palm, G.J., Reisky, L., Boettcher, D., Mueller, H., Michels, E.A.P., Walczak, C., Berndt, L., Weiss, M.S., Bornscheuer, U.T., Weber, G.

Description: Crystal structure of a plastic degrading enzyme in complex with a ligand
[[Category: Unreleased Structures]]
[[Category: Bornscheuer, U.T]]
[[Category: Weber, G]]
[[Category: Mueller, H]]
[[Category: Michels, E.A.P]]
[[Category: Palm, G.J]]
[[Category: Berndt, L]]
[[Category: Walczak, C]]
[[Category: Boettcher, D]]
[[Category: Reisky, L]]
[[Category: Weiss, M.S]]