6qj6 - Revision history

OCA at 07:29, 19 February 2020

OCA — Wed, 19 Feb 2020 07:29:16 GMT

←Older revision		Revision as of 07:29, 19 February 2020
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/Q63SB3_BURPS Q63SB3_BURPS]] Removes the phosphate from trehalose 6-phosphate to produce free trehalose.[RuleBase:RU361117]		[[http://www.uniprot.org/uniprot/Q63SB3_BURPS Q63SB3_BURPS]] Removes the phosphate from trehalose 6-phosphate to produce free trehalose.[RuleBase:RU361117]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We report the functional and structural characterization of trehalose-6-phosphate phosphatase (TPP), from the Gram-negative bacterium B. pseudomallei that causes melioidosis, a severe infectious disease endemic in Southeast Asia and Northern Australia. TPP is a key enzyme in the trehalose biosynthesis pathway, which plays an important role in bacterial stress responses. Due to the absence of this biosynthetic pathway in mammals, TPP has drawn attention as a potential drug target, to combat antibiotic resistance. In this context, we present a detailed biochemical analysis of purified recombinant TPP, reporting its specific high catalytic activity toward the trehalose-6-phosphate substrate, and an absolute requirement for its Mg(2+) cofactor. Furthermore, we present the crystal structure of TPP solved at 1.74 A, revealing the canonical haloacid dehalogenase (HAD) superfamily fold and conserved substrate binding pocket, from which insights into the catalytic mechanism may be deduced. Our data represent a starting point for the rational design of antibacterial drugs.
		+
		+	Functional and structural analysis of trehalose-6-phosphate phosphatase from Burkholderia pseudomallei: Insights into the catalytic mechanism.,Suthisawat S, Gourlay LJ, Bolognesi M, Boonyuen U, Vanaporn M Biochem Biophys Res Commun. 2020 Jan 20. pii: S0006-291X(20)30018-8. doi:, 10.1016/j.bbrc.2019.12.088. PMID:31973820<ref>PMID:31973820</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6qj6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:17, 29 January 2020

OCA — Wed, 29 Jan 2020 16:17:04 GMT

←Older revision		Revision as of 16:17, 29 January 2020
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-	'''Unreleased structure'''

-	The ~~entry~~ 6qj6 is ~~ON HOLD until Paper Publication~~	+	==The structure of Trehalose-6-phosphatase from Burkholderia pseudomallei==
-		+	<StructureSection load='6qj6' size='340' side='right'caption='[[6qj6]], [[Resolution\|resolution]] 1.74Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6qj6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QJ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QJ6 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">otsB, BPSL2411 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272560 BURPS])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trehalose-phosphatase Trehalose-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.12 3.1.3.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qj6 OCA], [http://pdbe.org/6qj6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qj6 RCSB], [http://www.ebi.ac.uk/pdbsum/6qj6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qj6 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q63SB3_BURPS Q63SB3_BURPS]] Removes the phosphate from trehalose 6-phosphate to produce free trehalose.[RuleBase:RU361117]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Burps]]
		+	[[Category: Large Structures]]
		+	[[Category: Trehalose-phosphatase]]
		+	[[Category: Gourlay, L J]]
		+	[[Category: Hydrolase]]
		+	[[Category: Trehalose-6-phosphatase]]

OCA at 06:22, 4 December 2019

OCA — Wed, 04 Dec 2019 06:22:13 GMT

←Older revision		Revision as of 06:22, 4 December 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6qj6 is ON HOLD	+	The entry 6qj6 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6qj6" [edit=sysop:move=sysop]

OCA — Wed, 30 Jan 2019 07:02:23 GMT

Protected "6qj6" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:02, 30 January 2019

OCA: New page: '''Unreleased structure''' The entry 6qj6 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 30 Jan 2019 07:02:21 GMT

New page: '''Unreleased structure''' The entry 6qj6 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6qj6 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]